|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | The Synthesis of a New Dumbbell-shaped Compound of Bis-4,4'-bipyridine Bridged by 2,2'-Bipyridine显示文摘The title compound 5 was synthesized in 45% yield by the reaction of compound 3 with a, a-bis (bromomethyl)-2, 2-bipyridine in CH3CN at 70℃ for 24 h. | XinHuaXU RuiLiangLU BingZHOU JianPingLI YinQuanDENG | 2004 | Chinese Chemical Letters2004，15，2： | 4 |
| 2 | Transmitting-mode time reversal imaging using MUSIC algorithm for surveillance in wireless sensor network显示文摘 | LIU Xiaofei WANG Bingzhou | 2012 | IEEE Transactions on Antennas and Propagation2012，60，1： | 1 |
| 3 | A genetic algorithm used for the intensity level discretization in MLC leaf sequencing for step and shoot IMRT显示文摘The inverse planning for a step-and-shoot plan in intensity-modulated radiotherapy (IMRT) is usually a multiple step process. Before being converted into the MLC segments, the optimum intensity profiles of beams, which are generated by an optimization algorithm, shall be discretized into a few intensity levels. The discretization process of the optimum intensity profiles can induce deviations in the final dose distribution from the original optimum dose distribution. This paper describes a genetic algorithm for the discretization of given optimum intensity profiles. The algorithm minimizes an objective function written in terms of the intensity levels. Both the dose-based objective function, which is defined by the deviation between the dose distributions before and after the discretization, and the intensity-based objective function, which is defined by the deviation between the optimum intensity profiles and the discretization intensity profiles, have been adopted. To evaluate this algorithm, a series of simulation calculations had been carried out using the present algorithm, the even-spaced discretization and the k-means clustering algorithm respectively. By comparing the resultant discretization-induced deviations (DIDs) in intensity profiles and in dose distributions, we have found that the genetic algorithm induced less DIDs in comparison with that induced in the even-spaced discretization or the k-means clustering algorithm. Additionally, it has been found that the DIDs created in the genetic algorithm correlate with the complexity of the intensity profiles that is measured by the 'fluence map complexity'. | CHEN Bingzhou ZHANG Conghua TANG Zhiquan HOU Qing | 2008 | Nuclear Science and Techniques2008，19，1： | 1 |
| 4 | Assessing service protocol adaptability based on protocol reduction and graph search 显示文摘 | Zhang Bingzhou Bhiri S Hai Zhuge | 2011 | Concurrency and Computation: Practice and Ex- perience2011，23，9： | 1 |
| 5 | Microstructure and Fracture Morphology in the Welding Zone of T91 Heat-resisting Steel Used in Power Station显示文摘Microstructure performance in the welding zone of T91 heat-resistant steel under the condition of TIG welding wasresearched by means of metallography, X-ray diffraction and scanning electron microscope (SEM). Experimentalresults indicated that microstructure of T91 weld metal was austenite + a little amount of δ ferrite when using TGS-9cb filler wire. Substructure inside the austenite grain was crypto-crystal lath martensite, on which some Cr23C6blocky carbides were distributed. The maximum hardness (HRC44) in the welding zone is near the fusion zone. Thereexisted no obvious softening zone in the heat-affected zone (HAZ). For T91 steel tube of φ63 mmx5 mm, whenincreasing welding heat input (E) from 4.8 kJ/cm to 12.5 kJ/cm, fracture morphology in the fusion zone and theHAZ changed from dimple fracture into quasi-cleavage fracture (QC). Controlling the welding heat input of about9.8 kJ/cm is suitable in the welding of T91 heat-resistant steel. | YajiangLI BingZHOU TaoFENG JiangweiREN | 2002 | Journal of Materials Science & Technology2002，18，5： | 0 |
| 6 | Bi-FoRe:an efficient bidirectional knockin strategy to generate pairwise conditional alleles with fluorescent indicators显示文摘Gene expression labeling and conditional manipulation of gene function are important for elaborate dissection of gene function.However,contemporary generation of pairwise dual-function knockin alleles to achieve both conditional and geno-tagging effects with a single donor has not been reported.Here we first developed a strategy based on a flipping donor named FoRe to generate conditional knockout alleles coupled with fluorescent allele-labeling through NHEJ-mediated unidirectional targeted insertion in zebrafish facilitated by the CRISPR/Cas system.We demonstrated the feasibility of this strategy at sox10 and isl1 loci,and successfully achieved Cre-induced conditional knockout of target gene function and simultaneous switch of the fluorescent reporter,allowing generation of genetic mosaics for lineage tracing.We then improved the donor design enabling efficient one-step bidirectional knockin to generate paired positive and negative conditional alleles,both tagged with two different fluorescent reporters.By introducing Cre recombinase,these alleles could be used to achieve both conditional knockout and conditional gene restoration in parallel;furthermore,differential fluorescent labeling of the positive and negative alleles enables simple,early and efficient realtime discrimination of individual live embryos bearing different genotypes prior to the emergence of morphologically visible phenotypes.We named our improved donor as Bi-FoRe and demonstrated its feasibility at the sox10 locus.Furthermore,we eliminated the undesirable bacterial backbone in the donor using minicircle DNA technology.Our system could easily be expanded for other applications or to other organisms,and coupling fluorescent labeling of gene expression and conditional manipulation of gene function will provide unique opportunities to fully reveal the power of emerging single-cell sequencing technologies. | Bingzhou Han Yage Zhang Xuetong Bi Yang Zhou Christopher JKrueger Xinli Hu Zuoyan Zhu Xiangjun Tong Bo Zhang | 2021 | Protein & Cell2021，12，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员