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| 1 | Effects of live yeast on differential genetic and functional attributes of rumen microbiota in beef cattle显示文摘Several studies have evaluated the effects of live yeast supplementation on rumen microbial population;however,its effect on differential microbial genes and their functional potential has not been described. Thus, this study applied shotgun metagenomic sequencing to evaluate the effects of live yeast supplementation on genetic and functional potential of the rumen microbiota in beef cattle. Eight rumen-cannulated Holstein steers were randomly assigned to two treatments in a cross-over design with two 25-day experimental periods and a 10-day wash-out between the two periods. The steers were housed in individual pens and fed 50% concentrate-mix and 50% red clover/orchard hay ad libitum. Treatments were(1) control(CON;basal diet without additive) and(2) yeast(YEA;basal diet plus 15 g/d of live yeast product). Rumen fluid samples were collected at 3, 6, and 9 h after feeding on the last d of each period. Sequencing was done on an Illumina Hi Seq 2500 platform. Dietary yeast supplementation increased the relative abundance of carbohydrate-fermenting bacteria(such as Ruminococcus albus, R.champanellensis, R. bromii, and R. obeum) and lactate-utilizing bacteria(such as Megasphaera elsdenii, Desulfovibrio desulfuricans, and D. vulgaris). A total of 154 differentially abundant genes(DEGs) were obtained(false discovery rate < 0.01). Kyoto Encyclopedia of Genes and Genomes(KEGG) annotation analysis of the DEGs revealed that 10 pathways, including amino sugar and nucleotide sugar metabolism, oxidative phosphorylation, lipopolysaccharide biosynthesis, pantothenate and coenzyme A biosynthesis, glutathione metabolism, beta-alanine metabolism,polyketide sugar unit biosynthesis, protein export, ribosome, and bacterial secretory system, were enriched in steers fed YEA. Annotation analysis of the DEGs in the carbohydrate-active enzymes(CAZy) database revealed that the abundance of genes coding for enzymes belonging to glycoside hydrolases, glycosyltransferases, and carbohydrate binding modules were enriched in steers fed YEA. These results confirm the effectiveness of a live S. cerevisiae product for improving rumen function in beef steers by increasing the abundance of cellulolytic bacteria, lactic acid-utilizing bacteria, and carbohydrate-active enzymes in the rumen. | Ibukun M.Ogunade Jerusha Lay Kenneth Andries Christina J.Mc Manus Frederick Bebe | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 11 |
| 2 | Physico-chemical characteristics of corrosion scales in old iron pipes 显示文摘 | SARIN P SNOEYINK V L BEBEE J | 2001 | Water Research2001，35，12： | 1 |
| 3 | Dependence of catalytic rates on catalyst work function显示文摘 | Vayenas C G Bebelis S Ladas S | 1990 | Nature1990，343，： | 1 |
| 4 | Educationg leaders in hospital management:a new model in Sub-Saharan Africa显示文摘 | Sosena Kebede Yigeremu A bebe | | 0，，01： | 1 |
| 5 | Iron release fromcorroded iron pipes in drinking water distribution sys-tems :effect of dissolved oxygen显示文摘 | Sarin P Soneyink V L Bebee J | 2004 | Water Res2004，38，5： | 1 |
| 6 | Evaluation of the efficiency of alternative two-tier nucleus breeding systems designed to improve meat sheep in Kenya显示文摘 | GICHEHA M G KOSGEY I S BEBE B O | 2006 | Joural of Animal Breeding and Genetics2006，123，4： | 1 |
| 7 | Physicochemical characteristics of corrosion scales in old iron pipes显示文摘 | Sarin P Snoeyink V L Bebee J | 2001 | Wat Res2001，35，12： | 1 |
| 8 | Physico-chemical characteristics of corrosion scales in old iron pipes显示文摘 | Sarin P Snoeyink V.L Bebee J Kriven W.M | | 0，，12： | 1 |
| 9 | Physico - chemical characteristics of corrosion scales in old iron pipes 显示文摘 | SARIN P SNOEYINK V L BEBEE J | 2001 | Wat Res2001，35，12： | 1 |
| 10 | Conserved sequences in the final intron of MDM2 are essential for the regulation of alternative splicing of MDM2 in response to stress显示文摘 | Singh RK Tapia-Santos A Bebee TW | 2009 | Experimental Cell Research2009，315，19： | 1 |
| 11 | Growth performance of beef calves fed maize or sweet sorghum Silages treated with urea or ammonium hydroxide显示文摘 | BEBE F N FELIX A | 1996 | Tropieal Agriculture1996，73，1： | 1 |
| 12 | Benzodiazepine actions mediated by specific gamma aminobutyrie acid A receptor subtypes显示文摘 | Rudolph U Crestani F Bebe D | 1999 | Nature1999，401，： | 1 |
| 13 | Mechanism of release of iron from corroded iron/steel pipes in water distribution systems显示文摘 | Bebee J Beckett M A | 2001 | Corrosion & Material2001，26，3： | 1 |
| 14 | Compact duplexer-polarizer with semicircular waveguide显示文摘 | Bebe R Barchat P | | 0，，08： | 1 |
| 15 | Field evaluation of somaclonal variation in rye lines differing in telomeric heterochromatin 显示文摘 | Kaltsikes P J Karp A | 1993 | J Genetic Breed1993，47，： | 1 |
| 16 | Benzodiazepine actions mediated by specificgamma-aminobutyric acid A receptor subtypes显示文摘 | Rudolph U Crestani F Bebe D | 1999 | Nature1999，401，： | 1 |
| 17 | Physico-chemical chracteristics of corrosion scales in old iron pipes 显示文摘 | Sarin P Snoeyink V L Bebee J | 2001 | Water Res2001，35，12： | 1 |
| 18 | Physico-chemical characteristics of corrosion scales in old iron pipes显示文摘 | Sarin P Snoeyink V L Bebee J | 2001 | Water Res2001，35，12： | 1 |
| 19 | Mechanism of Release of Iron from Corroded Iron/Steel Pipes in Water Distribution Sys- tems显示文摘 | SARIN P BEBEE J BECKETT M | 2001 | Corrosion&Materials2001，26，3： | 1 |
| 20 | Electrochemical promo- tion of heterogeneous catalysis显示文摘 | VAYENAS C G BEBELIS S | 1999 | Catal Today1999，51，34： | 1 |
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