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| 1 | Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level显示文摘Mesenchymal stem cells(MSCs)have been cited as contributors to heart repair through cardiogenic differentiation and multiple cellular interactions,including the paracrine effect,cell fusion,and mechanical and electrical couplings.Due to heart–muscle complexity,progress in the development of knowledge concerning the role of MSCs in cardiac repair is heavily based on MSC–cardiomyocyte coculture.In conventional coculture systems,however,the in vivo cardiac muscle structure,in which rod-shaped cells are connected end-to-end,is not sustained;instead,irregularly shaped cells spread randomly,resulting in randomly distributed cell junctions.Consequently,contact-mediated cell–cell interactions(e.g.,the electrical triggering signal and the mechanical contraction wave that propagate through MSC–cardiomyocyte junctions)occur randomly.Thus,the data generated on the beneficial effects of MSCs may be irrelevant to in vivo biological processes.In this study,we explored whether cardiomyocyte alignment,the most important phenotype,is relevant to stem cell cardiogenic differentiation.Here,we report(i)the construction of a laser-patterned,biochip-based,stem cell–cardiomyocyte coculture model with controlled cell alignment;and(ii)single-cell-level data on stem cell cardiogenic differentiation under in vivo-like cardiomyocyte alignment conditions. | Zhen Ma Qiuying Liu Huaxiao Yang Raymond B Runyan Carol A Eisenberg Meifeng Xu Thomas K Borg Roger Markwald Yifei Wang Bruce Z Gao | 2013 | Light(Science & Applications)2013，2，1： | 2 |
| 2 | Suagrcane yellow leaf virus : a novel member of the Luteoviridae that probably arose by inter-species recombination 显示文摘 | Smith G R Borg Z Lockhart B E | 2000 | J Gen Virol2000，81，： | 1 |
| 3 | Simultaneous follow-up of mouse colon lesions by colonoscopy and endoluminal ultrasound biomicroscopy显示文摘AIM:To evaluate the potential use of colonoscopy and endoluminal ultrasonic biomicroscopy(eUBM)to track the progression of mouse colonic lesions.METHODS:Ten mice were treated with a single azoxy-methane intraperitoneal injection(week 1)followed by seven days of a dextran sulfate sodium treatment in their drinking water(week 2)to induce inflammationassociated colon tumors.eUBM was performed simultaneously with colonoscopy at weeks 13,17-20 and21.A 3.6-F diameter 40 MHz mini-probe catheter was used for eUBM imaging.The ultrasound mini-probe catheter was inserted into the accessory channel of a pediatric flexible bronchofiberscope,allowing simultaneous acquisition of colonoscopic and eUBM images.During image acquisition,the mice were anesthetized with isoflurane and kept in a supine position over a stainless steel heated surgical waterbed at 37℃.Both eUBM and colonoscopic images were captured and stored when a lesion was detected by colonoscopy or when the eUBM image revealed a modified colon wall anatomy.During the procedure,the colon was irrigated with water that was injected through a flush port on the mini-probe catheter and that acted as the ultrasound coupling medium between the transducer and the colon wall.Once the acquisition of the last eUBM/colonoscopy section for each animal was completed,the colons were fixed,paraffin-embedded,and stained with hematoxylin and eosin.Colon images acquired at the first time-point for each mouse were compared with subsequent eUBM/colonoscopic images of the same sites obtained in the following acquisitions to evaluate lesion progression.RESULTS:All 10 mice had eUBM and colonoscopic images acquired at week 13(the first time-point).Two animals died immediately after the first imaging acquisition and,consequently,only 8 mice were subjected to the second eUBM/colonoscopy imaging acquisition(at the second time-point).Due to the advanced stage of colonic tumorigenesis,5 animals died after the second time-point image acquisition,and thus,only three were subjected to the third eUBM/colonoscopy imaging acquisition(the third time-point).eUBM was able to detect the four layers in healthy segments of colon:the mucosa(the first hyperechoic layer moving away from the mini-probe axis),followed by the muscularis mucosae(hypoechoic),the submucosa(the second hyperechoic layer)and the muscularis externa(the second hypoechoic layer).Hypoechoic regions between the mucosa and the muscularis externa layers represented lymphoid infiltrates,as confirmed by the corresponding histological images.Pedunculated tumors were represented by hyperechoic masses in the mucosa layer.Among the lesions that decreased in size between the first and third time-points,one of the lesions changed from a mucosal hyperplasia with ulceration at the top to a mucosal hyperplasia with lymphoid infiltrate and,finally,to small signs of mucosal hyperplasia and lymphoid infiltrate.In this case,while lesion regression and