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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | 显示文摘 | Cvetko B F Brungs M P Burford R P | 1998 | Mater Sci1998，23，： | 2 |
| 3 | Musie therapy for reducing surgical显示文摘 | Brunges M Avigne G | 2003 | ARON J2003，78，5： | 1 |
| 4 | Music therapy for reducing surgical anxiety显示文摘 | Brunges M Avigne G | 2003 | ARONJ2003，78，5： | 1 |
| 5 | Music therapy for reducing surgical anxiety显示文摘 | Brunges MJ Avigne G | 2003 | AORN J2003，78，5： | 1 |
| 6 | Sol-gel processing by aging and pore creator addition for porous silica antiflective coatings显示文摘 | Wongcharee K Brungs M Chaplin R | 2002 | Journal of Sol-gel Science and Technology2002，25，： | 1 |
| 7 | New catalyst for the conversion of methane to synthesis gas:molybdenum and tungsten carbide 显示文摘 | Krylow O V York A P E Brungs A T | 1998 | Catal1998，180，1： | 1 |
| 8 | Intermittent androgen deprivation is a rational standard of care treatment for all stages of progressive prostate cancer: results from a systematic review and meta-analysis显示文摘 | BRUNGS D CHEN J MASSON P | 2014 | Prostate Cancer Prostatic Dis2014，17，2： | 1 |
| 9 | Gauss extensions and total graded subrings for crossed product algebras显示文摘 | BRUNGS H H MARUBAYASHI H OSMANAGIC E | 2007 | J Algebra2007，316，1： | 1 |
| 10 | Molybdenumand tungsten carbides as catalysts for the conversionof methane to synthesis gas using stoichiometricfeedstocks显示文摘 | York A P E Claridge J B Brungs A J | 1997 | Chemical Communications1997，100，1： | 1 |
| 11 | New catalyst for the conversion of methane to synthesis gas: molybdenum and tungsten carbide显示文摘 | Krylov O V York A P E Brungs A T | 1998 | J Catal1998，180，1： | 1 |
| 12 | Particle residence time distribution (RTD) in three-phase annular bubble column reactor 显示文摘 | Pareek V K Yap Z Brungs M P Adesina A A | 2001 | Chem Eng Sci2001，56，： | 1 |
| 13 | New catalysts for the conversion of methane to synthesis gas: Molybdenum and tungsten carbide显示文摘 | CLARIDGE J B YORK A P E BRUNGS A J MARQUEZ-ALVAREZ C SLOAN J TSANG S C GREEN M L H | 1998 | J Catal1998，180，1： | 1 |
| 14 | 显示文摘 | Cvetko B F Brungs M P Burford R P | 1998 | Mater Sci1998，23，： | 1 |
| 15 | Light Weight Design with Light Metal Castings 显示文摘 | BRUNGS D | 1997 | Materials and Design1997，18，456： | 1 |
| 16 | New catalysts for the conversion of methane to synthesis gas:Molybdenum and tungsten carbide显示文摘 | Claridge J B York A P E Brungs A J | | 0，，01： | 1 |
| 17 | Dry reforming of methane to synthesis gas over supported molybdenum carbide catalysts显示文摘 | BRUNGS A J YORK A P E CLARIDGE J B | 2000 | Catal Lett2000，70，34： | 1 |
| 18 | Music therapy for reducing surgical anxiety显示文摘 | Michele Brunges Gail Avigne | 2003 | AORN Journal2003，，5： | 1 |
| 19 | Degradation of sucrose and nitrate over titania coated nano -hematite hotocatalysts显示文摘 | Metta Penpolcharoen Rose Amall Michael Brungs | 2001 | Journal of Nanoparticle Research2001，，3： | 1 |
| 20 | New catalysts for the conversion of methane to synthesis gas: molybdenum and tungsten carbide 显示文摘 | Claridge J B York A P E Brungs A J | 1998 | J Catal1998，180，1： | 1 |
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