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| 1 | Effects of transmitters and interleukin-10 on rat hepatic fibrosis induced by CCl_4显示文摘AIM: To study the effects of transmitters ET, AgII, PGI2,CGRP and GG on experimental rat hepatic fibrosis and the antifibrogenic effects of IL-10.METHODS: One hundred SD rats were randomly divided into 3 groups: control group (N): intraperitoneal injection a week; IL-10 treated group (E): besides same dosage of the third week. In the fifth, the seventh and the ninth week,rats in three groups were selected randomly to collect plasma and liver tissues. The levels of ET, AgII, PGI2, CGRP and GG were assayed by radioimmunoassay (RIA). The liver fibrosis was observed with silver staining.RESULTS: The hepatic fibrosis was developed with the increase of the injection frequency of CCl4. The ET, AgII, PGI2, CGRP The histological examination showed that the degrees of the rats liver fibrosis in group E were lower than those in group C.CONCLUSION: The transmitters ET, AgII, PGI2, CGRP and GG play a significant role in the rat hepatic fibrosis induced by CCl4. IL-10 has the antagonistic action on these transmitters and can relieve the degree of the liver fibrosis. | Xiao-Zhong Wang Li-Juan Zhang Dan Li Yue-Hong Huang Zhi-Xin Chen BinLi Department of Gastroenterology, The Affiliated Union Hospital, Fujian Medical University, Fuzhou, 350001, Fujian Province, China | 2003 | World Journal of Gastroenterology2003，9，3： | 33 |
| 2 | Analysis on Frequency and Density of Microsatellites in Coding Seauences of Several Eukarvotic Genomes显示文摘Microsatellites or simple sequence repeats (SSRs) have been found in most organisms during the last decade. Since large-scale sequences are being generated, especially those that can be used to search for microsatellites, the development of these markers is getting more convenient. Keeping SSRs in viewing the importance of the application, available CDS (coding sequences) or ESTs (expressed sequence tags) of some eukaryotic species were used to study the frequency and density of various types of microsatellites. On the basis of surveying CDS or EST sequences amounting to 66.6 Mb in silkworm, 37.2 Mb in fly, 20.8 Mb in mosquito, 60.0 Mb in mouse, 34.9 Mb in zebrafish and 33.5 Mb in Caenorhabditis elegans, the frequency of SSRs was 1/1.00 Kb in silkworm, 1/0.77 Kb in fly, 1/1.03 Kb in mosquito, 1/1.21 Kb in mousey 1/1.25 Kb in zebrafish and 1/1.38 Kb in C. Elegans. The overall average SSR frequency of these species is 1/1.07 Kb. Hexanucleotide repeats (64.5%-76.6%) are the most abundant class of SSR in the investigated species, followed by trimeric, dimeric, tetrameric, monomeric and pentameric repeats. Furthermore, the A-rich repeats are predominant in each type of SSRs, whereas G-rich repeats are rare in the coding regions. | BinLi QingyouXia ChengLu ZeyangZhou ZhonghuaiXiang | 2004 | Genomics, Proteomics & Bioinformatics2004，2，1： | 14 |
| 3 | Preparation and identification of anti-transforming growth factor β1 U1 small nuclear RNA chimeric ribozyme in vitro显示文摘AIM: To study the preparation and cleavage activity of antitransforming growth factor (TGF)β1 U1 small nuclear (sn)RNA chimeric hammerhead ribozymesin vitro.METHODS: TGFβ1 partial gene fragment was cloned into T-vector at the downstream of T7 promoter. 32p-labeled TGFβ1 partial transcripts as target RNA were transcribed in vitro and purified by denaturing polyacrylamide gel electrophoresis (PAGE). Anti-TGFβ1 ribozymes were designed by computer, then synthetic ribozyme fragments were cloned into the U1 ribozyme vector pZeoU1EcoSpe containing U1 snRNA promoter/enhancer and terminator.32p-labeled U1 snRNA chimeric ribozyme transcripts were gel-purified, incubated with target-RNAs at different conditions and autoradiographed after running denaturing PAGE.RESULTS: Active UlsnRNA chimeric ribozyme (U1Rz803)had the best cleavage activity at 50 °C; at 37 °C, it was active, Km=34.48 nmol/L, Kcat=0.14 min-1; while the point mutant ribozyme U1Rz803m had no cleavage activity, so these indicated the design of U1Rz803 was correct.CONCLUSION: U1Rz803 prepared in this study possessed the perfect specific catalytic cleavage activity. These results indicate U1 snRNA chimeric ribozyme U1Rz803 may suppress the expression of TGFβ1in vivo, therefore it may provide a new avenue for the treatment of liver fibrosis in the future. | Ju-ShengLin Yu-HuSong Xin-JuanKong BinLi Nan-ZhiLiu Xiao-LiWu You-XinJin | 2003 | World Journal of Gastroenterology2003，9，3： | 11 |
