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| 1 | Black soldier fly and gut health in broiler chickens:insights into the relationship between cecal microbiota and intestinal mucin composition显示文摘Background:The relationship between diet and intestinal microbiota and mucin composition appears to be fundamental for poultry gut health.The effects of insect meal(whose role as alternative feed ingredient is now well recognized)on gut microbiota and mucin composition have recently been reported in Tenebrio molitor-fed free-range and broiler chickens,but no data are currently available for Hermetia illucens(HI)-fed broilers.The present study evaluated the effects of dietary HI meal inclusion on cecal microbiota and intestinal mucin composition of broiler chickens.Results:A total of 256 male broiler chickens were allotted to 4 dietary treatments(control diet[C]and 5%,10%and 15%HI meal inclusion,with 8 replicate pens/treatment and 8 birds/pen)and slaughtered at 35 d of age(2 animals/pen,16 birds/diet).The cecal microbiota assessment by 16S rRNA amplicon based sequencing showed lower alpha diversity in HI15 chickens(Shannon,P<0.05)and higher beta diversity(Adonis and ANOSIM,P<0.001)in birds fed HI diets than C.Furthermore,HI15 birds displayed significant increase of the relative abundance of Proteobacteria phylum(False Discovery Rate[FDR]<0.05)when compared to HI10.L-Ruminococcus(Ruminococcus from Lachnospiraceae family),Faecalibacterium,Blautia and Clostridium genera were found to be characteristic of HI5 cecal microbiota(FDR<0.05),while broiler chickens fed HI10 and HI15 diets were characterized(FDR<0.05)by Lactobacillus and Ruminococcus(HI10)and Bacteroides,Roseburia and Helicobacter genera(HI15).Periodic-acid Schiff,Alcian Blue pH 2.5 and high iron diamine staining on small and large intestine also demonstrated lower mucin staining intensity in the intestinal villi of HI10 and HI15 birds than C(P<0.05).Conclusions:Dietary HI meal utilization at low inclusion levels(i.e.,5%)positively influenced either the cecal microbiota or the gut mucin dynamics in terms of selection of potentially beneficial bacteria and increase in villi mucins.However,high inclusion levels(in particular the 15%)may have a negative influence in terms of partial reduction of microbial complexity,reduction of potentially beneficial bacteria,selection of bacteria with mucolytic activity and decrease in villi mucins. | Ilaria Biasato Ilario Ferrocino Sihem Dabbou Rocchina Evangelista Francesco Gai Laura Gasco Luca Cocolin Maria Teresa Capucchio Achille Schiavone | 2020 | Journal of Animal Science and Biotechnology2020，11，3： | 4 |
| 2 | Lac-tic acid bacteria ecology of three traditional fermented sau-sages produced in the North of Italy as determined by mo-lecular methods显示文摘 | Luca Cocolin Paola Dolci Kalliopi Rantsiou | 2009 | Meat Science2009，82，1： | 1 |
| 3 | Temperature gradient gel electrophoresis of the amplified product of a small 16S rRNA gene fragment for the identification of Listeria species isolated from food 显示文摘 | MANZANO MARISA COCOLIN LUCA CANTONI CARLO | 2000 | J Food Protect2000，63，5： | 1 |
| 4 | Lactic acid bacteria ecology of three traditional fermented sausages produced in the North of Italy as determined by molecular methods显示文摘 | Luca Cocolin Paola Dolci Kalliopi Rantsiou Rosalinda Urso Carlo Cantoni Giuseppe Comi | 2009 | Meat Science2009，，1： | 1 |
| 5 | Technological characterization of bacteriocin producing Lactococcus lactis strains employed to control Listeria monocytogenes in Cottage cheese显示文摘 | BARBARA DAL BELLO LUCA COCOLIN GIUSEPPE ZEPPA | 2012 | International Journal of Food Microbiology2012，，153： | 1 |
