2篇
您的检索式：作者名="Baskar Balakrishnan"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Purification,characterization and production optimization of a vibriocin produced by mangrove associated vibrio parahaemolyticus显示文摘Objective:To identify a potential bacterium which produces antimicrobial peptide(vibriocin),and its purification,characterization and production optimization.The bacteria subjected in the study were isolated from a highly competitive ecological niche of mangrove ecosystem.Methods:The bacterium was characterized by phenotype besides 16S rRNA gene sequence analysis.The antibacterial activity was recognised by using agar well diffusion method.The vibriocin was purified using ammonium sulphate precipitation,butanol extraction,gel filtration chromatography,ion-exchange chromatography and subsequently,by HPLC.Molecular weight of the substance identified in SDS-PAGE.Production optimization performed according to Taguchi's mathematical model using 6 different nutritional parameters as variables.Results:The objective bacterium was identified as Vibrio parahaemolyticm.The vibriocin showed 18 KDa of molecular mass with mono peptide in nature and highest activity against pathogenic Vibrio harveyi.The peptide act stable in a wide range of pH,temperature.UV radiation,solvents and chemicals utilized.An overall^20%of vibriocin production was improved,and was noticed that NaCl and agitation speed played a vital role in secretion of vibriocin.Conclusion:The vibriocin identified here would he an effective alternative for chemically synthesized drugs for the management of Vibrio infections in mariculture industry. | Baskar Balakrishnan Jayappriyan Kothilmozhian Ranishree Sathish Thadikamala Prabakaran Panchatcharam | 2014 | Asian Pacific Journal of Tropical Biomedicine2014，4，4： | 5 |
| 2 | Biosorption of an azo dye from aqueous solutions onto Spirogyra显示文摘 | Sivarajasekar N Baskar R Balakrishnan V | 2009 | J Univ Chem Technol Metall2009，44，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员