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| 1 | Effect of Palrnatine on lipopolysaccharide-induced acute lung injury by inhibiting activation of the Akt/NF-κB pathway显示文摘Inflammation plays an important role in the development of acute lung injury(ALI).Severe pulmonary inflammation can cause acute respiratory distress syndrome(ARDS)or even death.Expression of proinflammatory interleukin-1β(IL-1β)and inducible nitric oxide synthase(iNOS)in the process of pulmonary inflammation will further exacerbate the severity of ALI.The purpose of this study was to explore the effect of Palrnatine(Pa)on lipopolysaccharide(LPS)-induced mouse ALI and its underlying mechanism.Pa,a natural product,has a wide range of pharmacological activities with the potential to protect against lung injury.Western blotting and quantitative real-time polymerase chain reaction(qRT-PCR)assays were performed to detect the expression and translation of inflammatory genes and proteins in vitro and in vivo.Immunoprecipitation was used to detect the degree of P65 translocation into the nucleus.We also used molecular modeling to further clarify the mechanism of action.The results showed that Pa pretreatment could significantly inhibit the expression and secretion of the inflammatory cytokine IL-1β,and significantly reduce the protein level of the proinflammatory protease iNOS,in both in vivo and in vitro models induced by LPS.Further mechanism studies showed that Pa could significantly inhibit the activation of the protein kinase B(Akt)/nuclear factor-κB(NF-κB)signaling pathway in the LPS-induced ALI mode and in LPS-induced RAW264.7 cells.Through molecular dynamics simulation,we observed that Pa was bound to the catalytic pocket of Akt and effectively inhibited the biological activity of Akt.These results indicated that Pa significantly relieves LPS-induced ALI by activating the Akt/NF-κB signaling pathway. | Xingchi KAN Yingsheng CHEN Bingxu HUANG Shoupeng FU Wenjin GUO Xin RAN Yu CAO Dianwen XU Ji CHENG Zhanqing YANG Yanling XU | 2021 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2021，22，11： | 1 |
| 2 | Down-Regulation of Stromal Caveolin-1 Expression in Esophageal Squamous Cell Carcinoma: A Potent Predictor of Lymph Node Metastases, Early Tumor Recurrence, and Poor Prognosis显示文摘 | Yibin Jia Nana Wang Jianbo Wang Hui Tian Wei Ma Kai Wang Bingxu Tan Guangyu Zhang Shengsi Yang Bing Bai Yufeng Cheng | 2014 | Annals of Surgical Oncology2014，，1： | 1 |
| 3 | Multi-omics analysis reveals the molecular regulatory network underlying the prevention of Lactiplantibacillus plantarum against LPS-induced salpingitis in laying hens显示文摘Background Salpingitis is one of the common diseases in laying hen production, which greatly decreases the economic outcome of laying hen farming. Lactiplantibacillus plantarum was effective in preventing local or systemic inflammation, however rare studies were reported on its prevention against salpingitis. This study aimed to investigate the preventive molecular regulatory network of microencapsulated Lactiplantibacillus plantarum(MLP) against salpingitis through multi-omics analysis, including microbiome, transcriptome and metabolome analyses.Results The results revealed that supplementation of MLP in diet significantly alleviated the inflammation and atrophy of uterus caused by lipopolysaccharide(LPS) in hens(P < 0.05). The concentrations of plasma IL-2 and IL-10 in hens of MLP-LPS group were higher than those in hens of LPS-stimulation group(CN-LPS group)(P < 0.05). The expression levels of TLR2, MYD88, NF-κB, COX2, and TNF-α were significantly decreased in the hens fed diet supplemented with MLP and suffered with LPS stimulation(MLP-LPS group) compared with those in the hens of CN-LPS group(P < 0.05). Differentially expressed genes(DEGs) induced by MLP were involved in inflammation, reproduction, and calcium ion transport. At the genus level, the MLP supplementation significantly increased the abundance of Phascolarctobacterium, whereas decreased the abundance of Candidatus_Saccharimonas in LPS challenged hens(P < 0.05). The metabolites altered by dietary supplementation with MLP were mainly involved in galactose, uronic acid, histidine, pyruvate and primary bile acid metabolism. Dietary supplementation with MLP inversely regulates LPSinduced differential metabolites such as Lyso PA(24:0/0:0)(P < 0.05).Conclusions In summary, dietary supplementation with microencapsulated Lactiplantibacillus plantarum prevented salpingitis by modulating the abundances of Candidatus_Saccharimonas, Phascolarctobacterium, Ruminococcus_torques_group and Eubacterium_hallii_group while downregulating the levels of plasma metabolites, p-tolyl sulfate, o-cresol and N-acetylhistamine and upregulating S-lactoylglutathione, simultaneously increasing the expressions of CPNE4, CNTN3 and ACAN genes in the uterus, and ultimately inhibiting oviducal inflammation. | Dan Song Aike Li Bingxu Chen Jia Feng Tao Duan Junlin Cheng Lixian Chen Weiwei Wang Yuna Min | 2024 | Journal of Animal Science and Biotechnology2024，15，1： | 0 |
