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| 1 | Site-directed mutagenesis identified the key active site residues of alcohol acyltransferase PpAAT1 responsible for aroma biosynthesis in peach fruits显示文摘The aroma of peach fruit is predominantly determined by the accumulation of γ-decalactone and ester compounds.A previous study showed that the biosynthesis of these aroma compounds in peach fruit is catalyzed by PpAAT1,an alcohol acyltransferase.In this work,we investigated the key active site residues responsible for γ-decalactone and ester biosynthesis.A total of 14 candidate amino acid residues possibly involved in internal esterification and 9 candidate amino acid residues possibly involved in esterification of PpAAT1 were assessed via site-directed mutagenesis.Analyses of the in vitro enzyme activities of PpAAT1 and its site-directed mutant proteins(PpAAT1-SMs)with different amino acid residue mutations as well as the contents of γ-decalactone in transgenic tobacco leaves and peach fruits transiently expressing PpAAT1 and PpAAT1-SMs revealed that site-directed mutation of H165 in the conserved HxxxD motif led to lost enzymatic activity of PpAAT1 in both internal esterification and its reactions,whereas mutation of the key amino acid residue D376 led to the total loss ofγ-decalactone biosynthesis activity of PpAAT1.Mutations of 9 and 7 other amino acid residues also dramatically affected the enzymatic activity of PpAAT1 in the internal esterification and esterification reactions,respectively.Our findings provide a biochemical foundation for the mechanical biosynthesis ofγ-decalactone and ester compounds catalyzed by PpAAT1 in peach fruits,which could be used to guide the molecular breeding of new peach species with more favorable aromas for consumers. | Zhi-Zhong Song Bin Peng Zi-Xia Gu Mei-Ling Tang Bei Li Mei-Xia Liang Li-Min Wang Xiao-Tong Guo Jian-Ping Wang Yu-Fen Sha Hong-Xia Zhang | 2021 | Horticulture Research2021，8，1： | 3 |
| 2 | Increase of the plasma α‐synuclein levels in patients with multiple system atrophy显示文摘 | Zhan‐Fang Sun Xiao‐Shuang Xiang Zhao Chen Li Zhang Bei‐Sha Tang Kun Xia Hong Jiang | 2014 | Mov Disord2014，，： | 1 |
| 3 | Construction and immunogencity of a Delta apxIC Delta apxIIC double mutant of Actinobacillus pleuropneumoniae serovar 1显示文摘 | Lin L Bei W Sha Y | 2007 | FEMS Microbiol Lett2007，274，1： | 1 |
| 4 | A gross deletion of the PAX3 gene in a large Chinese family with Waardenburg syndrome type Ⅰ显示文摘Waardenburg syndrome(WS)is an autosomal-dominant neurocristopathy characterized by sensorineural hearing loss(SNHL)and pigmentary abnormalities of the iris,hair,and skin.It is responsible for approximately 3%of congenital hearing loss[1].WS is thought to be fully penetrant when considering at least one clinical feature,but the penetrance of each feature is not complete[2].Four types of WS have been classifed based on the clinical manifestations.WS1 is one of the most common forms of WS.To confrm the diagnosis of WS1,at least two major or one major plus two minors of the Waardenburg consortium criteria must be present.In brief,major criteria include congenital sensorineural hearing loss,pigmentary disturbances of the iris,hair hypopigmentation,dystopia canthorum(average W index of afected family members is greater than 1.95)and afected frst-degree relatives.Minor criteria include skin hypopigmentation,synophyrys or medial eyebrow fare,broad and high nasal root,hypoplasia of alae nasi,and premature graying of hair[3].High phenotypic heterogeneity has been observed in WS1,even in the same family[4]. | Shu‑Zhi Yang Lei Hou Xin Qi Guo‑Jian Wang Sha‑Sha Huang Shan‑Shan Zhang Bang‑Qing Huang Ying Yang Bei‑Cheng Li Shuo Liu Pu Dai Yu Su | 2023 | World Journal of Pediatrics2023，19，12： | 0 |
| 5 | Event-Driven SOA Based Integration Platform for Mold Making Industry显示文摘The platform consists of three layers: the management service layer administers the infrastructure of the whole platform; the integration service layer provides the core integration functions and the user service layer satisfies the enterprise integration demands. For the purpose of accommodating the characteristics of mold making companies, an event-driven enterprise service bus working as the heart of the platform is realized. A prototype system combining Web services, Business Process Execution Language (BPEL) and Web Ontology Language (OWL) is implemented. The clients and suppliers and the applications of mold making company are integrated in a loosely-coupled way through the services provided by the platform. Meanwhile the sharing of data and knowledge, and the coordination of business process are realized. By adopting the platform, a new approach is provided to solve the problem of design and manufacture integration for intra and inter mold making companies. | HU Sha LI Bei YANG Shuang-rong LI Jian-jun | 2009 | Computer Aided Drafting,Design and Manufacturing2009，19，1： | 0 |
| 6 | Executive summary of Chinese expert consensus for topical application of anti-microbial agents for lower respiratory tract infection in adults显示文摘In recent years,with the increased prevalence of multi-drug resistant(MDR)bacterial infection and chronic pulmonary infection,the topical application of anti-microbial agents,mainly inhaled antibiotics,has come back to clinical practice.Several formulations for nebulization and dry powder inhaler(DPI)have been approved for inhaled anti-infective therapy.Meanwhile,evidence and experience have been accumulated in the use of anti-microbial agents delivered via airway.However,the available studies in this field are heterogenous in the study population,drug delivery route and dosages.The efficacy and safety of inhaled anti-infective therapy in various types of lower respiratory tract infections(LRTIs)need to be evaluated.Moreover. | Jing Zhang Qijian Cheng Yi Huang Hong Fan Guoxiang Lai Xiangdong Mu Wei Sha Danyang She Ning Shen Xin Su Jinfu Xu Feng Ye Xinlun Tian Tiantuo Zhang Hua Zhou Youning Liu Lixian He Heping Xiao Bei He Yi Shi Xiangyan Zhang Bin Cao Jieming Qu on behalf of Pulmonary Infection Assembly of Chinese Thoracic Society | 2022 | Chinese Medical Journal2022，135，22： | 0 |
| 7 | Influence of modified light dispersion theory on fermion tunneling radiation in the Einstein–Maxwell–dilaton–axion black hole显示文摘Based on a light dispersion relationship derived from string theory and quantum gravitational theory,we make an accurate modification to the quantum tunneling radiation rate and black hole temperature at an event horizon in a stationary axial-symmetric Einstein–Maxwell–dilaton–axion black hole.We also analyze our new results and carry out some significant discussions.This work enriches the research content and methods of the frontiers of black hole physics. | Jie Zhang Zhie Liu Bei Sha Xia Tan Yuzhen Liu | 2020 | Communications in Theoretical Physics2020，72，4： | 0 |
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