| 4 | Maxizyme-mediated specific inhibition on mutant-type p53 in vitro显示文摘AIM: To evaluate the specific inhibition of maxizyme directing against mutant-type p53 gene (mtp53) at codon 249 in exon 7 (AGG→AGT)in vitro.METHODS: Two different monomers of anti-mtp53maxizyme (maxizyme right MzR, maxizyme left MzL) and control mutant maxizyme (G5→A5) were designed by computer and cloned into vector pBSKU6 (pBSKU6MzR,pBSKU6MzL). After being sequenced, the restrictive endonuclease site in pBSKU6MzR was changed by PCR and then U6MzR was inserted into pBSKU6MzL, the recombinant vector was named pU6Mz and pU6asMz (mutant maxizyme).Mtp53 and wild-type p53 (wtp53) gene fragments were cloned into pGEN-T vector under the T7 promoter control.The 32p-labeled mtp53 transcript was the target mRNA. Cold maxizyme transcripts were incubated with 32p-labeled target RNA in vitro and radioautographed after denaturing polyacrylamide gel electrophoresis.RESULTS: In cell-free systems, pU6Mz showed a specific cleavage activity against target mRNA at 37 ℃ and 25 mM MgCL2. The cleavage efficiency of pU6Mz was 42 %, while pU6asMz had no inhibitory effect. Wtp53 was not cleaved by pU6Mz either.CONCLUSION: pU6Mz had a specific catalytic activity against mtp53 in cell-free system. These lay a good fundation for studying the effects of anti-mtp53 maxizyme in HCC cell lines. The results suggest that maxizyme may be a promising alternative approach for treating hepatocellular carcinoma containing mtp53. | Xin-JuanKong Yu-HuSong Ju-ShengLin Huan-JunHuang Nan-XiaWang Nan-ZhiLiu BinLi You-XinJin | 2003 | World Journal of Gastroenterology2003，9，7： | 6 |
| 5 | A NEW METHOD FOR DEOXIDIZATION AND CHLORINATION OF REFINED ILMENITE WITH HIGH MAGNESIA AND CALCIA CONTENTS显示文摘A new process is proposed to utilize refined ilmenite with high magnesia and calcia contents, which is the main secondary resource from vanadium-bearing titaniferous magnetite in Panzhihua, covering about 40% of the worlds titanium. The refined ilmenite was first deoxidized to separate the iron in the ore in order to enrich the titanium. The mechanism of chlorination of the titanium-rich slag was analyzed using the HSC chemistry software. Following deoxida-tion, a tandem fluidized bed was applied to chlorinating the titanium-rich slag. Modeling of the tandem fluidized bed ex-plains how this serial operation can effectively improve the convention ratio of TiO2 to TiCl4. | BinLi ZhangfuYuan WenbingLi CongXu | 2004 | China Particuology2004，2，2： | 4 |
| 6 | Failure Analysis of Power Electronic Devices and Their Applications under Extreme Conditions显示文摘Power electronic devices are the core components of modern power converters,not only for normal applications,but also for extreme conditions.Current design of power electronic devices require large redundancies for reliability.This results in huge volume and weight for a large-capacity power converter,especially for some extreme applications.Therefore,to optimize the power density,the reliability of power devices needs to be investigated first in order to obtain the accurate operational margin of a power device.Although much research on device failure analysis has been reported,there still lacks efficient failure evaluation methods.This paper first summarizes the current failure research.Then,a three-step failure analysis method of power electronic devices is proposed as:failure information collection,failure identification and mechanism,and failure evaluation.The physics-based modeling method is emphasized since it has a strong relationship with the device fundamentals.After that,power electronic device applications under extreme conditions are introduced and a design method of device under extreme conditions is proposed based on the thermal equilibrium idea.Finally,the challenges and prospects to improve the power device reliability under extreme conditions are concluded. | Yifei Luo Fei Xiao Bo Wang Binli Liu | 2016 | Chinese Journal of Electrical Engineering2016，2，1： | 2 |
| 7 | Fast protein tertiary structure retrieval based on global surface shape similarity显示文摘 | LeeSael BinLi DavidLa YiFang KarthikRamani RaifRustamov DaisukeKihara | 2008 | Proteins2008，，4： | 1 |
| 8 | Identifcation of up-regulated genes in human uterine leiomyoma by sup-pression subtractive hybridization显示文摘In searching for differentially expressed genes in human uterine leiomyomas (ULs), suppression sub-tractive hybridization was used to construct an UL up-regulated library, which turned out to represent 88genes. After two rounds of screening by reverse Northern analysis, twenty genes were proved to be up-regulated, including seventeen known genes and three genes with unknown function. All these genes werefirstly associated with UL. Three genes with notable difference were selected for Northern confirmationOur results proved the authenticity of the twenty genes. One gene named Phospholipase A2 (PLA2) showedup-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obviousexpression in prostate, testis, liver, heart and skeletal muscle. | BINLI YONGLIANZHANG 等 | 2002 | Cell Research2002，12，3： | 1 |
