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| 1 | Peptidergic innervation of human esophageal and cardiac carcinoma显示文摘AIM: To investigate the distribution of neuropeptideimmunoreactive nerve fibers in esophageal and cardiac carcinoma as well as their relationship with tumor cells so as to explore if there is nerve innervation in esophageal and cardiac carcinoma.METHODS: Esophageal and cardiac carcinoma specimens were collected from surgical operation. One part of them were fixed immediately with 4 % paraformaldehyde and then cut with a cryostat into 40-pm-thick sections to perform immunohistochemical analysis. Antibodies of ten kinds of neuropeptide including calcitonin gene-related peptide (CGRP), galanin (GAL), substance P (SP), etc. were used for immunostaining of nerve fibers. The other part of the tumor specimens were cut into little blocks (1 mm3) and cocultured with chick embryo dorsal root ganglia (DRG) to investigate if the tumor blocks could induce the neurons of DRG to extend processes, so as to probe into the possiblereasons for the nerve fibers growing into tumors. RESULTS: Substantial amounts of neuropeptide including GAL-, NPY-, SP-immunoreactive nerve bundles and scattered nerve fibers were distributed in esophageal and cardiac carcinomas. The scattered nerve fibers waved their way among tumor cells and contacted with tumor cells closely. Some of them even encircled tumor cells. There were many varicosities aligned on the nerve fibers like beads. They were also closely related to tumor cells. In the co-culture group, about 63 %and 67 % of DRG co-cultured with esophageal and cardiac tumor blocks respectively extended enormous processes,especially on the side adjacent to the tumor, whereas in the control group (without tumor blocks), no processes grew out.CONCLUSION: Esophageal and cardiac carcinomas may be innervated by peptidergic nerve fibers, and they can induce neurons of DRG to extend processes in vitro. | Shuang-Hong Lü Yan Zhou Hai-Ping Que Shao-Jun Liu Department of Neurobiology, Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China | 2003 | World Journal of Gastroenterology2003，9，3： | 9 |
| 2 | Characterization of glucose-6-phosphate dehydrogenase deficiency and identification of a novel haplotype 487G>A/IVS5-612(G>C) in the Achang population of southwestern China显示文摘The prevalence of glucose-6-phosphate dehydrogenase (G6PD) deficiency and its gene mutations were studied in the Achang population from Lianghe County in Southwestern China. We found that 7.31% (19 of 260) males and 4.35% (10 of 230) females had G6PD deficiency. The molecular analysis of G6PD gene exons 2―13 was performed by a PCR-DHPLC-Sequencing or PCR-Sequencing. Sixteen inde-pendent subjects with G6PD Mahidol (487G>A) and the new polymorphism IVS5-612 (G>C), which combined into a novel haplotype, were identified accounting for 84.2% (16/19). And 100% Achang G6PD Mahidol were linked to the IVS5-612 C. The percentage of G6PD Mahidol in the Achang group is close to that in the Myanmar population (91.3% 73/80), which implies that there are some gene flows between Achang and Myanmar populations. Interestingly, G6PD Canton (1376G>T) and G6PD Kaiping (1388G>A), which were the most common G6PD variants from other ethnic groups in China, were not found in this Achang group, suggesting that there are different G6PD mutation profiles in the Achang group and other ethnic groups in China. Our findings appear to be the first documented report on the G6PD genetics of the AChang people, which will provide important clues to the Achang ethnic group origin and will help prevention and treatment of malaria in this area. | YANG YinFeng, ZHU YueChun, LI DanYi, LI ZhiGang, Lü HuiRu, WU Jing, TANG Jing & TONG ShuFen Department of Biochemistry, Faculty of Basic Medicine, Kunming University of Medical Sciences, Kunming 650031, China These authors contributed equally to this work | 2007 | Science China(Life Sciences)2007，50，4： | 6 |
| 3 | Post - stroke depression显示文摘 | Zavoreo L Basic - Kes V Bosnar - Puretic M | 2000 | Acta Clin Croat2000，48，3： | 1 |
| 4 | Determination of the different states of mercury in seawater near the Vlora and Durres Bays显示文摘 | Perring L Basic Dvorzak M | 2002 | Anal Bioanal Chem2002，374，6： | 1 |
| 5 | Variant alleles, triallelic patterns, and point mutations observed in nuclear short tandem repeat typing of populations in Bosnia and Serbia 显示文摘 | Huel R L Basic L Madacki-Todorovi6 K | 2007 | Croat Med J2007，48，4： | 1 |
| 6 | Occurrence, distribution and control of papaya mealybug, Paracoccus marginatus (Hemiptera : Pseudococcidae), an invasive alien pest in Sfi Lanka 显示文摘 | Hemiptera: of Basic & Galanihe L D Jayasundera M U P Vithana A et ol | 2011 | Tropical Agricultural Research & Extension2011，13，3： | 1 |
| 7 | Determination of total tin in canned food using inductively coupled plasma atomic emission spectroscopy 显示文摘 | PERRING L BASIC DVORZAK M | 2002 | Analytical and Bioanalytieal Chemistry2002，374，2： | 1 |
| 8 | Immunohistochemical evaluation of Cyclin D1 in breast cancer显示文摘 | Bilalovic N Vranic S Basic H et a l | | 0，，03： | 1 |
