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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Do Recessions Permanently Change Output? 显示文摘 | BEAUDRY P KOOP G | 1993 | Journal of Monetary Economics1993，31，： | 1 |
| 3 | Membrane technology for direct osmosis concentration of fruit juices 显示文摘 | BEAUDRY E G LAMPI K A | 1990 | J Food Technol1990，44，： | 1 |
| 4 | Forwardosmosis treatment of drilling mud and fracturing wastewater from oil and gas operations显示文摘 | Appleton E W Beaudry E G Xu P | 2013 | Desalination2013，，312： | 1 |
| 5 | The monkey and human uridine diphosphate glucuronosyltransferase UGT1A9,expressed in steroid target tissues,are estrogen-conjugating enzymes显示文摘 | Albert C Vallee M Beaudry G | 1999 | Endocrinology1999，140，： | 1 |
| 6 | Identification of PCA3 (DD3) in prostatic carcinoma by in situ hybridization 显示文摘 | POPA I FRADET Y Beaudry G | 2007 | Mod Pathol2007，20，11： | 1 |
| 7 | Clozapine and dopamine D3 receptor antisense reduce cocaine-and amphetamine regulated transcript expression in the rat nucleus accumbens shell显示文摘 | Beaudry G Zekki H Rouillard C | 2004 | Synapse2004，51，4： | 1 |
| 8 | The sweet spot of forward osmosis: Treatment of produced water, drilling wastewater, and other complex and difficult liq- uid streams 显示文摘 | Coday B D Xu P Beaudry E G etal | 2014 | Desalination2014，333，1： | 1 |
| 9 | Impact of antipsychotic drug administration on the expression of nuclear receptors in the neocortex andstriatum of the rat brain显示文摘 | Langlois MC Beaudry G Zekki H | 2001 | Neuroscience2001，106，1： | 1 |
| 10 | Differential effects of vascular endothelial growth factor receptor-2 inhibitor ZD6474 on circulating endothelial progenitors and mature circulating endothelial cells:implications for use as a surrogate marker of antiangiogenic activity显示文摘 | BEAUDRY P FORCE J NAUMOV G N | 2005 | Clin Cancer Res2005，11，9： | 1 |
| 11 | Differential effects of vascular endothelial growth factor receptor-2 inhibitor ZD6474 on circulating endothelial progenitors and mature circulating endothelial ceils: implications for use as a surrogate marker of antiangiogenic activity 显示文摘 | BEAUDRY P FORCE J NAUMOV G N | 2005 | Clin Cancer Res2005，11，9： | 1 |
| 12 | Comparison of histopathology and RT-qPCR amplification of guanylyl eyelase C for detection of colon cancer metastases in lymph nodes显示文摘 | Haince JF Houde M Beaudry G | 2010 | J Clin Pathol2010，63，6： | 1 |
| 13 | Impact of antipsychotic drug administration on the expression of nuclear receptors in the neocortex and striatum of the rat brain显示文摘 | Langlois MC Beaudry G Zekki H | 2001 | Neuroscience2001，106，1： | 1 |
| 14 | The sweet spot of forward osmosis: treatment of produced water, drilling wastewater, and other complex and difficult liquid streams 显示文摘 | Coday R D Xu P Beaudry E G | 2014 | Desalination2014，，： | 1 |
| 15 | Contrasting Patterns and Cellular Specificity of Transcriptional Regulation of the Nuclear Receptor Nerve Growth Factor‐Inducible B by Haloperidol and Clozapine in the Rat Forebrain显示文摘 | Beaudry G Langlois MC Weppe I | 2000 | J Neurochem2000，75，4： | 1 |
| 16 | Serial analysis of gene expression in non-small cell lung cancer显示文摘 | HIBI K LIU Q BEAUDRY G A | 1998 | Cancer Res1998，58，24： | 1 |
| 17 | Membrane contactorprocesses for wastewater reclamation in space I : Direct osmoticconcentration as pretreatment for reverse osmosis 显示文摘 | Cath T Y Gormly S Beaudry E G | 2005 | Journal ofMembrane Science2005，257，1： | 1 |
| 18 | Effect of four drying methods on the quality of osmotically dehydrated cranberries 显示文摘 | Beaudry C Raghavan G S Ratti C et at | 2004 | Drying Technology2004，22，3： | 1 |
| 19 | Differential effects of vascular endothelial growth factor receptor- 2 inhibitor ZD6474 on circulating endothelial progenitors and mature circulating endothelial cells: implications for use as a surrogate marker of antiangiogenic activity 显示文摘 | Beaudry P Force J Naumov G N | 2005 | Clin Cancer Ras2005，11，9： | 1 |
| 20 | Application of forward osmosis in pretreatment of seawater for small reverse osmosis desalination units 显示文摘 | Bamaga O A Yokochi A Beaudry E G | 2009 | Desalination and Water Treatment2009，5，13： | 1 |
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