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| 1 | Effect of alcohol exposure on hepatic superoxide generation and hepcidin expression显示文摘AIM: To understand the role of mitochondrial-produced superoxide(O 2 ?) in the regulation of iron-regulatory hormone, hepcidin by alcohol in the liver. METHODS: For alcohol experiments, manganese superoxide dismutase knockout mice heterozygous for Sod2 gene expression(Sod2 +/) and age-matched littermate control mice(LMC), expressing Sod2 gene on both alleles, were exposed to either 10%(w/v) ethanol in the drinking water or plain water(control) for 7 d. Total cellular O 2 ? levels in hepatocytes isolated from the livers of mice were measured by electron paramagnetic resonance spectroscopy. The mitochondrial-targeted, O 2 ?-sensitive fluorogenic probe, MitoSOX Red and flow cytometry were utilized to measure O 2 ? in mitochondria. Gene and protein expression were determined by Taqman Real-time quantitative PCR and Western blotting, respectively. RESULTS: Sod2 +/- mice expressed 40% less MnSOD protein(SOD2) in hepatocytes compared to LMC mice. The deletion of Sod2 allele did not alter the basal expression level of hepcidin in the liver. 10% ethanol exposure for 1 wk inhibited hepatic hepcidin mRNA expression three-fold both in Sod2 +/ and LMC mice. O 2 ? levels in hepatocytes of untreated Sod2 +/ mice were three-fold higher than in untreated LMC mice, as observed by electron paramagnetic resonance spectroscopy. O 2 ? levels in mitochondria of Sod2 +/ mice were four-fold higher than in mitochondria of untreated LMC mice, as measured by MitoSOX Red fluorescence and flow cytometry. Alcohol induced a two-fold higher increase in O 2 ? levels in hepatocytes of LMC mice than in Sod2 +/ mice compared to respective untreated counterparts. In contrast, 1 wk alcohol exposure did not alter mitochondrial O 2 ? levels in both Sod2 +/- and control mice. CONCLUSION: Mitochondrial O2 ? is not involved in the inhibition of liver hepcidin transcription and thereby regulation of iron metabolism by alcohol. These findings also suggest that short-term alcohol consumption significantly elevates O 2 ? levels in hepatocytes, which appears not to originate from mitochondria. | Duygu Dee Harrison-Findik Sizhao Lu Emily M Zmijewski Jocelyn Jones Matthew C Zimmerman | 2013 | World Journal of Biological Chemistry2013，4，4： | 2 |
| 2 | Taxa- Specific Changes in Soil Microbial Community Composition Induced by Pyrogenic Carbon Amendments 显示文摘 | KHODADAD C L M ZIMMERMAN A R GREEN S J | 2011 | Soil Biology and Biochemistry2011，43，2： | 1 |
| 3 | Structural damage assessment using a generalized minimum rank perturbation theory显示文摘 | Zimmerman D C | 1994 | AIAA Journal1994，32，4： | 1 |
| 4 | Boundary element solution of 3-D wave scatter problems in a poroelastic medium显示文摘 | Zimmerman C Stern M | 1993 | Eng Anal Bound Elem1993，12，： | 1 |
| 5 | Lyn kinase-dependent regulation of miR181 and myeloid cell leukemia-1 expression:implications for drug resistance in myelogenous leukemia显示文摘 | ZIMMERMAN E I DOLLINS C M CRAWFORD M | | 0，，05： | 1 |
| 6 | Mathews Formation and activation by phosphorylation of activin receptor complexes Mol显示文摘 | S A Willis C M Zimmerman | 1996 | Endocrinol1996，10，： | 1 |
| 7 | Deep inelastic separated response functions from 40 Ca, and 48Ca显示文摘 | Deady M Williamson C F Zimmerman P D | 1986 | Phys Rev C1986，33，： | 1 |
| 8 | Influence of surface texture and charge on the biocompatibility of endovascular stents显示文摘 | Zimmerman M Metz J | 1995 | Coronary Artery Dis1995，6，： | 1 |
| 9 | Heart block and empirical therapy after transcatheter closure of perimembranous ventricular septal defect 显示文摘 | YIP W C ZIMMERMAN F HIJAZI Z M | 2005 | Catheter Cardiovasc Interv2005，66，3： | 1 |
| 10 | Neuronal uptake and intracellular superoxide scavenging of a fullerene (C60) poly(2-oxazoline) s nanoformulation 显示文摘 | Tong J Zimmerman M C Li S Yi X lmxenhofer R Jordan R | 2011 | Biomaterials2011，32，： | 1 |
| 11 | An inverse methodology for the rheology of a power-law non-Newtonian fluid显示文摘 | BANDULASENA H C H ZIMMERMAN W B REES J M | 2008 | Journal of Mechanical Engineering Science2008，222，5： | 1 |
| 12 | Correcting finite element modes using a symmetric eigenstructure assignment technique显示文摘 | Zimmerman D C Widengren M | 1990 | AIAA J1990，28，9： | 1 |
| 13 | Boundary element solution of 3-D wave scatter problems in a poroelastic medium显示文摘 | ZIMMERMAN C STERN M | 1993 | Engineering Analysis with Boundary Elements1993，12，4： | 1 |
| 14 | Boundary element solution of 3-D wave scatter problems in a poroelastic medium显示文摘 | ZIMMERMAN C STERN M | 1993 | Engineering Analysis with Boundary Elements1993，12，： | 1 |
| 15 | Structural Damage Detection in Beam Structures Using a Subspace Rotation Algorithm显示文摘 | Zimmerman D C Kaouk M | 1992 | AIAA Journal1992，34，12： | 1 |
| 16 | Variability of total and pathogenic Vibrioparahaemolyticus densities显示文摘 | ZIMMERMAN A M DEPAOLA A BOWERS J C | 2007 | Applied and Environmental Microbiology2007，73，23： | 1 |
| 17 | Tailoring mixed matrix composite membranes for gas separations 显示文摘 | Zimmerman C M Singh A Koros W J | 1997 | J Membr Sci1997，137，： | 1 |
| 18 | Correcting Finite Element Modes using a Symmetric Eigenstructure Assignment Technique显示文摘 | Zimmerman D C and Widengren M | 1990 | AIAA J1990，28，9： | 1 |
| 19 | Structural damage detection using a minimum rank update theory 显示文摘 | ZIMMERMAN D C KAOUK M | 1994 | Journal of Vibration and Acoustics1994，116，2： | 1 |
| 20 | Synthetic Hosts by Monomolecular hnprinting Inside Dendrimers 显示文摘 | Zimmerman S C Wendland M S Rakow N A | 2002 | Nature2002，418，： | 1 |
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