modification were observable in the eUBM images,colonoscopy was only able to detect the lesion at the first and second time-points,without the capacity to demonstrate the presence of lymphoid infiltrate.Regarding the lesions that increased in size,one of them started as a small elevation in the mucosa layer and progressed to a pedunculated tumor.In this case,while eUBM imaging revealed the lesion at the first time-point,colonoscopy was only able to detect it at the second time-point.All colonic lesions(tumors,lymphoid infiltrate and mucosal thickening)were identified by eUBM,while colonoscopy identified just76%of them.Colonoscopy identified all of the colonic tumors but failed to diagnose lymphoid infiltrates and increased mucosal thickness and failed to differentiate lymphoid infiltrates from small adenomas.During the observation period,most of the lesions(approximately67%)increased in size,approximately 14%remained unchanged,and 19%regressed.CONCLUSION:Combining eUBM with colonoscopy improves the diagnosis and the follow-up of mouse colonic lesions,adding transmural assessment of the bowel wall. | Rossana C Soletti Kelly Z Alves Marcelo AP de Britto Dyanna G de Matos Mnica Soldan Helena L Borges Joo C Machado | 2013 | World Journal of Gastroenterology2013，19，44： | 1 |
| 4 | Quantitative effect of CYP2D6 genotype and inhibitors on tamoxifen metabolism:implication for optimization of breast cancer treatment显示文摘 | Borges S Desta Z Li L | 2006 | Clin Pharmacol Ther2006，80，1： | 1 |
| 5 | Quantitative effect of CYP2D6 genotype and inhibitors on tamoxifen metabolism : implication for optimization of breast cancer treatment显示文摘 | Borges S Desta Z Li L | 2006 | Clin Pharmacol Ther2006，80，： | 1 |
| 6 | Pigment epithelium-derived factor regulates lipid metabolism via adipose triglyceride lipase显示文摘 | Borg M L Andrews Z B Duh E J | 2011 | Dia- betes2011，60，5： | 1 |
| 7 | Fusion of dendritic cells with multiple myeloma cells results in maturation and enhanced antigen presentation显示文摘 | Vasir B Borges V Wu Z | 2005 | Br J Haematol2005，129，5： | 1 |
| 8 | Sugarcane yellow leaf virus: a novel member of the Luteoviridae that probably arose by interspecies recombination 显示文摘 | Smith G R Borg Z Lockhart B E L | 2000 | Journal of General Virology2000，81，： | 1 |
| 9 | Quantitative effect of CYP2D6 genotype and inhibitors on tamoxifen metabolism:implication for optimization of breast cancer treatment显示文摘 | Borges S Desta Z Li L | 2006 | Clin Pharmacol Ther2006，80，1： | 1 |
| 10 | Sugarcane yellow leaf virus:a novel member of the Luteoviridae that probably a-rose by inter-species recombination显示文摘 | Smith G R Borg Z Lockhart B E L | | 0，，07： | 1 |
| 11 | Immunization with hepatitis C virus-like particles results in control of hepatitis C virus infection in chimpanzees 显示文摘 | Elmowalid GA Qiao M Jeong SH Borg BB Baumert TF Sapp RK Hu Z Murthy K Liang TJ | 2007 | Proc Natl Acad Sci USA2007，104，20： | 1 |
| 12 | Sugarcane yellow leaf virus:a novel member of the Luteoviridae that probably arose by interspecies recombination显示文摘 | SMITH G R BORG Z LOCKHART B E L | 2000 | Journal of General Virology2000，81，： | 1 |
| 13 | Quantitative effect of CYP2D6 genotype and inhibitorson tamoxifen metabolism:Implication for optimization of breast cancer treatment显示文摘 | Borges S Desta Z Li Lang | 2006 | ClinPharmacol Ther2006，80，1： | 1 |
| 14 | Thermo-and light-responsivepolymers containing photoswitchable azobenzene end groups显示文摘 | Jochum F D Borg L Z Roth P J | 2009 | Macromolecules2009，42，20： | 1 |
| 15 | Yellow leaf of sugarcane is caused by at least three different genotypes of sugarcane yellow leaf virus,one of which predominates on the Island of Réunion显示文摘 | Abu Ahnad Y Rassaby L Royer M Borg Z Braithwaite K S Mirkov T E Irey M S Perrier X Smith G R and Rott P | | 0，，： | 1 |
| 16 | Spinal radiation before surgical docompression adversely affects outcomes of surgery for symptomatic metastatic spinal cord compression显示文摘 | Ghogawala Z Mansfield FL Borges LF | | 0，，： | 1 |
| 17 | Giant internal carotid artery aneurysm simulating pituitary adenoma显示文摘 | Borges F Z Ferreira B P Resende E A | 2006 | Arq Bras Endocrinol Metabol2006，50，3： | 1 |
| 18 | Quantitative effect of CYP2D6 geno- type and inhibitors on tamoxifen metabolism:implication for optimi- zation of breast cancer treatment显示文摘 | Borges S Desta Z Li L | 2006 | Clin Pharmacol Ther2006，80，1： | 1 |
| 19 | Sugarcane yellow leaf virus:a novel member of the Luteoviridae that probably arose by inter-species recomhination显示文摘 | Smith G R Borg Z Lockhart B E L Brnithwaite K S and Gibbs M | | 0，，： | 1 |
| 20 | Fischer-Tropsch synthesis: Cobalt particle size and particle size and support effects on intrinsic activity and product distribution显示文摘 | BORG ? DIETZEL P D C SPJELKAVIK A I TVETEN E Z WALMSLEY J C DIPLAS S ERI S HOLMEN A RYTTER E | 2008 | J Catal2008，259，： | 1 |
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