| 9 | Characterization of SARS-CoV-2-specific humoral immunity and its potential applications and therapeutic prospects显示文摘Coronavirus disease 2019(COVID-19),caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is an ongoing pandemic that poses a great threat to human health worldwide.As the humoral immune response plays essential roles in disease occurrence and development,understanding the dynamics and characteristics of virus-specific humoral immunity in SARS-CoV-2-infected patients is of great importance for controlling this disease.In this review,we summarize the characteristics of the humoral immune response after SARS-CoV-2 infection and further emphasize the potential applications and therapeutic prospects of SARSCoV-2-specific humoral immunity and the critical role of this immunity in vaccine development.Notably,serological antibody testing based on the humoral immune response can guide public health measures and control strategies;however,it is not recommended for population surveys in areas with very low prevalence.Existing evidence suggests that asymptomatic individuals have a weaker immune response to SARS-CoV-2 infection,whereas SARS-CoV-2-infected children have a more effective humoral immune response than adults.The correlations between antibody(especially neutralizing antibody)titers and protection against SARS-CoV-2 reinfection should be further examined.In addition,the emergence of cross-reactions among different coronavirus antigens in the development of screening technology and the risk of antibody-dependent enhancement related to SARS-CoV-2 vaccination should be given further attention. | Jiaxin Zheng Yingying Deng Zhenyu Zhao Binli Mao Mengji Lu Yong Lin Ailong Huang | 2022 | Cellular & Molecular Immunology2022，19，2： | 1 |
| 10 | Purification and characterization of a cellulose-binding β-glucosidase from cellulosedegrading cultures of Phanerochaete chrysosporium显示文摘 | ELENA S LYMAR BINLI | 1995 | Applied and Environmental Microbiology1995，，8： | 1 |
| 11 | Textual Metaphor from the Perspective of Relator显示文摘 | Qingshun He Bingjun Yang Binli Wen | 2015 | Australian Journal of Linguistics2015，，： | 1 |
| 12 | Thermal degradation kinetics of rigid polyurethane foams blown with water显示文摘 | Xiao Binli Hong Bincao Zhang Yi | 2006 | Journal of Applied Polymer Science2006，102，5： | 1 |
| 13 | Gaps in the 2010 measles SIA coverage among migrant children in Beijing: evidenee from a parental survey 显示文摘 | Xiaojiang Hu Suowei Xiao Binli Chen | 2012 | Vaccine2012，30，39： | 1 |
| 14 | Macrophage migration inhibitory factor contributes angiogenesis by up‐regulating IL‐8 and correlates with poor prognosis of patients with primary nasopharyngeal carcinoma显示文摘 | BingLiao Bi‐lingZhong ZhiLi Xiao‐yingTian YangLi BinLi | 2010 | Oncol2010，，7： | 1 |
| 15 | Special regulatory T‐cell review: FOXP3 biochemistry in regulatory T cells – how diverse signals regulate suppression显示文摘 | BinLi Mark I.Greene | 2007 | Immunology2007，，1： | 1 |
| 16 | Joint neurodevelopmental and Behavioral effects of nolylphenol and estradiol on F1 male rats 显示文摘 | Yu J Fan QY Binli H | 2013 | Int J Environ Health Res2013，23，4： | 1 |
| 17 | Joint neurodevelopmental and behavioral effects of nonylphenol and estradiol on F (1) male rats显示文摘 | Yu J Fan Q Y Binli H | 2013 | Int J Environ Health Res2013，23，4： | 1 |
| 18 | Thermodynamic Evaluation of Cr_2O_3-Al_2O_3 Refractory Corrosion by Smelting Reduction Ironmaking Slag显示文摘The interfacial reactions of Cr2O3- Al2O3 refractory materials with smelting reduction ironmaking slag were investigated by thermodynamic modeling. The calculation results indicated that different spinel phases were produced after the interactions between the slag and three types of refractory materials( 10Cr2O3- 90Al2O3,50Cr2O3- 50Al2O3 and 90Cr2O3- 10Al2O3) at 1 500℃. Increased contents of Al2O3 and Cr2O3in the remained liquid slag after interaction implied the dissolution of refractory. Thermodynamic modeling predicted that the process of dissolution might lead to much deeper penetration into the refractory materials by the present compositional slag. Accordingly,corrosion testing was also conducted to validate the thermodynamic prediction. | ZHAO Shixian CAI Binli LI Pengtao SUN Honggang WANG Gang | 2015 | China's Refractories2015，24，2： | 1 |
| 19 | Joint neurodevelopmental and behavioral effects of nonylphenol and estradiol on F(1)male rats显示文摘 | Yu J Fan QY Binli H | 2013 | International Journal of Environmental Health Research2013，23，4： | 1 |
| 20 | Two new monoterpenes from Sibi- raea angustata显示文摘 | BinLi Jung-Bum Lee Kyoko Hayashi etc | 2010 | J Nat Med2010，64，： | 1 |
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