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| 1 | Alleviated mucosal and neuronal damage in a rat model of Crohn's disease显示文摘AIM:To establish a rat model suitable to investigate the repetitive relapsing inflammations(RRI)characteristic to Crohn’s disease.METHODS:Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid(TNBS).RRI were mimicked by repeating administrations of TNBS.Tissue samples were taken from control,once,twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation.The means of the ulcerated area were measured to evaluate the macroscopic mu-cosal damage.The density of myenteric neurons was determined on whole mounts by Hu C/Hu D immunohistochemistry.Heme oxygenase-1(HO-1)expression was evaluated by molecular biological techniques.RESULTS:TNBS-treated rats displayed severe colitis,but the mortality was negligible,and an increase of body weight was characteristic throughout the experimental period.The widespread loss of myenteric neurons,and marked but transient HO-1 up-regulation were demonstrated after the first TNBS administration.After repeated doses the length of the recovery time and extent of the ulcerous colonic segments were markedly decreased,and the neuronal loss was on a smaller scale and was limited to the inflamed area.HO-1 m RNA level was notably greater than after a single dose and overexpression was sustained throughout the timepoints examined.Nevertheless,the HO-1protein up-regulation after the second TNBS treatment proved to be transient.Following the third treatment HO-1 protein expression could not be detected.CONCLUSION:Experimentally provoked RRI may exert a protective preconditioning effect against the mucosal and neuronal damage.The persistent up-regulation of HO-1 m RNA expression may correlate with this. | Petra Talapka Lajos István Nagy Alexandra Pál Marietta Zita Poles Anikó Berkó Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2014 | World Journal of Gastroenterology2014，20，44： | 3 |
| 2 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 3 | Analysis of uncertain structural systems using interval analysis显示文摘 | Rao S S Berke L | 1997 | AIAA J1997，35，4： | 1 |
| 4 | Analysis of uncertain structural system using interval analysis显示文摘 | Rao S S Berke L | 1997 | AIAA Journal1997，35，4： | 1 |
| 5 | The Effects of Lidocaine and Adrenergic Agonists on Rat Sciatic Nerve and Skeletal Muscle Blood Flow In Vivo显示文摘 | Palmer G M Cairns B E Berkes S L | 2002 | Anesth Analg2002，95，： | 1 |
| 6 | Palliative radiotherapy for bone metastases: an ASTRO evidence-based guide- line显示文摘 | Lutz S Berk L Chang E | 2011 | Int J Radiat Oncol Biol Phys2011，79，4： | 1 |
| 7 | Analysis of uncertain structural systems using interval analysis 显示文摘 | Rao S S Berke L | 1997 | AIAA Journal1997，35，4： | 1 |
| 8 | Palliative radiotherapy for bone me- tastases :an ASTRO evidence-based guideline显示文摘 | Lutz S Berk L Chang E | 2011 | Int J Radiat Oncol Biol Phys2011，79，4： | 1 |
| 9 | Analysis of uncertain structural system using interval analysis显示文摘 | Rao S S Berke L | 1997 | A/AA Journal1997，35，4： | 1 |
| 10 | Analysis of uncertain structural systems u- sing interval analysis 显示文摘 | Rao S S Berke L | 1997 | AIAA Jounral1997，35，4： | 1 |
| 11 | Palliative radiotherapy for bone metastases:an ASTRO evidence-based guideline显示文摘 | Lutz S Berk L Chang E | | 0，，04： | 1 |
| 12 | Analysis of uncertain structural systems using interval analysis 显示文摘 | Rao S S Berk L | 1997 | AIAA Journal1997，35，4： | 1 |
| 13 | Palliative radiotherapy for bone metastases: an ASTRO evidence-based guideline显示文摘 | Lutz S Berk L Chang E | 2011 | Int J Radiat Oncol Biol Phys2011，79，4： | 1 |
| 14 | Palliative radiotherapy for bone me- tastases : an ASTRO evidence-based guideline 显示文摘 | Lutz S Berk L Chang E | 2011 | Int J Radiat Oneol Biol Phys2011，79，4： | 1 |
| 15 | Analysis of uncertain structural system using interval analysis 显示文摘 | RAO S S BERKE L | 1997 | AIAA Journal1997，35，4： | 1 |
| 16 | Combined effects of rosu- vastatin and pioglitazone in attenuating interferon gam- ma, tumor necrosis factor alpha, interleukin-6, C-reac- tive protein, and increasing intedeukin-4 may provide cardioprotection for patients with diabetes 显示文摘 | Tan L Berk L Tan S | 2006 | Chest Supplement2006，130，4： | 1 |
| 17 | What drives plan implementation? plans, planning agencies and developer 显示文摘 | LAURIEN L DAY M BACKHURST M BERKE P ERICKSEN N CRAWFORD J DIXON J CHAPMAN S | 2004 | Journal of Environmental Planning and Management2004，47，4： | 1 |
| 18 | Analysis of uncertain structural sys- tems using interval analysis显示文摘 | Rao S S Berke L | 1997 | AIAA Journal1997，35，4： | 1 |
| 19 | Analysis of uncertain structural system using interval analysis显示文摘 | Rao S S Berke L | 1997 | AIAA Journal1997，35，4： | 1 |
| 20 | Analysis of uncertain structural system using interval analysis显示文摘 | RAO S S BERKE L | 1997 | AIAA Journal1997，35，4： | 1 |
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