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| 1 | Alleviated mucosal and neuronal damage in a rat model of Crohn's disease显示文摘AIM:To establish a rat model suitable to investigate the repetitive relapsing inflammations(RRI)characteristic to Crohn’s disease.METHODS:Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid(TNBS).RRI were mimicked by repeating administrations of TNBS.Tissue samples were taken from control,once,twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation.The means of the ulcerated area were measured to evaluate the macroscopic mu-cosal damage.The density of myenteric neurons was determined on whole mounts by Hu C/Hu D immunohistochemistry.Heme oxygenase-1(HO-1)expression was evaluated by molecular biological techniques.RESULTS:TNBS-treated rats displayed severe colitis,but the mortality was negligible,and an increase of body weight was characteristic throughout the experimental period.The widespread loss of myenteric neurons,and marked but transient HO-1 up-regulation were demonstrated after the first TNBS administration.After repeated doses the length of the recovery time and extent of the ulcerous colonic segments were markedly decreased,and the neuronal loss was on a smaller scale and was limited to the inflamed area.HO-1 m RNA level was notably greater than after a single dose and overexpression was sustained throughout the timepoints examined.Nevertheless,the HO-1protein up-regulation after the second TNBS treatment proved to be transient.Following the third treatment HO-1 protein expression could not be detected.CONCLUSION:Experimentally provoked RRI may exert a protective preconditioning effect against the mucosal and neuronal damage.The persistent up-regulation of HO-1 m RNA expression may correlate with this. | Petra Talapka Lajos István Nagy Alexandra Pál Marietta Zita Poles Anikó Berkó Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2014 | World Journal of Gastroenterology2014，20，44： | 3 |
| 2 | Non-operativemanagementoftheuhrasoni-callyevaluated appendicela mass显示文摘 | Bagi P Oueholm S | 2008 | Surgeyr2008，101，： | 1 |
| 3 | Sunitinib and PF-562,271 (FAKlPyk2 inhibitor) effectively block growth and recovery of human hepatocellular carcinoma in a rat xenograft model显示文摘 | Bagi C M Christensen J Cohen 0 P | 2009 | Cancer Bioi Ther2009，8，9： | 1 |
| 4 | Myocardial edema as detected by pre-contrast T1 and T2 CMR delineates area at risk associated with acute myocardial infarction显示文摘 | Ugander M Bagi P S Oki A J | 2012 | JACC: Cardiovascular Imaging2012，5，6： | 1 |
| 5 | Renal inflammatory myofibroblastic tumor: a rare tumor indistinguishable from renal cell carcinoma-report of two cases 显示文摘 | Heerwagen ST Jensen C Bagi P | 2007 | Acta Radiol2007，48，10： | 1 |
| 6 | Effect of estrogen deficiency on cancellous and cortical bone structure and strength of the femoral neck in rats显示文摘 | Bagi CM Ammann P Rizzoli R | 1997 | CalcifTissue Int1997，61，4： | 1 |
| 7 | Home uroflowmetry by means of the Da Capo home uroflowmeter 显示文摘 | Jorgensen JB Jacobsen HL Bagi P | 1998 | Eur Urol1998，33，1： | 1 |
| 8 | Non - operative management of the ultrasoni- cally evaluated appendiceal mass 显示文摘 | Bagi P Oueholm S | 1987 | Surgery1987，101，： | 1 |
| 9 | Nonoperative management of the ultrasonically eval- uated appendiceal mass显示文摘 | Bagi P Dueholm S | 1987 | Surgery1987，101，5： | 1 |
| 10 | An Immunohistochemical evaluation of cell adhesion molecules in human dental pulp after tooth preparary luting cements显示文摘 | BAGIS B ATILLA P CAKAR N | 2009 | Oral Surg Oral Med Oral Pathol Oral Radiol Endod2009，107，1： | 1 |
| 11 | Effedcts of ejaculation by penile vibratory stimulation on bladder capacity in men with spinal cord lesions显示文摘 | Laessoe L Sonksen J Bagi P | 2003 | J Urol2003，169，6： | 1 |
