|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Histone acetyltransferase p300 regulates the expression of human pituitary tumor transforming gene (hPTTG)显示文摘The human pituitary tumor transforming gene (hPTTG) serves as a marker for malignancy grading in several cancers. hPTTG is involved in multiple cellular pathways including cell transformation, apoptosis, DNA repair, genomic instability, mitotic control and angiogenesis induction. However, the molecular mechanisms underlying hPTTG regulation have not been fully explored. In this study, we found that overexpression of histone acetyltransferase (HAT) p300 upregulated hPTTG at the levels of promoter activity, mRNA and pro- tein expression. Moreover, the HAT activity of p300 was critical for its regulatory function. Chromatin immunoprecipitation (ChIP) analysis revealed that overexpression of p300 elevated the level of histone H3 acetylation on the hPTTG promoter. Additionally, the NF-Y sites at the hPTTG promoter exhibited a synergistic effect on upregulation of hPTTG through interacting with p300. We also found that treatment of 293T cells with the histone deacetylase (HDAC) inhibitor Trichostatin A (TSA) increased hPTTG promoter activity. Meanwhile, we provided evidence that HDAC3 decreased hPTTG promoter activity. These data implicate an important role of the histone acetylation modification in the regulation of hPTTG. | Tian Li Hui Huang Binlu Huang Baiqu Huang Jun Lu | 2009 | Journal of Genetics and Genomics2009，36，6： | 9 |
| 2 | The genomic landscapes of histone H3-Lys9 modifications of gene promoter regions and expression profiles in human bone marrow mesenchymal stem cells显示文摘Mesenchymal stem cells (MSCs) of nonembryonic origins possess the proliferation and multilineage differentiation potentials. It has been established that epigenetic mechanisms could be critical for determining the fate of stem cells, and MSCs derived from different origins exhibited different expression profiles individually to a certain extent. In this study, ChIP-on-chip was used to generate ge-nome-wide histone H3-Lys9 acetylation and dimethylation profiles at gene promoters in human bone marrow MSCs. We showed that modifications of histone H3-Lys9 at gene promoters correlated well with mRNA expression in human bone marrow MSCs. Functional analysis revealed that many key cellular pathways in human bone marrow MSC self-renewal, such as the canonical signaling pathways, cell cycle pathways and cytokine related pathways may be regulated by H3-Lys9 modifications. These data suggest that gene activation and silencing affected by H3-Lys9 acetylation and dimethylation, respectively, may be essential to the maintenance of human bone mar-row MSC self-renewal and multi-potency. | Jiang Tan Hui Huang Wei Huang Lin Li Jianhua Guo Baiqu Huang Jun Lu | 2008 | Journal of Genetics and Genomics2008，35，10： | 6 |
