|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Nat Biotechnol:将人星形胶质细胞重编程为多巴胺能神经元,有助治疗帕金森病显示文摘帕金森病是一种主要影响运动系统的神经退行性疾病。它的特征在于大脑中的多巴胺能神经元(dopaminergic neuron)渐进性丧失。尽管当前的疗法旨在补充多巴胺水平,但是没有一种疗法能够恢复这些丢失的细胞。如今。在一项新的研究中,来自瑞典、奥地利、西班牙和美国的研究人员开发出一种方法:将神经胶质细胞(glialcell)转化为活性的多巴胺能神经元,并且所产生的多巴胺能神经元能够部分恢复帕金森病模式小鼠的运动功能。这项概念验证研究可能为开发出一种治疗这种疾病的新方法铺平道路。 | Pia Rivetti di Val Cervo, Elisa Martín-Montañez, Enrique M Toledo, Gioele La Manno, Sara Padrell Sánchez, Sten Linnarsson Ernest Arenas Roman A Romanov, Christian Pifl Tibor Harkany Roman A Romanov, Giada Spigolon, Débora Masini, Michael Feyder, Tibor Harkany Gilberto Fisone Elisa Martín-Montañez Yi-Han Ng Marius Wernig | 2017 | 现代生物医学进展2017，17，17： | 11 |
| 2 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 3 | Combination of corticosteroids and 5-aminosalicylates or corticosteroids alone for patients with moderate-severe active ulcerative colitis:A global survey of physicians'practice显示文摘AIM To examine treatment decisions of gastroenterologists regarding the choice of prescribing 5-aminosalycilates(5ASA) with corticosteroids(CS) versus corticosteroids alone for patients with active ulcerative colitis(UC). METHODS A cross-sectional questionnaire exploring physicians' attitude toward 5ASA + CS combination therapy vs CS alone was developed and validated. The questionnaire was distributed to gastroenterology experts in twelve countries in five continents. Respondents' agreement with stated treatment choices were assessed by standardized Likert scale. Background professional characteristics of respondents were analyzed for correlation with responses. RESULTS Six hundred and sixty-four questionnaires were distributed and 349 received(52.6% response rate). Of 340 eligible respondents, 221(65%) would continue 5ASA in a patient hospitalized for intravenous CS treatment due to a moderate-severe UC flare, while 108(32%) would stop the 5ASA(P < 0.001), and 11(3%) are undecided. Similarly, 62% would continue 5ASA in an out-patient starting oral CS. However, only 140/340(41%) would proactively start 5ASA in a hospitalized patient not receiving 5ASA before admission. Most(94%) physicians consider the safety profile of 5ASA as very good. Only 52% consider them inexpensive, 35% perceive them to be expensive and 12% are undecided. On multi-variable analysis, less years of practice and perception of a plausible additive mechanistic effect of 5ASA + CS were positively associated with the decision to continue 5ASA with CS. CONCLUSION Despite the absence of data supporting its benefit, most gastroenterologists endorse combination of 5ASA + CS for patients with active moderate-to-severe UC. Randomized controlled trials are needed to assess if 5ASA confer any benefit for these patients. | Shomron Ben-Horin Jane M Andrews Konstantinos H Katsanos Florian Rieder Flavio Steinwurz Konstantinos Karmiris Jae Hee Cheon Gordon William Moran Monica Cesarini Christian D Stone Doron Schwartz Marijana Protic Xavier Roblin Giulia Roda Min-Hu Chen Ofir Har-Noy Charles N Bernstein | 2017 | World Journal of Gastroenterology2017，23，16： | 3 |
