维普期刊资源整合服务平台（V6.5）

维普中文期刊产品整合服务

1篇 您的检索式：作者名="Bencomo E."

选中清除导出查看参考文献查看引证文献引用追踪共1页首页上一页第1页下一页末页 /1 跳转

		题名	作者	年代	出处	被引量
	1	胰岛素样生长因子I降低黄体颗粒细胞凋亡,与胚胎破碎和妊娠率相关显示文摘Objective: To correlate apoptosis of cultured human granulosa- lutein cells (GL cells) with the outcome of IVF (embryo fragmentation and pregnancy rate) and to study the effect of insulin and insulin- like growth factor I (IGF- I) on apoptosis. Design: In vitro assays. Setting: University laboratory and private IVF center. Patient(s): Eighty- one women undergoing IVF. Intervention(s): Purified human GL cells from pooled follicles were cultured for 48 hours in serum- free media with or without insulin and IGF- I. Cumulus cells and mural GL cells were evaluated separately. Main Outcome Measure(s): Detection of apoptosis by using caspACE FITC- VAD- FMK, a fluorescent in situ marker for activated caspases; embryo fragmentation; and pregnancy. Result(s): Age younger than 38 years and successful pregnancy were associated with less apoptosis (33.0% ± 17.2% vs. 43.2% ± 18.0% and 30.2% ± 14.0% vs. 40.4% ± 19.5% , respectively). There was a linear correlation between embryo fragmentation and GL cell apoptosis. Insulin- like growth factor I decreased apoptosis in a dose- dependent fashion. A statistically significant effect (17% decrease) was reached at a dose of 10 nM. Insulin (10 nM) caused a small (8% ) decrease in apoptosis, but this effect did not reach statistical significance. Cumulus cells consistently had < 3% apoptosis. Conclusion(s): [1] Apoptosis of cultured GL cells may be associated with IVF outcome and ovarian reserve and [2] IGF- I decreases apoptosis of cultured GL cells.	Bencomo E. Pérez R. ArteagaM.-F. A. Palumbo 马超	2006	世界核心医学期刊文摘(妇产科学分册)2006，2，6：	0

返回顶部每页显示：

共1页首页上一页第1页下一页末页 /1 跳转

goolge学术搜索-维普

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费