| 12 | Effects of ejaculation by penile vibratory stimulation on bladder reflex activity in a spinal cord injured man 显示文摘 | Laessoe L Sonksen J Bagi P | 2001 | J Urol2001，166，2： | 1 |
| 13 | An immunohistochemical e- valuation of cell adhesion molecules in human dental pulp after tooth preparation and application of temporary luting cements 显示文摘 | Bagis B Atilla P Carkar N | 2009 | Oral Surg Oral Med Oral Pathol Oral Radiol Endod2009，107，1： | 1 |
| 14 | Myocardial edema as detected by pre-contrast T1 and I2 CMR delineates area at risk associated with acute myocardial infarction显示文摘 | Ugander M Bagi P S Oki A J | 2012 | JACC : Cardiovascular Imaging2012，5，6： | 1 |
| 15 | Onabotuli- num toxin A (botox) in the treatment of neurogenic bladder overaetivity显示文摘 | ROHRSTED M NORDSTEN CB BAGI P | 2012 | Nephrourol Mon2012，4，2： | 1 |
| 16 | Botulinum toxin A for treatment of neurogenic detrusor overactivity and incontinence in patients with spinal cord lesions 显示文摘 | Bagi P Biering-Sorensen F | 2004 | Scand J Urol Nephrol2004，38，6： | 1 |
| 17 | Prevalence and characterization of shiga toxin-producing Escherichia coil in swine feces recovered in the National Animal Health Monitoring System's Swine 2000 study 显示文摘 | Fratamico P M Bagi L K Bush E J | 2004 | Appl Environ Microbiol2004，70，12： | 1 |
| 18 | Effect of estrogen deficiency on cancellous and cortical bone structure and strength of the femoral neck in rats显示文摘 | Bagi CM Ammann P Rizzoli R | 1997 | Calcif Tissue Int1997，61，4： | 1 |
| 19 | Gut region-specific TNFR expression:TNFR2 is more affected than TNFR1 in duodenal myenteric ganglia of diabetic rats显示文摘BACKGROUND Cytokines are essential in autoimmune inflammatory processes that accompany type 1 diabetes.Tumor necrosis factor alpha plays a key role among others in modulating enteric neuroinflammation,however,it has a dual role in cell degeneration or survival depending on different TNFRs.In general,TNFR1 is believed to trigger apoptosis,while TNFR2 promotes cell regeneration.The importance of the neuronal microenvironment has been recently highlighted in gut region-specific diabetic enteric neuropathy,however,the expression and alterations of different TNFRs in the gastrointestinal tract has not been reported.AIM To investigate the TNFR1 and TNFR2 expression in myenteric ganglia and their environment in different intestinal segments of diabetic rats.METHODS Ten weeks after the onset of hyperglycemia,gut segments were taken from the duodenum,ileum and colon of streptozotocin-induced(60 mg/body weight kg i.p.)diabetic(n=17),insulin-treated diabetic(n=15)and sex-and age-matched control(n=15)rats.Myenteric plexus whole-mount preparations were prepared from different gut regions for TNFR1/HuCD or TNFR2/HuCD double-labeling fluorescent immunohistochemistry.TNFR1 and TNFR2 expression was evaluated by post-embedding immunogold electron microscopy on ultrathin sections of myenteric ganglia.TNFRs levels were measured by enzyme-linked immunosorbent assay in muscle/myenteric plexus-containing(MUSCLE-MP)tissue homogenates from different gut segments and experimental conditions.RESULTS A distinct region-dependent TNFRs expression was detected in controls.The density of TNFR1-labeling gold particles was lowest,while TNFR2 density was highest in duodenal ganglia and a decreased TNFRs expression from proximal to distal segments was observed in MUSCLE-MP homogenates.In diabetics,the TNFR2 density was only significantly altered in the duodenum with decrease in the ganglia(0.32±0.02 vs 0.45±0.04,P<0.05),while no significant changes in TNFR1 density was observed.In diabetic MUSCLE-MP homogenates,both TNFRs levels significantly decreased in the