| 4 | Multiband emission from single β-NaYF_(4)(Yb,Er) nanoparticles at high excitation power densities and comparison to ensemble studies显示文摘Ensemble and single particle studies of the excitation power density (P)-dependent upconversion luminescence (UCL) of core and core-shell β-NaYF_(4):Yb,Er upconversion nanoparticles (UCNPs) doped with 20% Yb^(3+) and 1% or 3% Er^(^(3+)) performed over a P regime of 6 orders of magnitude reveal an increasing contribution of the emission from high energy Er^(3+) levels at P > 1 kW/cm^(2). This changes the overall emission color from initially green over yellow to white. While initially the green and with increasing P the red emission dominate in ensemble measurements at P < 1 kW/cm^(2), the increasing population of higher Er^(^(3+)) energy levels by multiphotonic processes at higher P in single particle studies results in a multitude of emission bands in the ultraviolet/visible/near infrared (UV/vis/NIR) accompanied by a decreased contribution of the red luminescence. Based upon a thorough analysis of the P-dependence of UCL, the emission bands activated at high P were grouped and assigned to 2–3, 3–4, and 4 photonic processes involving energy transfer (ET), excited-state absorption (ESA), cross-relaxation (CR), back energy transfer (BET), and non-radiative relaxation processes (nRP). This underlines the P-tunability of UCNP brightness and color and highlights the potential of P-dependent measurements for mechanistic studies required to manifest the population pathways of the different Er^(3+) levels. | Florian Frenzel Christian Würth Oleksii Dukhno Frédéric Przybilla Lisa MWiesholler Verena Muhr Thomas Hirsch Yves Mély Ute Resch-Genger | 2021 | Nano Research2021，14，11： | 3 |
| 5 | Serotonin type 3 receptor subunit gene polymorphisms associated with psychosomatic symptoms in irritable bowel syndrome:A multicenter retrospective study显示文摘BACKGROUND Single-nucleotide polymorphisms(SNPs)of the serotonin type 3 receptor subunit(HTR3)genes have been associated with psychosomatic symptoms,but it is not clear whether these associations exist in irritable bowel syndrome(IBS).AIM To assess the association of HTR3 polymorphisms with depressive,anxiety,and somatization symptoms in individuals with IBS.METHODS In this retrospective study,623 participants with IBS were recruited from five specialty centers in Germany,Sweden,the United States,the United Kingdom,and Ireland.Depressive,anxiety,and somatization symptoms and sociodemographic characteristics were collected.Four functional SNPs—HTR3A c.-42C>T,HTR3B c.386A>C,HTR3C c.489C>A,and HTR3E c.*76G>A—were genotyped and analyzed using the dominant and recessive models.We also performed separate analyses for sex and IBS subtypes.SNP scores were calculated as the number of minor alleles of the SNPs above.The impact of HTR3C c.489C>A was tested by radioligand-binding and calcium influx assays.RESULTS Depressive and anxiety symptoms significantly worsened with increasing numbers of minor HTR3C c.489C>A alleles in the dominant model(F_(depressive)=7.475,P_(depressive)=0.006;F_(anxiety)=6.535,P_(anxiety)=0.011).A higher SNP score(range 0-6)was linked to a worsened depressive symptoms score(F=7.710,P-linear trend=0.006)in IBS.The potential relevance of the HTR3C SNP was corroborated,showing changes in the expression level of 5-HT3AC variant receptors.CONCLUSION We have provided the first evidence that HTR3C c.489C>A is involved in depressive and anxiety symptoms in individuals with IBS.The SNP score indicated that an increasing number of minor alleles is linked to the worsening of depressive symptoms in IBS. | Sabrina Berens Yuanjun Dong Nikola Fritz Jutta Walstab Mauro D'Amato Tenghao Zheng Verena Wahl Felix Boekstegers Justo Lorenzo Bermejo Cristina Martinez Stefanie Schmitteckert Egbert Clevers Felicitas Engel Annika Gauss Wolfgang Herzog Robin Spiller Miriam Goebel-Stengel Hubert Mönnikes Viola Andresen Frieling Thomas Jutta Keller Christian Pehl Christoph Stein-Thöringer Gerard Clarke Timothy G Dinan Eamonn M Quigley Gregory Sayuk Magnus Simrén Jonas Tesarz Gudrun Rappold Lukas van Oudenhove Rainer Schaefert Beate Niesler | 2022 | World Journal of Gastroenterology2022，28，21： | 2 |