| 9 | 显示文摘 | Oxvig C Sand O Kristensen T Gleich GJ & Sottrup-- Jensen L Circulating human pregnancy-associated plasma protein-A is disulfide-bridged to the proform of eosinophil major basic protein | 1993 | Journal of Biological Chemistry1993，268，12： | 1 |
| 10 | Surface electrocardiogram and action potential in mice lacking urea transporter UT-B显示文摘UT-B is a urea transporter protein expressed in the kidney and in many non-renal tissues including erythrocytes, brain, heart, bladder and the testis. The objective of this study was to determine the phenotype of UT-B deletion in the heart. UT-B expression in the heart was studied in wild-type mice vs UT-B null mice by utilizing RT-PCR and Western blot. A surface electrocardiogram (ECG) recording (lead II) was measured in wild-type mice and UT-B null mice at the ages of 6, 16 and 52 weeks. For the action potential recording, the ventricular myocytes of 16 w mice were isolated and recorded by floating microelectrode method. The sodium current was recorded by the patch clamp technique. RT-PCR and Western blot showed the UT-B expression in the heart of wild-type mice. No UT-B transcript and protein was found in UT-B null mice. The ECG recording showed that the P-R interval was significantly prolonged in UT-B null mice ((43.5 ± 4.2), (45.5 ± 6.9) and (43.8 ± 7.6) ms at ages of 6, 16 and 52 weeks) vs wild-type mice ((38.6 ± 2.9), (38.7 ± 5.6) and (38.2 ± 7.3) ms, P<0.05). The atrial ventricular heart block type II and III only appeared in the aging UT-B null mice (52 w old). The amplitude of action potential and Vmax decreased significantly in UT-B null mice ((92.17 ± 10.56) and (101.89 ± 9.54) mV/s) vs those in wild-type mice (vs (110.51 ± 10.38) and (109.53 ± 10.64) mV/s, P<0.05). The action potential duration at 50% and 90% (APD50 and APD90) was significantly prolonged in UT-B null mice ((123.83 ± 11.17) and (195.43 ± 16.41) ms) vs that in wild-type mice ((108.27 ± 10.85) and (171.00 ± 15.53) ms, P<0.05). The maximal sodium current decreased significantly in UT-B null mice (-8.80 ± 0.92) nA vs that in wild-type mice ((-5.98 ± 1.07) nA, P<0.05). These results provide the first evidence that UT-B deletion causes progressive heart block in mice. | MENG Yan1, ZHAO ChunYan2, ZHANG XueXin2, ZHAO HuaShan1, GUO LiRong1, Lü Bin1, ZHAO XueJian1& YANG BaoXue1,3 1 Department of Pathophysiology, School of Basic Medicine, Jilin University, Changchun 130021, China 2 Department of Physiology, School of Basic Medicine, Jilin University, Changchun 130021, China 3 Department of Medicine, University of California, San Francisco CA 94143, USA | 2009 | Science China(Life Sciences)2009，52，5： | 1 |
| 11 | A quantification model for apoptosis in mouse embryos in the early stage of fetation显示文摘Apoptosis is the most important inducement and modulator for embryos in the early stage of fetation, i.e. after the 8-cell stage, mostly the morula and blastula stage, to proceed to the stage of nonlinear development. Using a two-photon laser scanning microscopy (TPLSM) system, we obtained 3-dimensional (3D) fluorescent images of preimplantation mouse embryos. A model for quantification was established. The statistical results for the spatial location of apoptosis bodies in embryos was obtained following image processing, as well as investigation of the kinetics of apoptosis. It was found that most (70%) apoptosis occurred in the trophectoderm, and the departure between the centroid and geometric center of embryos had a step transition when embryos developed into the 32-cell stage, which was consistent with the theoretical prediction that the blastocele would induce a symmetry break of the distribution of cells in embryos. | WANG PengFei1, FU JianHua1, MA WanYun1, CHEN DieYan1, Lü DanYu2 & BAI WenJia3 1 Key Laboratory for Atomic and Molecular Nanosciences of Education Ministry, Department of Physics, Tsinghua University, Beijing 100084, China 2 Department of Anatomy & Histology and Embryology, School of Basic Medical Sciences Peking University Health Science Center, Beijing 100083, China 3 Department of Automation, Tsinghua University, Beijing 100084, China | 2009 | Science China(Life Sciences)2009，52，10： | 0 |
| 12 | Local connectivity of Julia sets for a family of biquadratic polynomials显示文摘 | Jing L~(1+) Wei-yuan QIU~2 ~1 Department of Basic Education,Shanghai Maritime University,Shanghai 200000,China ~2 Department of Mathematics,Fudan University,Shanghai 200433,China | 2007 | Science China Mathematics2007，50，10： | 0 |
| 13 | Nerve-heart interplays recorded in rabbit hearts in vivo显示文摘Neurotransmitter release from presynaptic cells can be recorded by postsynaptic potentials/currents in central nerve system. However, little is known about how to | Lei Qian1,2, Gu Jing-Li 1,3, Lü Jin 1,3, Mu Yu 1,2, Xiu Yun1,3, Xing Jiang Kang 1, Guo Shu 1,3, Xu Hua-Dong1, Zhang Xiao-Yu 1, Zhang Claire Xi1, Cao Ji-Min 3, Li Li-Huan2, Zhou Zhuan 1 1Institute of Molecular Medicine, Peking University, Beijing 100871, China 2Fuwai Hospital, and 3Institute of Basic Medical Science, Peking Union Medical College, Beijing 100005,China | 2009 | 生物物理学报2009，25，S1： | 0 |
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