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| 1 | H pylori (CagA) and Epstein-Barr virus infection in gastric carcinomas:Correlation with p53 mutation and c-Myc,Bcl-2 and Bax expression显示文摘AIM: To investigate the interrelationship between H pylori and Epstein-Barr virus (EBV) infection in the gastric carcinogenesis having in focus the p53 mutation and the c-Myc, Bcl-2 and Bax expression. METHODS: seventy-one gastric carcinoma tissues were assessed by polymerase chain reaction (PCR) for H pylori and in situ hybridization for EBV. c-Myc, Bcl-2 and Bax expression were detected by immunohistochemistry and single-stranded conformational polymorphism (SSCP) for p53 mutation. RESULTS: The positivity rates for H pylori and EBV were 94.4% and 8.45%, respectively. The majority of the cases displayed only the H pylori presence. All EBV positive cases were also H pylori positive. None infectious agent was observed in 5.55% of the cases. The intestinal type tumor was more frequent in the co-infected and non-infected groups. The female predominated in the non-infected group showing statistical significance (70.4% vs 29.6%, P=0.039). The Bcl-2 was only detected in the group exclusively infected by H pylori. However, c-Myc and Bax were detected in the three groups but with a low frequency in the co-infected group. Mutation of p53 was present in all groups, with the highest frequencies in the H pylori positive groups. CONCLUSION: The frequency of H pylori infection in gastric carcinomas was high. The presented data indicated that gastric carcinogenesis has different pathways depending of the presence of the two investigated infectious agents, suggesting a possible involvement of H pylori with apoptotic process. The low expression of c-Myc and Bax in the EBV-positive groups suggests that EBV may inhibit the expression of these proteins. Nevertheless, p53 mutation shows to be a relevant alteration, independent of both infectious agents. | Valeska Portela Lima Marcos Antonio Pereira de Lima Angela Rosa André Márcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Sílvia Helena Barem Rabenhorst | 2008 | World Journal of Gastroenterology2008，14，6： | 17 |
| 2 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 3 | No evidence of HPV DNA in esophageal squamous cell carcinoma in a population of Southern Brazil显示文摘AIM:To investigate the association between human papillomavirus(HPV)and esophageal squamous cell carcinoma(ESCC)in southern Brazil.METHODS:We studied 189 esophageal samples from125 patients from three different groups:(1)102 biopsies from 51 patients with ESCC,with one sample from the tumor and another from normal esophageal mucosa distant from the tumor;(2)50 esophageal biopsies from 37 patients with a previous diagnosis of head and neck squamous cell carcinoma(HNSCC);and(3)37 biopsies from esophageal mucosa with normal appearance from 37 dyspeptic patients,not exposed to smoking or alcohol consumption.Nested-polymerase chain reaction(PCR)with the MY09/11 and GP5/6 L1primers was used to detect HPV L1 in samples fixed in formalin and stored in paraffin blocks.All PCR reactions were performed with a positive control(cervicovaginal samples),with a negative control(Human Genomic DNA)and with a blank reaction containing all reagents except DNA.We took extreme care to prevent DNA contamination in sample collection,processing,and testing.RESULTS:The histological biopsies confirmed the diagnosis of ESCC in 52 samples(51 from ESCC group and 1 from the HNSCC group)and classified as well differentiated(12/52,23.1%),moderately differentiated(27/52,51.9%)or poorly differentiated(7/52,13.5%).One hundred twenty-eight esophageal biopsies were considered normal(51 from the ESCC group,42 from the HNSCC group and 35 from dyspeptic patients).Nine had esophagitis(7 from the HNSCC and 2 from dyspeptic patients).Of a total of 189 samples,only 6 samples had insufficient material for PCR analysis:1 from mucosa distant from the tumor in a patient with ESCC,3from patients with HNSCC and 2 from patients without cancer.In 183 samples(96.8%)GAPDH,G3PDH and/orβ-globin were amplified,thus indicating the adequacy of the DNA in those samples.HPV DNA was negative in all the 183 samples tested:52 with ESCC,9 with esophagitis and 122 with normal esophageal mucosa.CONCLUSION:There was no evidence of HPV infection in different ESCC from southern Brazil. | Luís Carlos Moreira Antunes Joo Carlos Prolla Antonio de Barros Lopes Marta Pires da Rocha Renato Borges Fagundes | 2013 | World Journal of Gastroenterology2013，19，39： | 4 |
