| 1 | Amygdalin attenuates PM2.5-induced human umbilical vein endothelial cell injury via the TLR4/NF-κB and Bcl-2/Bax signaling pathways显示文摘Mounting evidence supports that long-term exposure to fine particle pollutants(PM2.5)is closely implicated in cardiovascular diseases,especially atherosclerosis.Amygdalin is reported to attenuate external stimuli-induced cardiovascular diseases.However,the underlying mechanisms are still not understood.In this study,we aim to explore the protective effects of amygdalin on PM2.5-induced human umbilical vein endothelial cell(HUVEC)injury and unravel the specific mechanisms by MTT,DCFH-DA,biochemical,immunofluorescence,ELISA,RT-qPCR,flow cytometry,TUNEL and western blot analysis.The results reveal that amygdalin reverses PM2.5-induced cytotoxicity and attenuates intracellular ROS production.Moreover,amygdalin increases the levels of SOD and GSH and alleviates the MDA content.Additionally,amygdalin causes a decline of IL-6,IL-1β,TNF-αand COX-2 levels.Moreover,amygdalin inhibits NF-κB p50 and TLR4 protein expressions and NF-κB p65 nuclear translocation.Concomitantly,a decline of phospho-NF-κB p65/NF-κB p65 and phospho-IκB-α/IκB-αis detected.Meanwhile,amygdalin pretreatment reduces HUVEC apoptosis.In addition,amygdalin triggers an upregulation of Bcl-2 and a downregulation of Bax after stimulation with PM2.5.Collectively,these results suggest that amygdalin suppresses PM2.5-induced HUVEC injury by regulating the TLR4/NF-κB and Bcl-2/Bax signaling pathways,indicating that amygdalin may be a novel target for atherosclerosis treatments. | Bixu Wang Tong Sun Ling Sun Lan Li Haitong Wan Zhishan Ding Xiaoqing Ye | 2022 | Acta Biochimica et Biophysica Sinica2022，54，10： | 1 |
| 2 | Decellularized extracellular matrix and mesenchymal stem cells promote recovery in traumatic brain injury by synergistically enhancing neurogenesis and attenuating neuroinflammation显示文摘Damaged neurons and harsh microenvironments contribute to the injury cascades following traumatic brain injury(TBI).Mesenchymal stem cell(MSC)therapy is considered a viable choice for brain in-jury treatment;however,its clinical use is hindered by limited engraftment,low survival ratio,and uncontrolled differentiation.Herein,the decellularized brain extracellular matrix(dBECM)was prepared through a new method as a delivery system for MSCs.dBECM-based hydrogel with favorable biochem-ical and biomechanical features provides a microenvironment for MSCs in nerve repair.The MSCs-encapsulated dBECM-based hydrogel is shown to improve neuron compensation and structural regen-eration,alleviate neuroinflammation,and promote M1-to-M2 polarization of microglia.Our results first demonstrate that the combination of dBECM and MSCs is a critical vehicle to promote TBI repair from neurogenesis and immunoregulation,showing promise for the physiological recovery of neurogenic dis-eases and injuries. | Xuewei Zhang Bixue Wang Hua Hong Ying Wang Jiashang Liu Changsheng Liu Xi Chen | 2023 | Journal of Materials Science & Technology2023，，7： | 0 |
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