| 6 | A vital sugar code for ricin toxicity显示文摘 | Jasmin Taubenschmld Johannes Stadlmann Markus Jost Tove Irene Klok.k Cory D Rillahan Andreas Leibbrandt Karl Mechtler James C Paulson Julian Jude Johannes Zuber Kirsten Sandvig Ulrich Elling Thorsten Marquardt Christian Thiel Christian Koerner Josef M Penninger | 2017 | Cell Research2017，27，11： | 2 |
| 7 | Neurotopographic considerations in microsurgical treatment of small acoustic neurimomas显示文摘 | Wolfgang TK J diaz D Christian M | 1998 | Neurosurg1998，88，4： | 1 |
| 8 | Ship routing and scheduling: status and perspectives 显示文摘 | CHRISTIAN M FAGERHOLT K RONEN D | 2004 | Transportation Sci2004，38，1： | 1 |
| 9 | Engagement and Achievements: A Case Study of Design - Based Learning in a Sci- ence Context显示文摘 | Yaron Doppclt Matthew M Mchah Christian D Schunn | 2008 | Journal of Technology Education2008，19，2： | 1 |
| 10 | NMR study of cyclodextrin inclusion of fluorocarbon surfactants in solution显示文摘 | GUO W FUNG B M CHRISTIAN S D | 1992 | Langmuir1992，8，2： | 1 |
| 11 | The use of poller screws for metaphyseal tibia and femur fractures treated with small2 diameter in2 tramedullary nails 显示文摘 | CHRISTIAN M THOMAS M D STEFAN D | 2004 | Orthopaedics2004，18，4： | 1 |
| 12 | Recycling of magnesium alloy: chemical equilibria between magnesium-lithium-based melts and salt melts显示文摘 | KLAUS S CHRISTIAN T M ANDRE D | 2002 | Metallurgical and Materials Transactions A2002，33，： | 1 |
| 13 | Primary Gastric B-Cell Lymphoma: Results of a Prospective Multicenter Study显示文摘 | Wolfgang Fischbach* Brigitte Dragosics? Maria-Elisabeth Kolve-Goebeler§ Christian Ohmann∥ Axel Greiner? Qin Yang∥ Stephan B?hm? Patrick Verreet∥ Olaf Horstmann# Martin Busch** Eckhart Dühmke** Hans-Konrad Müller-Hermelink? Klaus Wilms§ | 2000 | Gastroenterology2000，，5： | 1 |
| 14 | Gene expression profiling of 3T3-L1 adipocytes exposed to phloretin显示文摘 | Meryl H Claire E Y Christiane M D | 2010 | J Nutr Biochem2010，21，7： | 1 |
| 15 | Embolit pneumopathy induced by oleic acid显示文摘 | Christian M D Danielle J D | 1997 | Am J Pathol1997，87，： | 1 |
| 16 | Derivatives of γ- Valerolactone, 1,4-Pentanediol and 1,4-Di-( β-cyanoe- thoxy)-Pentane 显示文摘 | Christian R V Brown H D Hixon R M | 1947 | Journal of the American' Chemical Society1947，69，： | 1 |
| 17 | Processing Snow for High Strength Roads and Runways 显示文摘 | RENEE M LANG GEORGE L BLAISDELL CHRISTIAN D'URSO | 1997 | Cold Regions Science and Technology1997，25，： | 1 |
| 18 | Effective primary isolation of wild-type Canine distemper virus in MDCK, MV1 Lu and Vero cells without nucleotide sequence changes within the entire haemagglutinin protein gene and in subgenomic sections of the fusion and phospho protein genes显示文摘 | John A Lednicky Thomas P Meehan Michael J Kinsel Jean Dubach Laura L Hungerford Nicolene A Sarich Kelley E Witecki Michael D Braid Casandra Pedrak Christiane M Houde | 2004 | Journal of Virological Methods2004，，2： | 1 |
| 19 | An Improved Optimization Algorithm and a Bayes Factor Termination Criterion for Sequential Projection Pursuit显示文摘 | Bobbie J M Webb-Robertson Kristin H J Scott D H Christian P Bob W W | 2005 | Chemometrics and Intelligence Laboratory Systems2005，77，12： | 1 |
| 20 | Sperm cells of Zea mays have a complex complement of mRNAs显示文摘 | Engel M L Annie C Christian D | 2003 | Plant Journal2003，34，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员