| 3 | HDAC Inhibitors:A Potential New Category of Anti-Tumor Agents显示文摘Over the past years,it has been found that the epigenetic silence of tumor suppressor genes induced by overexpression of histone deacetylases (HDACs) plays an important role in carcinogenesis. Thus,HDAC inhibitors have emerged as the accessory therapeutic agents for multiple human cancers,since they can block the activity of specific HDACs,restore the expression of some tumor suppressor genes and induce cell differentiation,growth arrest and apoptosis. To date,the precise mechanisms by which HDAC inhibitors induce cell death have not yet been fully elucidated and the roles of individual HDAC inhibitors have not been identified. Moreover,the practical uses of HDAC inhibitors in cancer therapy,as well as their synergistic effects with other therapeutic strategies are yet to be evaluated. In this review article,we discuss briefly the recent advances in studies of the developments of anti-cancer HDAC inhibitors and their potential clinical value. | Lina Pan Jun Lu Baiqu Huang | 2007 | Cellular & Molecular Immunology2007，4，5： | 4 |
| 4 | Rb Protein is Essential to the Senescence-Associated Heterochromatic Foci Formation Induced by HMGA2 in Primary WI38 Cells显示文摘细胞的老朽是提供一个障碍给肿瘤的转变的房间周期拘捕的一张不可逆的表格。Rb (Retinoblastoma ) 小径的正直为为衰老的状态的稳定性提供一个分子的基础的联系老朽的 heterochromatin foci (SAHF ) 的形成是必要的。令人惊讶地,尽管高活动性组 A2 蛋白质(HMGA2 ) 能支持 tumorigenesis 并且在肿瘤房间禁止 Rb 功能, HMGA2 的高级表示是足够的在主要房间导致 SAHF 形成。决定 Rb 蛋白质是否在导致 HMGA2 的 SAHF 形成是必要的因此变得重要。在这研究,我们由 HMGA2,典型衰老的标记在被看见的宫外的表示建立了细胞的老朽和 SAHF 汇编 WI38 房间模型,包括 p53, p21 和 p16 的著名 upregulation,并且提高了 SA -- 和 E2F 目标基因的 downregulation 染色的牛乳糖。我们然后证明 Rb 小径禁止者 E7 蛋白质能部分在 WI38 房间在 HMGA2 表示以后废除 SAHF 形成的能力,显示 Rb 是为导致 HMGA2 的 SAHF 形成的一个关键因素。然而, Rb 弄空完全没救 HMGA2 导致的房间生长拘捕,建议 Rb 不是为在 WI38 房间的导致 HMGA2 的老朽的一条独占的小径。 | Xi Shi Baoqing Tian Lingxia Liu Yanyan Gao Chi Ma Namusamba Mwichie Wenlong Ma Liping Han Baiqu Huang Jun Lu Yu Zhang | 2013 | Journal of Genetics and Genomics2013，40，8： | 4 |
| 5 | Reversible Histone Acetylation Involved in Transcriptional Regulation of WT1 Gene显示文摘验证参与可逆嘘在人的 Wilm 的肿瘤的 transcriptional 规定的一 acetylation 1 基因(WT1 ) ,我们分析了角色嘘一 deacetylases (HDAC ) 并且嘘在这个 epigenetic 过程的一 acetyltransferase。检验的六 HDAC (HDAC1-6 ) , HDAC4 和 HDAC5 被发现有重要镇压在 WT1 记者基因的活动完成,由酶记者试金和量的即时反向的抄写聚合酶链反应揭示了试金。酶记者试金证明一 acetyltransferase p300 能在 WT1 记者的激活与转录因子 Sp1, c-Myb,和 Ets-1 抵抗 HDAC4/HDAC5-mediated 压抑和那 p300/CBP synergized 的 hist。染色质免疫降水实验证明 p300 支持 acetylation 水平嘘在 WT1 intronic 增强子的一 H3。把数据基于这些,我们为参与建议了一个假想模型可逆嘘在 WT1 基因的 transcriptional 规定的一 acetylation。这研究提供进一步的卓见进联系的 WT1 基因和 WT1 的 transcriptional 规定的机制疾病处理。 | Yangguang SHAO Jun LU Cao CHENG Liguo CUI Guoping ZHANG Baiqu HUANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，12： | 4 |
| 6 | hELP3 Subunit of the Elongator Complex Regulates the Transcription of HSP70 Gene in Human Cells显示文摘人的 Elongator 建筑群显著地类似于它在几个方面的酵母对应物。在以前的研究,我们分析了人的延伸蛋白质的功能 3 (hELP3 ) 由使用的人的 Elongator 的子单元一在里面 vivo 酵母 complementationsystem.However,为在在人的房间调整基因表达的 hELP3 功能的直接证据没被获得。在这研究,我们使用了 hELP3 反感觉寡核苷酸禁止者将 hELP3gene 表示击倒在人的 293T 房间调查它的函数。结果证明 hELP3 mRNA 和蛋白质的那特定的减小引起了 HSP70-2 基因表达的重要抑制,并且这被伴随由嘘一 H3 hypoacetylation 和减少的 RNA 聚合酶 II 在 theHSP70-2 的密度基因。而且,数据也证明 hELP3 施加了 transcriptional 规章在 HSP70-2 基因上通过它的存在直接工作。在这份报告介绍的数据在人的房间在 HSP70-2 基因 transcriptionalelongation 提供进一步的卓见和 hELP3 的函数的直接证据。 | Qiuju HAN Xiaozhe HOU Dongmei SU Lina PAN Jizhou DUAN Liguo CUI Baiqu HUANG Jun LU | 2007 | Acta Biochimica et Biophysica Sinica2007，39，6： | 3 |