duodenum(TNFR1:4.06±0.65 vs 20.32±3.1,P<0.001;TNFR2:11.72±0.39 vs 15.91±1.04,P<0.01),which markedly influenced the TNFR2/TNFR1 proportion in both the ganglia and their muscular environment.Insulin treatment had controversial effects on TNFR expression.CONCLUSION Our findings show diabetes-related region-dependent changes in TNFR expression and suggest that TNFR2 is more affected than TNFR1 in myenteric ganglia in the duodenum of type 1 diabetic rats. | Bence Pál Barta Benita Onhausz Afnan AL Doghmi Zita Szalai János Balázs Mária Bagyánszki Nikolett Bódi | 2023 | World Journal of Diabetes2023，14，1： | 0 |
| 20 | Diabetes-related intestinal region-specific thickening of ganglionic basement membrane and regionally decreased matrix metalloproteinase 9 expression in myenteric ganglia显示文摘BACKGROUND The importance of the neuronal microenvironment has been recently highlighted in gut region-specific diabetic enteric neuropathy. Regionally distinct thickening of endothelial basement membrane(BM) of intestinal capillaries supplying the myenteric ganglia coincide with neuronal damage in different intestinal segments. Accelerated synthesis of matrix molecules and reduced degradation of matrix components may also contribute to the imbalance of extracellular matrix dynamics resulting in BM thickening. Among the matrix degrading proteinases, matrix metalloproteinase 9(MMP9) and its tissue inhibitor(TIMP1) are essential in regulating extracellular matrix remodelling.AIM To evaluate the intestinal segment-specific effects of diabetes and insulin replacement on ganglionic BM thickness, MMP9 and TIMP1 expression.METHODS Ten weeks after the onset of hyperglycaemia gut segments were taken from the duodenum and ileum of streptozotocin-induced diabetic, insulin-treated diabetic and sex-and age-matched control rats. The thickness of BM surrounding myenteric ganglia was measured by electron microscopic morphometry. Wholemount preparations of myenteric plexus were prepared from the different gut regions for MMP9/TIMP1 double-labelling fluorescent immunohistochemistry. Post-embedding immunogold electron microscopy was applied on ultrathin sections to evaluate the MMP9 and TIMP1 expression in myenteric ganglia and their microenvironment from different gut segments and conditions. The MMP9 and TIMP1 messenger ribonucleic acid(m RNA) level was measured by quantitative polymerase chain reaction.RESULTS Ten weeks after the onset of hyperglycaemia, the ganglionic BM was significantly thickened in the diabetic ileum, while it remained intact in the duodenum. The immediate insulin treatment prevented the diabetes-related thickening of the BM surrounding the ileal myenteric ganglia. Quantification of particle density showed an increasing tendency for MMP9 and a decreasing tendency for TIMP1 from the proximal to the distal small intestine under control conditions. In the diabetic ileum, the number of MMP9-indicating gold particles decreased in myenteric ganglia, endothelial cells of capillaries and intestinal smooth muscle cells, however, it remained unchanged in all duodenal compartments. The MMP9/TIMP1 ratio was also decreased in ileal ganglia only. However, a marked segment-specific induction was revealed in MMP9 and TIMP1 at the m RNA levels.CONCLUSION These findings support that the regional decrease in MMP9 expression in myenteric ganglia and their microenvironment may contribute to extracellular matrix accumulation, resulting in a region-specific thickening of ganglionic BM. | Nikolett Bódi Diána Mezei Payal Chakraborty Zita Szalai Bence Pál Barta János Balázs Zsolt Rázga Edit Hermesz Mária Bagyánszki | 2021 | World Journal of Diabetes2021，12，5： | 0 |
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