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| 1 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 2 | Burden of Clostridium difficile infection between 2010 and 2013:Trends and outcomes from an academic center in Eastern Europe显示文摘AIM:To analyze the incidence and possible risk factors in hospitalized patients treated with Clostridium difficile infection(CDI).METHODS:A total of 11751 patients were admitted to our clinic between 1 January 2010 and 1 May2013.Two hundred and forty-seven inpatients were prospectively diagnosed with CDI.For the risk analysis a 1:3 matching was used.Data of 732 patients matched for age,sex,and inpatient care period and unit were compared to those of the CDI population.Inpatient records were collected from an electronic hospital database and comprehensively reviewed.RESULTS:Incidence of CDI was 21.0/1000 admissions(2.1%of all-cause hospitalizations and 4.45%of total inpatient days).The incidence of severe CDI was 12.6%(2.63/1000 of all-cause hospitalizations).Distribution of CDI cases was different according to the unit type,with highest incidence rates in hematology,gastroenterology and nephrology units(32.9,25 and24.6/1000 admissions,respectively) and lowest rates in 1.4%(33/2312) in endocrinology and general internal medicine(14.2 and 16.9/1000 admissions)units.Recurrence of CDI was 11.3%within 12 wk after discharge.Duration of hospital stay was longer in patients with CDI compared to controls(17.6 ± 10.8d vs 12.4 ± 7.71 d).CDI accounted for 6.3%of allinpatient deaths,and 30-d mortality rate was 21.9%(54/247 cases).Risk factors for CDI were antibiotic therapy[including third-generation cephalosporins or fluoroquinolones,odds ratio(OR) = 4.559;P < 0.001],use of proton pump inhibitors(OR = 2.082,P< 0.001),previous hospitaiization within 12 mo(OR = 3.167,P < 0.001),previous CDI(OR = 15.32;P < 0.001),while presence of diabetes mellitus was associated with a decreased risk for CDI(OR = 0.484;P< 0.001).Treatment of recurrent cases was significantly different from primary infections with more frequent use of vancomycin alone or in combination(P < 0.001),and antibiotic therapy duration was longer(P < 0.02).Severity,mortality and outcome of primary infections and relapsing cases did not significantly differ.CONCLUSION:CDI was accounted for significant burden with longer hospitaiization and adverse outcomes.Antibiotic,PPI therapy and previous hospitaiization or CDI were risk factors for CDI. | Zsuzsanna Kurti Barbara D Lovasz Michael D Mandel Zoltan Csima Petra A Golovics Bence D Csako Anna Mohas Lorant Gnczi Krisztina B Gecse Lajos S Kiss Miklos Szathmari Peter L Lakatos | 2015 | World Journal of Gastroenterology2015，21，21： | 6 |
| 3 | Mechanisms involved in the inhibition of myoblast proliferation and differentiation by myostatin显示文摘 | Dominique Joulia Henri Bernardi Véronique Garandel Fanjaniriana Rabenoelina Barbara Vernus Gérard Cabello | 2003 | Experimental Cell Research2003，，2： | 3 |
| 4 | 血糖控制良好的2型糖尿病患者血清VEGF-A及其受体VEGFR1和VEGFR2含量检测(英文)显示文摘研究目的:探讨血糖控制良好的2型糖尿病患者血管内皮生长因子-A(VEGF-A)及其受体VEGFR1和VEGFR2含量及其临床意义。研究方法:本文以31例血糖得到良好控制且没有明显大血管或微血管病变的2型糖尿病患者作为研究对象(实验组),30位健康志愿者为对照组,同期检测患者及健康志愿者的空腹血糖、血脂、糖化血红蛋白水平,及血清VEGF-A、VEGFR1和VEGFR2含量,并加以比较和统计学分析。重要结论:研究结果表明,血糖控制良好的2型糖尿病患者血清中的VEGF-A及其受体VEGFR1和VEGFR2的含量和健康志愿者基本一致,两者无统计学意义,这可能显示血糖水平的合理控制能延缓血管并发症的产生。同时,2型糖尿病患者的血清VEGFR2含量和高密度脂蛋白胆固醇水平之间存在负相关,而血清VEGF-A、VEGFR2含量和甘油三脂水平之间存在正相关,这也表明糖尿病患者的血脂紊乱可能参与了血管生成的调节。 | Barbara RUSZKOWSKA-CIASTEK Alina SOKUP Maciej W. SOCHA Zofia RUPRECHT Lidia HALAS Barbara GóRALCZYK Krzysztof GóRALCZYK Grazyna GADOMSKA Danuta ROSC | 2014 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2014，15，6： | 2 |