| 7 | HDAC4 inhibits the transcriptional activation of mda-7/IL-24 induced by Sp1显示文摘Melanoma differentiation-associated gene/interleukin-24(mda-7/IL-24)is a cytokine that can activate monocytes and T helper 2 cells.The expression of mda-7/IL-24 gradually fades with the progression of melanoma,and it is undetectable at the metastatic stage.Ectopic expression of mda-7/IL-24 selectively suppresses growth and induces apoptosis in cancer cells with little harm to normal cells.However,the transcriptional regulation of the mda-7/IL-24 gene has not been extensively studied.In this study,we show that the expression of mda-7/IL-24 was upregulated by the histone deacetylase(HDAC)inhibitors trichostatin A(TSA)and sodium butyrate(NaBu),whereas it was downregulated by HDAC4.We also found that the histone acetylation level and the binding of the transcriptional factor Sp1 to the mad-7 promoter were reduced upon HDAC4 treatment.Moreover,the HDAC inhibitor TSA induced histone hyperacetylation and stimulated Sp1 binding to the mda-7/IL-24 promoter,which in turn enhanced the expression of mda-7/IL-24.Therefore,we conclude that histone acetylation modification plays an important role in the regulation of mda-7/IL-24 and that the transcription factor Sp1 participates in this process. | Lina Pan Hong Pan Hao Jiang Juan Du Xiuli Wang Baiqu Huang Jun Lu | 2010 | Cellular & Molecular Immunology2010，7，3： | 1 |
| 8 | Ras protein participated in histone acetylation-mediated cell cycle control in Physarum polycephalum显示文摘In this paper, we demonstrate that in Physarum polycephalum, a naturally synchronized slime mold, histone deacetylase (HDAC) inhibitor Trichostatin A (TSA), arrestes the cell cycle at the checkpoints of S/G2, G2/M and mitosis exit, and influences the transcription of two ras genes Ppras1 and Pprap1, as well as the Ras protein level. Antibody neu-tralization experiment using anti-Ras antibody treatment showed that Ras protein played an important role in cell cycle checkpoint control through regulation of the level of Cyclin B1, suggesting that Ras protein might be a key factor for histone acetylation-mediated cell cycle regulation in P. polycephalum. | LI Xiaoxue LU Jun ZHAO Yanmei WANG Xiuli HUANG Baiqu | 2005 | Chinese Science Bulletin2005，50，16： | 0 |
| 9 | Roles of histones and nucleosomes in gene transcription显示文摘This article reviews the latest research developments in the field of eukaryotic gene regulation by the structural alterations of chromatin and nucleosomes. The following issues are briefly addressed: (i) nucleosome and histone modifications by both the ATP-dependent remodeling complexes and the histone acetyltransferases and their roles in gene activation; (ii) competitive binding of histones and transcription factors on gene promoters, and transcription repression by nucleosomes; and (iii) influences of linker histone HI on gene regulation. Meanwhile, the significance and impact of these new research progresses, as well as issues worthwhile for further study are commented. | Baiqu Huang Qinhua Zeng Xiaohui Bi Yuhong Wang Yuxin Li | 2001 | Chinese Science Bulletin2001，46，3： | 0 |
| 10 | Actin and nuclear myosin I are associated with RNAP II and function in gene transcription显示文摘The presence of actin in the nucleus as well as its functions in various nuclear processes has been made clear in the past few years. Actin is known to be a part of chromatin-remodeling complexes BAF, which are required for maximal ATPase activity of the Brg1 component of the BAF complex. Moreover, the essential roles of actin in transcription mediated by RNA polymerases Ⅰ,Ⅱ and Ⅲ have been demonstrated recently. On the other hand, a myosin Ⅰ isoform, which contains a unique NH2-terminal