| 4 | Acupuncture and moxibustion for malignant tumor patients with psychological symptoms of insomnia,anxiety and depression:a systematic review and Meta-analysis显示文摘OBJECTIVE:To evaluate the efficacy and safety of acupuncture and moxibustion therapy(AMT) for cancerrelated psychological symptoms(CRPS) of insomnia,depression and anxiety.METHODS:Seven databases were searched for randomized controlled trials(RCT) comparing AMT to routine care or conventional drug for alleviating CRPS of insomnia,depression,and anxiety before April 2020.Two independent reviewers performed the data extraction and assessed the risk of bias.RESULTS:A total of 30 RCTs involving 2483 cancer patients were enrolled.The pooled analysis indicated that the treatment group was significantly better than the control group in improving the depression effective rate [RR = 1.29,95% CI(1.12,1.49),P = 0.0004],the quality of life(QOL) [MD = 1.11,95% CI(0.80,1.42),P < 0.000 01],and reducing Self-rating Anxiety Scale(SAS) [MD =﹣7.75,95% CI(﹣10.44,﹣5.05),P < 0.000 01].But there was no statistically significant difference between two groups in improving the insomnia effective rate [RR = 1.18,95% CI(0.93,1.51),P = 0.18].The subgroup analysis showed the effectiveness of different intervention on CRPS.Compared with routine care,AMT helps relieve CRPS better evaluated by Pittsburgh Sleep Quality Index(PSQI),Hamilton Depression Scale(HAMD),and Self-rating Depression Scale(SDS),and depression effective rate.Compared with conventional drug,AMT performs better evaluated by SDS,depression effective rate and QOL.Moreover,the conventional drug showed higher treatment efficacy on improving insomnia effective rate compared with AMT.Compared to conventional drug,AMT plus conventional drug resulted in a significant reduction on CRPS such as PSQI,HAMD,SDS,and SAS,and also had a meaningful improvement on insomnia effective rate,depression effective rate and QOL.Fewer published reports were found on the adverse events of AMT than the conventional drug.CONCLUSION:The results suggested that AMT might be effective in improving CPRI;however,a definite conclusion could not be drawn because the quality of trials are low.Further large-scale and high-quality RCTs to verify the efficacy and safety of AMT on CRPS are still warranted. | MA Fangfang ZHANG Hewei LI Bingxue CHENG Peiyu YU Mingwei WANG Xiaomin | 2023 | Journal of Traditional Chinese Medicine2023，43，3： | 0 |
| 5 | Motor oil degradation during urban cycle road tests显示文摘Several civilian vehicles in China operate in urban traffic conditions and have their motor oil changed every 5,000 km.This study investigates the variations in oil properties after servicing at 5,000 km,based on systematic road tests(including a repeated test,a parallel test,and a new vehicle test).The physicochemical properties,changes in components,oxidation stability,detergent‐dispersant performance,and tribological properties of motor oils were analyzed.The results showed that the total acid number(TAN)of oils increased with the operation mileage,by up to 1.41 mgKOH/g.The total base number(TBN)decreased after the road tests were completed,and the decrease was less than 44.6%.The kinematic viscosity(KV)of most oils decreased initially and then stabilized in the middle stage,before starting to increase later in the experiment.The change in KV at 100℃was less than 15.96%.The oxidation onset temperature(OOT)of the oils diminished gradually with the operation mileage.All OOT values of the used oils were higher than 210℃.A spot test indicated that the used oils retained their detergentdispersant performance to an appropriate extent.The four‐ball wear scar diameters and friction coefficient of the used oils did not increase significantly after the road tests were completed.This study can serve as a reference for end‐users when changing motor oils. | Lei WEI Haitao DUAN Yongliang JIN Dan JIA Bingxue CHENG Jianfang LIU Jian LI | 2021 | Friction2021，9，5： | 0 |
| 6 | Method for the measurement of triboelectric charge transfer at solid–liquid interface显示文摘Triboelectrification between a liquid and a solid is a common phenomenon in our daily life and industry.Triboelectric charges generated at liquid/solid interfaces have effects on energy harvesting,triboelectrification-based sensing,interfacial corrosion,wear,lubrication,etc.Knowing the amount of triboelectric charge transfer is very useful for studying the mechanism and controlling these phenomena,in which an accurate method is absolutely necessary to measure the triboelectric charge generated at the solid–liquid interface.Herein,we established a method for measuring the charge transfer between different solids and liquids.An equipment based on the Faraday cup measurement was developed,and the leakage ratio(r_(l))was quantified through simulation based on an electrostatic field model.Typical experiments were conducted to validate the reliability of the method.This work provides an effective method for charge measurement in triboelectrification research. | Qin CHEN Bingxue CHENG Tiancheng WANG Hongfei SHANG Tianmin SHAO | 2023 | Friction2023，11，8： | 0 |
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