| 5 | Technical outcomes of sentinel-lymph-node resection and conventional axillary-lymph-node dissection in patients with clinically node-negative breast cancer: results from the NSABP B-32 randomised phase III trial显示文摘 | David N Krag Stewart J Anderson Thomas B Julian Ann M Brown Seth P Harlow Takamaru Ashikaga Donald L Weaver Barbara J Miller Lynne M Jalovec Thomas G Frazier R Dirk Noyes André Robidoux Hugh MC Scarth Denise M Mammolito David R McCready Eleftherios P Mamo | 2007 | Lancet Oncology2007，，10： | 2 |
| 6 | Intestinal inflammation and activation of sensory nerve pathways:a functional and morphological study in the nematode infected rat 显示文摘 | Giorgio R Barbara G Blennerhassett P | 2001 | Gut2001，49，： | 2 |
| 7 | Kinetics of the hydrolysis of Atlantic salmon (Salmo salar) muscle proteins by alkaline proteases and a visceral serine protease mixture显示文摘 | Hordur G K Barbara A R | 2000 | Process Biochem2000，36，12： | 1 |
| 8 | Glucagon-like peptide 2:A nutrientresponsive gut growth factor显示文摘 | Douglas G B Yvette P Barbara S | 2001 | The Journal of Nutrition Bethesda2001，131，3： | 1 |
| 9 | Intestinal microbiota in functional bowel disorders : a Rome foundation report显示文摘 | Simren M Barbara G Flint H J | 2013 | Gut2013，62，1： | 1 |
| 10 | The hnpact of the Alliance on the Partners: a Look at Cause- Brand Alliances 显示文摘 | Lafferty Barbara A Gold Smith Ronald E Huh G Tomas M | 2004 | Psychology & Marketing2004，21，7： | 1 |
| 11 | Uranium reduction by desulfovibrio desulfuricans strain G20 and a cytochrome c3 mutant 显示文摘 | Rayford B P Darren M G Barbara J R | 2002 | Applied and Environmental Microbiology2002，68，6： | 1 |
| 12 | Elderly patients on dialysis: Epidemiology of an epidemic显示文摘 | GIUSEPPE P MARIO S BARBARA P G | 1996 | Nephrol Dail Transplant1996，11，9： | 1 |
| 13 | Development of an immunoassay-based lateral flow dipstick for the rapid detection of aflatoxin B1 in pig feed显示文摘 | BARBARA S D SARAH M D G S LIBERTY S | 2005 | Agric Food Chem2005，53，： | 1 |
| 14 | Visual function in children with hemiplegia in the first years of life显示文摘 | Andrea G Barbara F Eugenio M | 2001 | Dev Med Child Neurol2001，43，： | 1 |
| 15 | Multidrug Resistance Proteins\sMRP3,MRP1,and MRP2 in Lung Cancer:Correlation of Protein Levels\swith Drug Response and Messenger RNA Levels显示文摘 | Leah C Barbara G Susan P | 2001 | Clinical Cance\sResearch2001，7，： | 1 |
| 16 | Putative inflammatory and im munological mechanism in functional bowel disorders显示文摘 | Collins SM Vallance B Barbara G | 1999 | Bailliere''s Clin Gastroenterol1999，13，： | 1 |
| 17 | Norovirus gastroenteritis 显示文摘 | Cremon C De Giorgio R Barbara G | 2010 | N Engl J Med2010，362，55： | 1 |
| 18 | Po stoperative analgesia with remifentanil in patients undergoing cardiac surgery显示文摘 | BARBARA S HERBERT K GEORG G | 2005 | Anest h Analg2005，100，6： | 1 |
| 19 | Kinetics of the hydrolysis of Atlantic salmon muscle proteins by alkaline protease and a visceral serine protease mixture显示文摘 | Hordur G Kristinsson Barbara A Rasco | 2000 | Process biochemistry2000，36，： | 1 |
| 20 | Intestinal inflammation and activation of sensory nerve pathways: a functional and morphological study in the nematode infected rat显示文摘 | Giorgio Rde Barbara G Blennerhassett P | 2001 | Gut2001，49，： | 1 |
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