| 6 | Effects of dietary Hermetia illucens meal inclusion on cecal microbiota and small intestinal mucin dynamics and infiltration with immune cells of weaned piglets显示文摘Background: The constant interaction between diet and intestinal barrier has a crucial role in determining gut health in pigs. Hermetia illucens(HI) meal(that represents a promising, alternative feed ingredient for production animals) has recently been demonstrated to influence colonic microbiota, bacterial metabolite profile and mucosal immune status of pigs, but no data about modulation of gut mucin dynamics are currently available. The present study evaluated the effects of dietary HI meal inclusion on the small intestinal mucin composition of piglets, as well as providing insights into the cecal microbiota and the mucosal infiltration with immune cells.Results: A total of 48 weaned piglets were randomly allotted to 3 dietary treatments(control diet [C] and 5% or10% HI meal inclusion [HI5 and HI10], with 4 replicate boxes/treatment and 4 animals/box) and slaughtered after61 days of trial(3 animals/box, 12 piglets/diet). The cecal microbiota assessment by 16 S r RNA amplicon based sequencing showed higher beta diversity in the piglets fed the HI-based diets than the C(P < 0.001). Furthermore,the HI-fed animals showed increased abundance of Blautia, Chlamydia, Coprococcus, Eubacterium, Prevotella,Roseburia, unclassified members of Ruminococcaceae, Ruminococcus and Staphylococcus when compared to the C group(FDR < 0.05). The gut of the piglets fed the HI-based diets showed greater neutral mucin percentage than the C(P < 0.05), with the intestinal neutral mucins of the HI-fed animals being also higher than the sialomucins and the sulfomucins found in the gut of the C group(P < 0.05). Furthermore, the piglets fed the HI-based diets displayed lower histological scores in the jejunum than the other gut segments(ileum [HI5] or ileum and duodenum [HI10], P < 0.05).Conclusions: Dietary HI meal utilization positively influenced the cecal microbiota and the small intestinal mucin dynamics of the piglets in terms of selection of potentially beneficial bacteria and preservation of mature mucin secretory architecture, without determining the development of gut inflammation. These findings further confirm the suitability of including insect meal in swine diets. | Ilaria Biasato Ilario Ferrocino Elena Colombino Francesco Gai Achille Schiavone Luca Cocolin Valeria Vincenti Maria Teresa Capucchio Laura Gasco | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 1 |
| 7 | Dried fruit pomace inclusion in poultry diet:growth performance, intestinal morphology and physiology显示文摘Background: Fruit pomaces are by-products rich in polyphenol compounds and dietary fiber. They seem to play an important role in regulating the gut microbiota, morphology and physiology. The aim of this study was to assess whether apple(A), blackurrant(B) or strawberry(S) pomaces could be suitable ingredients in broiler diets and their effect on gut health. A total of 480 male broilers were randomly allotted to 8 dietary treatments with lower(3%-L) or higher(6%-H) dietary fiber content: two control groups(CL/CH), two A diets(AL/AH), two B diets(BL/BH), two S diets(SL/SH). Diet and fruit pomaces were chemically analyzed to assess polyphenol concentration and fibre fraction content. After the evaluation of growth performance, 6 birds/group were slaughtered at 35 days of age. Morphometric and histopathological investigations were performed on duodenum, jejunum and ileum.Excreta were collected to perform microbiota evaluation by 16 S DNA sequencing. Weight, viscosity, enzymatic activity, short chain fatty acid(SCFAs) and ammonia concentration were determined in ileum and/or ceca content.Results: A pomace and A diets showed the lowest polyphenol content and the highest content of soluble fibre fraction. No significant differences were observed for growth performance, gut morphometry and histopathology(P > 0.05). Dietary fruit pomace inclusion increased the weight of ileum and ceca and the ileum digesta viscosity(P < 0.05). In the ileum, A and S groups showed lower bacterial α-glucosidase activity than C groups. Moreover,small intestine SCFAs concentration was higher in fruit pomaces diets(P < 0.05). In ceca, B and S groups showed lower ammonia concentration and higher SCFAs than C. Dietary treatments also influenced the activity of α-glucosidase, α-galactosidase, β-galactosidase β-glucuronidase and xylase. