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| 1 | Vacuum membrane distillation processes for aqueous solution treatment-A review显示文摘 | Chiam C K Sarbatly R | 2013 | Chem Eng Processing: Process Intensification2013，74，8： | 1 |
| 2 | Serum outperforms plasma in small extracellular vesicle microRNA biomarker studies of adenocarcinoma of the esophagus显示文摘BACKGROUND Circulating microRNAs(miRNAs)are potential biomarkers for many diseases.However,they can originate from non-disease specific sources,such as blood cells,and compromise the investigations for miRNA biomarkers.While small extracellular vesicles(sEVs)have been suggested to provide a purer source of circulating miRNAs for biomarkers discovery,the most suitable blood sample for sEV miRNA biomarker studies has not been defined.AIM To compare the mi RNA profiles between matched serum and plasma s EV preparations to determine their suitability for biomarker studies.METHODS Matched serum and plasma samples were obtained from 10 healthy controls and10 patients with esophageal adenocarcinoma.s EV isolates were prepared from serum and plasma using Exo Quick TM and quantified using Nano Sight.RNA was extracted from s EV preparations with the mi RNeasy Serum/Plasma kit and profiled using the Taqman Openarray q PCR.The overall mi RNA content and theexpression of specific mi RNAs of reported vesicular and non-vesicular origins were compared between serum and plasma s EV preparations.The diagnostic performance of a previously identified multi-mi RNA biomarker panel for esophageal adenocarcinoma was also compared.RESULTS The overall mi RNA content was higher in plasma s EV preparations(480 mi RNAs)and contained 97.5%of the mi RNAs found in the serum s EV preparations(412 mi RNAs).The expression of commonly expressed mi RNAs was highly correlated(Spearman’s R=0.87,P<0.0001)between the plasma and serum s EV preparations,but was consistently higher in the plasma s EV preparations.Specific blood-cell mi RNAs(hsa-mi R-223-3 p,hsa-mi R-451 a,mi R-19 b-3 p,hsa-mi R-17-5 p,hsa-mi R-30 b-5 p,hsa-mi R-106 a-5 p,hsa-mi R-150-5 p and hsa-mi R-92 a-3 p)were expressed at 2.7 to 9.6 fold higher levels in the plasma s EV preparations compared to serum s EV preparations(P<0.05).In plasma s EV preparations,the percentage of protein-associated mi RNAs expressed at relatively higher levels(Ct 20-25)was greater than serum s EV preparations(50%vs 31%).While the percentage of vesicle-associated mi RNAs expressed at relatively higher levels was greater in the serum s EV preparations than plasma s EV preparations(70%vs 44%).A 5-mi RNA biomarker panel produced a higher cross validated accuracy for discriminating patients with esophageal adenocarcinoma from healthy controls using serum s EV preparations compared with plasma s EV preparations(AUROC 0.80 vs 0.54,P<0.05).CONCLUSION Although plasma s EV preparations contained more mi RNAs than serum s EV preparations,they also contained more mi RNAs from non-vesicle origins.Serum appears to be more suitable than plasma for s EV mi RNAs biomarkers studies. | Karen Chiam George C Mayne Tingting Wang David I Watson Tanya S Irvine Tim Bright Lorelle T Smith Imogen A Ball Joanne M Bowen Dorothy M Keefe Sarah K Thompson Damian J Hussey | 2020 | World Journal of Gastroenterology2020，26，20： | 1 |
| 3 | Balancing exploration and exploitation with adaptive variation for evolutionary multiobjective optimization显示文摘 | TAN K C CHIAM S C MAMWN A A | 2009 | European Journal of Operational Research2009，197，2： | 1 |
| 4 | ReSTOR intraocular lens implantation in cataract surgery :quality of vision显示文摘 | Chiam P J Chan J H Aggarwal R K | 2006 | J Cataract Refract Surg2006，32，9： | 1 |
| 5 | A competitive and cooperative co-evolutionary approach to multi-objective particle swarm optimization algorithm design 显示文摘 | C K Goh K C Tan D S Liu S C Chiam | 2010 | European Jourllal of Operational Research2010，202，1： | 1 |
| 6 | Survey of common practices among oeulofaeial surgeons in the Asia-Pacific region: Graves' orbitopathy显示文摘 | Sundar G Chiam N Lun K | 2014 | Orbit2014，33，2： | 1 |
| 7 | Circulating serum exosomal mirnas as potential biomarkers for esophageal adenocarcinoma显示文摘 | Chiam K Wang T Watson DI | 2015 | J Gas- trointest Surg2015，19，7： | 1 |
| 8 | Vacuum membrane distillation processes for aqueous solution treatment A review 显示文摘 | CHIAM C K SARBATLY R | 2013 | Chemical Engineering and Processing2013，，74： | 1 |
| 9 | GSTP1 DNA methylation and expression status is indicative of 5-aza-2'-deoxycytidine efficacy in human prostate cancer cells显示文摘 | CHIAM K CENTENERA M M BUTLER LM | 2011 | PIoS One2011，6，25： | 1 |
| 10 | ReSTOR intraocular lens implantation in cataract surgery : quality of vision显示文摘 | Chiam P J Chan J H Aggarwal R K | 2006 | J Cataract Refract Surg2006，32，： | 1 |
| 11 | GSTP1 DNA methylation and expression status is indicative of 5-aza-2'-deoxycytidine efficacy in human prostate cancer cells显示文摘 | Chiam K Centenera MM Butler LM | | 0，，09： | 1 |
| 12 | Formation of Nickel Oxide Nanotubes with Uniform Wall Thickness by Low-temperature Thermal Oxidation Through Understanding the Limiting Effect of Vacancy Diffusion and the Kirkendall Phenomenon显示文摘 | Ren Y Chim W K Chiam S Y | 2012 | Adv Funct Mater2012，20，： | 1 |
| 13 | Purification of aquacultural water: Conventional and new membrane-based techniques 显示文摘 | Chiam C K Sarbatly R | 2011 | Sep Purif Rev2011，40，2： | 1 |
| 14 | Formation of Nickel Oxide Nanotubes with Uniform wall Thickness by Low - temperature Ther- mal Oxidation through Understanding the Limiting Effect of Vacancy Dif- fusion and the Kirkendall Phenomenon 显示文摘 | Ren Y Chim W K Chiam S Y | 2010 | Adv Funct Mater2010，20，： | 1 |
| 15 | Comparative analysis and study of ionized metal plasma (IMP)-Cu and chemical vapor deposition (CVD)-Cu on diffusion barrier properties of IMP-TaN on SiO2显示文摘 | LEE Y K KHIN M L KIM J H THOMAS O CHIAM S Y LEE K | 2000 | Materials Science and Engineering B2000，77，： | 1 |
| 16 | Formation of Nickel Oxide Nanotubes with Uniform wall Thickness by Low-temperature Thermal Oxidation Through Understanding the Limiting Effect of Vacancy Diffusion and Kirkendall Phenomenon显示文摘 | Ren Y Chim W K Chiam S Y | 2010 | Adv Funct Mater2010，20，： | 1 |
| 17 | Investigation of silicon diffusion into yttrium using X-ray photoelectron spectroscopy 显示文摘 | Chiam S Y Chim W K Huan A C H Pan J S | 2006 | Applied Physics Letters2006，88，01： | 1 |
| 18 | ReSTOR intraocular lens implantation in cataract surgery:quality of vision显示文摘 | CHIAM P J CHAN J H AGGARAL P K | 2006 | J Cataract Refract Surg2006，32，9： | 1 |
| 19 | Separation of deformable particles in deterministic lateral displacement devices 显示文摘 | QUEK R LE D V CHIAM K H | 2011 | Physical Review E2011，83，56： | 1 |
| 20 | Balancing exploration and exploitation with adaptive variation for evolutionary muhi-objective optimization 显示文摘 | Tan K C Chiam S C Mamun A A | 2009 | European Journal of Operational Research2009，197，2： | 1 |
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