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| 1 | 基于MDCK悬浮细胞灌注培养的流感病毒生产过程开发显示文摘为了提升用MDCK悬浮细胞培养生产流感疫苗的效率,克服目前存在的产能低下问题,以期满足日益增长的流感疫苗市场需求和提升应对大流行爆发的能力。通过病毒驯化、稀释流加条件下的细胞生长和产毒能力分析、Semi-perfusion中的细胞高密度培养可行性分析以及ATF灌注反应器过程验证完成过程开发。驯化的病毒加速了其在细胞内的感染扩增,且在稀释流加培养中获得(3.57±0.17)log10(HAU/100μL)的病毒滴度。Semi-perfusion可实现细胞高密度培养至40×106 cells/mL以上,病毒产量达4 log10(HAU/100μL)以上,同时发现MOI和胰酶浓度对病毒的复制过程存在显著影响。在反应器中的ATF灌注培养过程得到了与Semi-perfusion相近的细胞密度及更高的病毒产量4.37 log10(HAU/100μL),并且通过降温措施维持了高密度下的单细胞病毒产量,克服了“细胞密度效应”。建立了基于MDCK悬浮细胞ATF灌注培养的流感病毒生产平台,通过同时提高细胞密度和单细胞病毒产率提升了流感病毒的生产效率,为基于细胞培养的流感疫苗产业化生产提供了新选择。 | 吴熠潇 Thomas Bissinger Yvonne Genzel 刘旭平 Udo Reichl 谭文松 | 2020 | 生物技术通报2020，36，10： | 2 |
| 2 | Postoperative residual paralysis and respiratory ststus:a comparative study of pancuronium and vecuronium显示文摘 | Bissinger U Schimek F Lenz G | 2000 | Physiol Res2000，49，4： | 1 |
| 3 | The effect of! diabetic autonomic neuropathy on P--wave duration,dispersiot / and atrial fibrillation显示文摘 | Bissinger A Grycewicz T Grabowicz W | 2011 | Arch Med Sci2011，7，5： | 1 |
| 4 | Synthesis and biological testing of novel pyrido- isothiazolones as histone acetyltransferase inhibitors 显示文摘 | FURDAS S D SHEKFEH S BISSINGER E M | 2011 | Bioorg Med Chem2011，19，12： | 1 |
| 5 | Endothelial function and left ventricular remodeling in diabetic and non-diabetic patients after acute coronary syndrome显示文摘 | Bissinger A Grycewicz T Grabowicz W | | 0，，： | 1 |
| 6 | Thermoregulation in very low birth-weight infants during the golden hour:results and implications显示文摘 | Bissinger RL Annibale DJ | 2010 | Adv Neonatal Care2010，10，： | 1 |
| 7 | Human cytomegalovirus as a direct pathogen:correlation of multiorgan involvement and cell distribution with clinical and pathological findings in a case of congential inclusion disease显示文摘 | Bissinger AL Sinzger C Kaiserling E | 2002 | J Med Virol2002，67，2： | 1 |
| 8 | Recurrence of ventricular arrhythmias in patients with non-ischaemic dilated cardiomyopathy:evidence-based predictors显示文摘 | Dulak E Lubifiski A Bissinger A | | 0，，08： | 1 |
| 9 | Determinants of geographic variations in implantation of cardiac defibrillators in the European Society of Cardiology member countries-data from the European Heart Rhythm Association White Book显示文摘 | Lubinski A Bissinger A Boersma L | 2011 | Europace2011，13，5： | 1 |
| 10 | Tryptophan mutants in Arabidopsis: the consequences of duplicated tryptophan synthase β genes显示文摘 | Last R L Bissinger P H Mahoney D J | 1991 | Plant Cell1991，3，： | 1 |
| 11 | Downreg- ulation of peptide transporters PEPT1 and PEPT2 by oxidative stress responsive kinase OSR1显示文摘 | WARSI J ELVIRA B BISSINGER R | 2014 | Kidney & Blood Pressure Research2014，39，6： | 1 |
| 12 | Fluoxetine induced suicidal erythrocyte death显示文摘 | Jilani K Enkel S Bissinger R | 2013 | Toxins2013，5，7： | 1 |
| 13 | Global modeling of the violin radiativity pro- file显示文摘 | Bissinger G | 2010 | The Journal of the Aeoustieal Society of Ameri- ca2010，127，3： | 1 |
| 14 | Real-time intrusion detection in power system operations显示文摘 | Valenzuela J Wang J Bissinger N | 2013 | IEEE Transactions on Power Systems2013，28，2： | 1 |
| 15 | Passive Sonar Tracking Using Sequences of Received Signal Amplitude Fluctuations:Dependence on Environmental Sampling显示文摘 | R Lee Culver Brett E Bissinger Alex W Sell | | 0，，04： | 1 |
| 16 | Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae 显示文摘 | Davidson J F Whyte B Bissinger P H | 1996 | Proc Natl Acad Sci USA1996，93，： | 1 |
| 17 | Value and circadian variations of QT dispersion in patients with dia- betes mellitus and coronary artery disease显示文摘 | BISSINGER A MARKUSZEWSKI L ROSIAK M | 2008 | Pal Merkur Le- kaki2008，24，140： | 1 |
| 18 | Real-time intrusion detection in power system operations显示文摘 | Valenzuela J Wang J H Bissinger N | 2013 | IEEE Transactions on Power Systems2013，28，2： | 1 |
| 19 | Comparative efficacy of BioUD to other commercially available arthropod repellents against the ticks, Amblyomma americanum and Dermacentor variabilis on cotton cloth显示文摘 | Bissinger BW Zhu J Apperson CS | 2009 | Am J Trop Med Hyg2009，81，4： | 1 |
| 20 | Postoperative residual paralysis and respiratory status:a comparative study of pancurenium and vecurenium显示文摘 | Bissinger U Schimek F Lenz G | 2000 | Physiol Res2000，49，4： | 1 |
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