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| 1 | Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation显示文摘AIM:To provide further insight into the characterization of mucosa-associated Escherichia coli(E.coli)isolated from the colonic mucosa of cancer patients.METHODS:Phylogroups and the presence of cyclomodulin-encoding genes of mucosa-associated E.coli from colon cancer and diverticulosis specimens weredetermined by PCR.Adhesion and invasion experiments were performed with I-407 intestinal epithelial cells using gentamicin protection assay.Carcinoembryonic antigen-related cell adhesion molecule 6(CEACAM6)expression in T84 intestinal epithelial cells was measured by enzyme-linked immunosorbent assay and by Western Blot.Gut colonization,inflammation and procarcinogenic potential were assessed in a chronic infection model using CEABAC10 transgenic mice.Cell proliferation was analyzed by real-time mRNA quantification of PCNA and immunohistochemistry staining of Ki67.RESULTS:Analysis of mucosa-associated E.coli from colon cancer and diverticulosis specimens showed that whatever the origin of the E.coli strains,86%of cyclomodulin-positive E.coli belonged to B2 phylogroup and most harbored polyketide synthase(pks)island,which encodes colibactin,and/or cytotoxic necrotizing factor(cnf)genes.In vitro assays using I-407 intestinal epithelial cells revealed that mucosa-associated B2 E.coli strains were poorly adherent and invasive.However,mucosa-associated B2 E.coli similarly to Crohn’s disease-associated E.coli are able to induce CEACAM6expression in T84 intestinal epithelial cells.In addition,in vivo experiments using a chronic infection model of CEACAM6 expressing mice showed that B2 E.coli strain11G5 isolated from colon cancer is able to highly persist in the gut,and to induce colon inflammation,epithelial damages and cell proliferation.CONCLUSION:In conclusion,these data bring new insights into the ability of E.coli isolated from patients with colon cancer to establish persistent colonization,exacerbate inflammation and trigger carcinogenesis. | Jennifer Raisch Emmanuel Buc Mathilde Bonnet Pierre Sauvanet Emilie Vazeille Amélie de Vallée Pierre Déchelotte Claude Darcha Denis Pezet Richard Bonnet Marie-Agnès Bringer Arlette Darfeuille-Michaud | 2014 | World Journal of Gastroenterology2014，20，21： | 12 |
| 2 | Characterization of Ni O thin films deposited by reactive sputtering显示文摘 | I Hatov D Buc S Hascik | 1998 | Vacuum1998，50，12： | 1 |
| 3 | KRAS mutations as an independent prognostic factor in pa- tients with advanced colorectal cancer treated with cetuximab 显示文摘 | Lievre A Bachet J B Boige V Cayre A Le Corre D Buc E | 2008 | J Clin Oncol2008，26，： | 1 |
| 4 | Thermal Stablity of NbN Films Deposited on GaAs Substrates显示文摘 | Hotovy I Huran J Buc D el al | 1998 | Vacuum1998，50，12： | 1 |
| 5 | Cerebral infarct and the immune response显示文摘 | Bartko D Lesicky O Buc M | 1997 | Bratisl Lek Listy1997，98，6： | 1 |
| 6 | Phosphofiuctokinases from Escherichia coli显示文摘 | Kotlarz D Buc H | 1982 | Methods Encymol1982，90，： | 1 |
| 7 | Changes in visual evoked potentials curve parameters in children w ith amblyopia显示文摘 | Karlica D Galetovic'D Buc'an K Znaor L Skelin S | 2009 | Acta Med Croatica2009，63，4： | 1 |
| 8 | Massive hepatic necrosis with gastric, splenic, and pancreatic infarctions after ethanol ablation for hepatocellular carcinoma显示文摘 | Da Ines D Buc E Petitcolin V | 2010 | J Vase Interv Radiol2010，21，8： | 1 |
| 9 | Phosphofructokinases from Escherichia coli显示文摘 | Kotlarz D Buc H | 1982 | Methods Enzymol1982，90，： | 1 |
| 10 | Characterization of NiO thin films deposited by reactive sputtering显示文摘 | Hotovy I Buc D Hascik S | 1998 | Vacuum1998，50，1： | 1 |
| 11 | Cerebral infarct and the immune response显示文摘 | Bartko D Lesicky O Buc M | 1997 | Bratisl Lek Listy1997，98，6： | 1 |
| 12 | Cerebral infarct and the immune response显示文摘 | Bartko D Lesicky O Buc M | 1997 | Bratisl Lek Listy1997，98，6： | 1 |
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