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| 1 | 前列腺癌骨髓微转移的免疫细胞化学检测显示文摘目的:探讨前列腺癌患者骨髓中微转移癌细胞的检测方法。方法:术前采集前列腺癌59例的骨髓标本并收集相关临床资料,应用细胞角蛋白的免疫细胞化学方法进行检测,并将结果与TNM G分期(级)进行相关分析。结果:骨髓中CK+细胞检出率为25.4%,除G3/4组阳性率(45.5%)明显高于G1/2组(12.5%,P=0.01)外,其余各组间的阳性率,差异均无显著性意义。结论:免疫细胞化学方法可作为检测骨髓中隐蔽的微转移肿瘤细胞的一种敏感的标准方法,肿瘤细胞的分化程度可能与其离开原发灶发生播散的能力有关,该指标是一个独立的危险因素,对预后评估和治疗方法的选择与确定有指导意义。 | 黄瑜峰 Oberneder R Riesenberg R Buchner A Hofstetter A | 2001 | 临床泌尿外科杂志2001，16，5： | 2 |
| 2 | Renaturation, purification and characterization of recombinant Fab-fragments produced in Es- cherichia coli显示文摘 | BUCHNER J RUDOLPH R | 1991 | Bio-Technology (New York)1991，9，2： | 1 |
| 3 | International conference for the develop ment of a consensus on the management and prevention of severe candidal infections显示文摘 | Edwards J E Jr Bodey G P Bowden R A Buchner T de Pauw BE Filler SG | 1997 | Clin Infect Dis1997，25，： | 1 |
| 4 | A generalization of blind source separation algorithms for convolutive mixtures based on second-order statistics显示文摘 | Buchner H Aichner R Kellermann W | | 0，，01： | 1 |
| 5 | Carbon nanostructures as multi-functional drug delivery Platforms显示文摘 | Mendes R G Bachmatiuk A Buchner B Cuniberti G Rummeli M H | 2013 | Journal of Materials Chemistry B2013，，1： | 1 |
| 6 | A generalization of blind source separation algorithms for convolutive mixtures based on second order statistics显示文摘 | H Buchner R Aichner W Kellermann | 2005 | IEEE Trans on Speech and Audio Processing2005，13，1： | 1 |
| 7 | Hsp90 chaperones protein folding in vitro显示文摘 | Wiech H Buchner J Zimmermann R | 1992 | Nature1992，358，6382： | 1 |
| 8 | Temporal pattern of source activities evoked by different types of motion onset stimuli显示文摘 | Delon-Martin C Gobbele R Buchner H | | 0，，04： | 1 |
| 9 | Contact allergy as a therapeutic tool for alopecia areata: application of aquaric acid dibutylester显示文摘 | Happle R Kalveram KJ Buchner U | 1980 | Dermatologica1980，161，： | 1 |
| 10 | 根治术前后肿瘤细胞在骨髓中播散的自动显微图像研究显示文摘目的:对比研究泌尿系肿瘤患者根治术前后骨髓中散在的微转移肿瘤细胞。方法:术前及术后采集肾癌、前列腺癌和尿道膀胱癌共102例患者的骨髓标本,应用细胞角蛋白的免疫细胞化学方法及自动显微图像对比检测分析。结果:根治术前后骨髓中微转移肿瘤细胞检出率密切相关(P<0.005),术前肿瘤细胞总检出率为31.4%,术后为34.3%,无显著性差别(P=0.65);三种泌尿肿瘤患者根治术前后检出率比较亦均无明显差别(P=0.70P=0.10P=0.59) 但术后检出的肿瘤细胞数多于术前者总数分别为64和44,3个以上阳性细胞仅在术后出现,两次阳性的细胞数均值分别为1.31±0.48和1.95±1.02(P=0.009 9)12例患者手术8天后检出细胞数(22)明显多于术前者(7),P=0.01。结论:免疫细胞化学方法可作为检测骨髓中隐蔽的微转移肿瘤细胞的敏感标准方法,根治术前后检出率虽无明显改变 但术后及手术8天以后检出细胞数明显多于术前者,提示手术可能会促使肿瘤细胞在骨髓中的播散。 | 黄瑜峰 刘立义 Riesenberg R Oberneder R Buchner A Hofstetter A 李雅玲 | 2001 | 中国肿瘤临床2001，28，1： | 1 |
| 11 | Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli 显示文摘 | Buchner J Rudolph R | 1991 | Biotechnology1991，9，2： | 1 |
| 12 | Heat shock protein 27 as a prognostic and predictive biomarker in pancreatic ductal adenocarcinoma显示文摘 | Claus Sch?fer Hendrik Seeliger Dominik C. Bader Gerald Assmann Denise Buchner Yang Guo Andreas Ziesch Andreas Palagyi Stephanie Ochs Rüdiger P. Laubender Andreas Jung Enrico N. De Toni Thomas Kirchner Burkhard G?ke Christiane Bruns Eike Gallmeier | 2012 | Journal of Cellular and Molecular Medicine2012，，8： | 1 |
| 13 | Interactions and dy- namics in ionic liquids 显示文摘 | STOPPA A HUNGER J BUCHNER R | 2008 | J Phys Chem B2008，112，： | 1 |
| 14 | A generalization of blind source separation algorithms for convolutive mixtures based on second-order statistics 显示文摘 | Buchner H Aichner R and Kellermann W | 2005 | IEEE Transactions on Speech and Audio Processing2005，13，1： | 1 |
| 15 | Conductivities of binary mixtures of ionic liquids with polar solvents 显示文摘 | Stoppa A Hunger J Buchner R | 2009 | J Chem Eng Data2009，54，： | 1 |
| 16 | Human Toll-like receptor 2 mediates induction of the antimicrobial peptide human beta defensin 2 in response to bacterial lipoprotein显示文摘 | BIRCHLER T SEIBL R BUCHNER K | 2001 | Eur J Immunol2001，31，： | 1 |
| 17 | International Conference for the Development of a Consensus on the Manage?ment and Prevention of Severe Candidal Infections显示文摘 | EdwardsJ Bodey G Pv Bowden R Av Buchner T de Pauw BE Filler S G | 1997 | Clin Infect Dis1997，25，： | 1 |
| 18 | Improved catalytic performance of hierarchical ZSM-5 synthesized by desilication with surfactants 显示文摘 | SCHMIDTF LOHE M R BUCHNER B | 2013 | Microporous and Mesoporous Materials2013，165，1： | 1 |
| 19 | Human Toll-like receptor 2 mediates induction of the antimicrobial peptide human beta-defensin 2 in response to bacterial lipoprotein 显示文摘 | Birchler T Seibl R Buchner K | 2001 | Eur J Immuno2001，31，11： | 1 |
| 20 | A gener- alization of blind source separation algorithms for convolu- tive mixtures based on second-order statistics显示文摘 | BUCHNER H AICHNER R KELLERMANN W | 2005 | IEEE Transactions on Speech and Audio Processing2005，13，1： | 1 |
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