| 4 | Transformation of wheat with high molecular weight subunit genes results in improved functional properties显示文摘 | Barro F Rooke L Bekes F Gras P Tatham A S Fido R Lazzeri P A Shewry P R Barcelo P | 1997 | Nat Biotechnol1997，15，12： | 2 |
| 5 | Efficacy of CFRP- based techniques for the flexural and shear strengthening of concrete beams 显示文摘 | Barros J A O Dias S J E Lima J 1 T | 2007 | Cement and Concrete Composites2007，29，3： | 1 |
| 6 | Fertility Choice in a Model of Economic Growth 显示文摘 | Barro R J Becker G S | 1989 | Econometric1989，2，： | 1 |
| 7 | Photosynthetic performance of two coffee species under drought显示文摘 | Matta F M D Maestri M Barros R S | 1998 | Photosyuthetica1998，，2： | 1 |
| 8 | A Reformulation of the Economic Theory of Fertility显示文摘 | Becker G S Barro R J | 1988 | Quarterly Journal of Economics1988，103，： | 1 |
| 9 | Do organizational incentives spur innovation?显示文摘 | Barros H M and Lazzarini S G | 2012 | Brazilian Administration Review2012，9，3： | 1 |
| 10 | incidence of postopera- tive residual neuromuscular blockade in the postanaesthesia care unit an observational multicentre study in Portugal 显示文摘 | Esteves S Martins M Barros F | 2013 | Eur J Anaesthesiol2013，30，5： | 1 |
| 11 | Non-malignant central airway obstruction 显示文摘 | Barros Casas D FemOndez-Bussy S Folch E | 2014 | Arch Bronconeumol2014，50，8： | 1 |
| 12 | Naturalistic pharmacogenetic study of treatment resistance to typical neuroleptics in European–Brazilian schizophrenics显示文摘 | Fabiana B. Kohlrausch Clarissa S. Gama Maria Inês Lobato Paulo Belmonte-de-Abreu Sidia M. Callegari-Jacques Alejandro Gesteira Francisco Barros ángel Carracedo Mara H. Hutz | 2008 | Pharmacogenetics and Genomics2008，，7： | 1 |
| 13 | The main benefits associated with health and safety management systems certification in Portuguese small and medium enterprises post quality management system certification 显示文摘 | Gilberto Santos S i ria Barros Fatima Mendes Nuno Lopes | 2013 | Safety Science2013，，51： | 1 |
| 14 | Mood disorder as a manifestation of primary hy- poparathyroidism:A case report显示文摘 | Regis G Rosa Alcina J S Barros Antonio RB de Lima | 2014 | Journal of Medical Case Reports2014，8，4： | 1 |
| 15 | Phenolic profile of seventeen Portuguese wild mushrooms显示文摘 | Josiana A. Vaz Lillian Barros Anabela Martins Jorge Sá Morais M. Helena Vasconcelos Isabel C.F.R. Ferreira | 2010 | LWT - Food Science and Technology2010，，1： | 1 |
| 16 | A golobal index for level of service ecaluation at airport passenger terminals显示文摘 | Correia A R Wirazinghe S C de Barros A G | 2008 | Transportation Research Part E2008，44，4： | 1 |
| 17 | Cutaneous myiasis of the breast: mammographic and us features-report of five cases显示文摘 | de Barros N D'Avila MS de Pace Bauab S | 2001 | Radiology2001，218，2： | 1 |
| 18 | The technical efficiency of the Japanese banks: non-radial directional performance measure- ment with undesirable output显示文摘 | Barros C P Managi S Matousek R | 2012 | Omega2012，40，1： | 1 |
| 19 | Separation of biodiesel and glycerol using ceramic membranes显示文摘 | SERGI GOMES M C PEREIRA N C DAVANTEL DE BARROS S T | 2010 | JournalofMembraneScience2010，352，2： | 1 |
| 20 | Serological survey of Babesia bovis, Babesia bigemina, and Anaplazma marginale antibodies in cattle from the semi -arid region of the state of Bahia, Brazil, by enzyme -linked immunosorbent assays 显示文摘 | Barros S Madmga C R Araujo F R | 2005 | Rio de Janeiro2005，100，6： | 1 |
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