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| 1 | 百日咳毒素S1亚单位的分子修饰及在重组杆状病毒中的表达显示文摘目的:百日咳毒素既是百日咳杆菌的主要毒性因子,又是重要的保护性抗原,其S1亚单位具有ADP核糖转移酶活性和免疫保护性决定簇。为高效表达前构建的一个丧失酶活性但仍保持保护决定簇的S1变异子,开展了本研究。方法:通过添加人流感病毒血凝素基因信号序列或嵌膜序列的方法,对天然和变异S1亚单位基因片段作了遗传学修饰。然后使用重组杆状病毒技术使这些S1亚单位在昆虫细胞和昆虫幼虫中实现了高水平表达。结果:这些重组S1的表达水平介于每万个昆虫卵细胞018~193μg或每只昆虫幼虫046~498mg之间。天然信号肽和HA信号肽均可完整表达,但后者的切割效率(61%~81%)比前者(16%~19%)更高。所有带信号肽的S1亚单位均呈异质性表达(双带)。结论:重组S1亚单位的表达是高效和正确的,表达的异质性是由于信号肽切割不完全的缘故。 | 于康震 Burnette WN Kaslow HR Mar VL Ahmad S Yilma TD | 1999 | 中国免疫学杂志1999，15，2： | 4 |
| 2 | The potential role of resistin in atherogenesis 显示文摘 | BURNETT M S LEE C W KINNAIRD T D | 2005 | Atherosclerosis2005，182，： | 1 |
| 3 | The Mechanical Properties of Wear-resistant Coatings:I Modelling of Hardness Havior显示文摘 | Rickerby D S | 1987 | Thin Solid Film s1987，148，： | 1 |
| 4 | Adjusting for temporal variation in analysis of parallel time series of health and environmental variables 显示文摘 | CAKMAK S BURNETT R KREWSKI D | 1998 | J Expos Anal Environ Epidemiol1998，8，2： | 1 |
| 5 | Pexophagy: the selective degradation of peroxisomes 显示文摘 | Till A Lakhani R Burnett S F | 2012 | International Journal of Cell Biology2012，2012，51： | 1 |
| 6 | The relationship between hardness and scratch adhesion显示文摘 | Burnett P J Rickerby D S | 1987 | Thin Solid Films1987，154，： | 1 |
| 7 | The IgA heavy-chain gene family in rabbit:cloning and sequence analysis of 13 C alpha genes显示文摘 | Burnett R C Hanly W C Zhai S K | 1989 | EMBO1989，8，13： | 1 |
| 8 | Indications for patellar resurfacing intotal knee ailhroplasty 显示文摘 | Burnett R S Bourne R B | 2004 | Instr Course Led2004，53，： | 1 |
| 9 | Indications for patellar resurfacing in total knee arthroplasty显示文摘 | Burnett R S Bourne R B | 2003 | The Journal of Bone and Joint Surgery2003，85，4： | 1 |
| 10 | A three-dimensional acoustic infinite element based on a prolate spheroidal multiple expansion 显示文摘 | Burnett D S | 1994 | Journal of the Acoustical Society of America1994，96，5： | 1 |
| 11 | Conceptualizing guilt in the consumer decision-making process 显示文摘 | Burnett M S Lundford D A | 1994 | Consum Mark1994，11，3： | 1 |
| 12 | Diagnosis and management of acute myeloid leukemia in adults:recommendations from an international expert panel,on behalf of the European LeukemiaNet显示文摘 | Dhner H Estey EH Amadori S Appelbaum FR Büchner T Burnett AK Dombret H Fenaux P Grimwade D Larson RA Lo-Coco F Naoe T Niederwieser D Ossenkoppele GJ Sanz MA Sierra J Tallman MS Lwenberg B Bloomfield CD | 2010 | {H}Blood2010，，03： | 1 |
| 13 | Cross-sectional associations of resistin, coro nary heart disease, and insulin resistance显示文摘 | BURNETT M S DEVANEY J M ADENIKA R J | 2006 | J Clin Endocrinol Metab2006，91，： | 1 |
| 14 | Posterior-stabilized versus cruciate-retaining total knee arthroplasty : balancing the gap 显示文摘 | Tanzer M Smith K Burnett S | 2002 | J Arthroplasty2002，7，： | 1 |
| 15 | Extremely high multiplicities in high-energy nucleus-nucleus collisions显示文摘 | Burnett T H Dake S Fuki M | 1983 | Phys Rev Lett1983，50，26： | 1 |
| 16 | The relationship between hardness and scratch adhession显示文摘 | BURNETT P J RICKERB D S | 1987 | Thin Solid Films1987，154，1： | 1 |
| 17 | Polychlorinatedbiphenyls (PCBs)in the British environment:Sinks,sources and temporal trends显示文摘 | Harrad S J Sewart A P Alcock R Boumphrey R Burnett V Davidson R D Halsall C Sanders G Waterhouse K | 1994 | Environmental Pollution1994，85，： | 1 |
| 18 | Regulation of cterminaland intact FGF-23 by dietary phosphatein men and women显示文摘 | Burnett S A M Gunawardene S C Bringhurst F R | 2006 | Journal of Bone and mineral Research2006，21，8： | 1 |
| 19 | Training effects in mice after long-term voluntary exercise显示文摘 | DAVIDSON S R BURNETT M LAURIE H G | 2006 | Med Sci Sports Exe2006，38，2： | 1 |
| 20 | Fundulus as the premier teleost model in environmental biology: Opportunities for new insights using genomics显示文摘 | BURNETT K Q BAIN L J BALDWIN W S | 2007 | Comparative Biochemistry and Physiology Part D: Genomics Proteomics2007，2，4： | 1 |
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