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11篇 您的检索式:作者名="Benjamin CW"
    题名 作者 年代 出处 被引量
1Plasma lipidconcentrations during episodic occupational stress显示文摘McCann BS Benjamin GA Wilkinson CW 1999AnnBehav Med1999,21,:1
2Mesenchymal stem cells and collagen patches for anterior cruciate ligament repair显示文摘AIM: To investigate collagen patches seeded with mesenchymal stem cells(MSCs) and/or tenocytes(TCs) with regards to their suitability for anterior cruciate ligament(ACL) repair. METHODS: Dynamic intraligamentary stabilization utilizes a dynamic screw system to keep ACL remnants in place and promote biological healing, supplemented by collagen patches. How these scaffolds interact with cells and what type of benefit they provide has not yet been investigated in detail. Primary ACL-derived TCs and human bone marrow derived MSCs were seeded onto two different types of 3D collagen scaffolds, Chondro-Gide?(CG) and Novocart?(NC). Cells were seeded onto the scaffolds and cultured for 7 d either as a pure populations or as 'premix' containing a 1:1 ratio of TCs to MSCs. Additionally, as controls, cells were seeded in monolayers and in co-cultures on both sides of porous high-density membrane inserts(0.4 μm). We analyzed the patches by real time polymerase chain reaction, glycosaminoglycan(GAG), DNA and hydroxyproline(HYP) content. To determine cell spreading and adherence in the scaffolds microscopic imaging techniques, i.e., confocal laser scanning microscopy(c LSM) and scanning electron microscopy(SEM), were applied.RESULTS: CLSM and SEM imaging analysis confirmed cell adherence onto scaffolds. The metabolic cell activity revealed that patches promote adherence and proliferation of cells. The most dramatic increase in absolute metabolic cell activity was measured for CG samples seeded with tenocytes or a 1:1 cell premix. Analysis of DNA content and c LSM imaging also indicated MSCs were not proliferating as nicely as tenocytes on CG. The HYP to GAG ratio significantly changed for the premix group, resulting from a slightly lower GAG content, demonstrating that the cells are modifying the underlying matrix. Real-time quantitativepolymerase chain reaction data indicated that MSCs showed a trend of differentiation towards a more tenogenic-like phenotype after 7 d.CONCLUSION: CG and NC are both cyto-compatible with primary MSCs and TCs; TCs seemed to perform better on these collagen patches than MSCs.Benjamin Gantenbein Neha Gadhari Samantha CW Chan Sandro Kohl Sufian S Ahmad 2015World Journal of Stem Cells2015,7,2:1
3Plasma lipid coneentratinnsduring episodic occupational stress显示文摘McCann BS Benjamin GA Wilkinson CW 1999Ann Behav Med1999,21,2:1
4Increased case - fatalityrate associated with outbreaks of Neisseria meningitidis infection, com-pared with sporadic meningococcal disease, in the United States, 1994 -2002显示文摘Brooks R Woods CW Benjamin DK }r 200667m Infect Zfe2006,43,1:1
5Organization of the chondrocyte cytoskeleton and its response to changing mechanical conditions in organ culture显示文摘Durrant LA Archer CW Benjamin M 1999J Anat1999,194,:1
6Plasma lipid concentrations during episodic oecupational stress 显示文摘McCann BS Benjamin GA Wilkinson CW 1999Ann Behav Med1999,21,2:1
7A single-center double blind trial of Trasylol therapy in primary acute pancreatitis显示文摘Imrie CW Benjamin IS Ferguson JC et at 1978Br J Surg1978,65,5:1
8Caspases activation in MCF7 cells responding to etoposide treatment显示文摘Benjamin CW Hiebsch RR Jones DA 1998Mol Pharmacol1998,53,3:1
9A single centre double blind trial of Trasylol therapy in primary acute pancreatitis 显示文摘Imrie CW Benjamin IS Ferguson JC 1978Br J Surg1978,65,5:1
10A single-centre double- blind trial of Trasylol therapy in primary acute pancreatitis显示文摘Imrie CW Benjamin IS Ferguson JC 1978Br J Surg1978,65,5:1
11Outcomes of immed- iate breast reconstruction in patients undergoing single-stage sentinel lymph node biopsy and mastectomy 显示文摘Benjamin CW Edward AL Malcolm WM 2011Annals of Plastic Surgery2011,66,5:1
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