extension for nucleus localization, has been specifically localized in nucleus. As is well known, myosin Ⅰ is an actin-binding protein and plays an important role in various cellular activities. Though actin and nuclear myosin Ⅰ (NM Ⅰ) have been implicated to play distinct roles in gene expression, there has been no evidence for the actin-myosin interaction that might be involved in gene transcription mediated by RNA polymerase Ⅱ (RNAP Ⅱ). Here we show evidence that both actin and NM Ⅰ are associated with RNAP Ⅱ in nucleus by using co-localization and co-IP assays, and they may act together on gene transcription. The antibodies against β-actin or NM Ⅰ can block RNA synthesis in a eukaryotic in vitro transcription system with template DNA comprising the promoter and the coding region of human autocrine motility factor receptor (hAMFR) gene; the antibodies pre-adsorbed with purified actin and NM Ⅰ have no effect in transcriptional inhibition, indicating that the inhibition of transcription by anti-actin and anti-NM I is specific. These results suggest a direct involvement of actin-myosin complexes in regulating transcription. It also implicates that actin and NM Ⅰ may co-exist in a same complex with RNAP Ⅱ and the interaction of RNAP Ⅱ with actin and NM Ⅰ functions in the RNAP Ⅱ-mediated transcription. | ZHU XiaoJuan HUANG BaiQu WANG XingZhi HAO Shui ZENG XianLu | 2007 | Chinese Science Bulletin2007，52，6： | 0 |
| 11 | Progress in studies of angio-statin and its anti-tumor effects显示文摘Angiostatin is a 38 ku circulating angiogenesis inhibitor purified from the serum and urine of mice bearing a murine Lewis lung carcinoma. It is regarded as an internal fragment of plasminogen containing the first 4 Kringle structures. In vitro, angiostatin specifically inhibits endothe-lial cell proliferation. In vivo, it inhibits angiogenesis and suppresses the growth of such primary tumors as Lewis lung carcinoma and hemangioendotheliolma in mice. | Hong Yang Yin Wu Yuxin Li Tonghui Ma Baiqu Huang | 2001 | Chinese Science Bulletin2001，46，6： | 0 |
| 12 | Microdissection of chromosome 7B of common wheat and cloning of low-copy specific DNA sequences显示文摘The 7B chromosome of common wheat was microdissected from pollen mother cells of the 7B monosomic line of common wheat cv. Chinese Spring (CS). After proteinase K and DNA topoisomerase Ⅰtreatments, the isolated chromosomes were subjected to 1—3 rounds of DOPPCR amplification, which produced continuous DNA fragments ranging from 150 to 700 bp. Genomic Southern hybridization confirmed that the PCR products were originated from the wheat genome. Cloning of portion ( > 200 bp) of the 3rd round DOP-PCR products (50 μL) could generate about 20 000 recombinant clones. Characterization of 50 randomly chosen clones indicated that 21 clones produced discrete PCR products with the size of 240—600 bp. Dot-blot hybridization showed that among the 21 clones, 11 (~ 55%) were of low-copy nature while 10 (~45%) were repetitive. Southern hybridization with the complete set of the CS 'nullisomic-tetrasomic (NT)' lines demonstrated that all the 6 low-copy clones were specific to either chromosome 7B or the 7th | Bao Liu Junkang Rong Yingshan Dong Fangpu Han Zhenlan Liu Mengyuan He Baiqu Huang Shui Hao | 1999 | Chinese Science Bulletin1999，44，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员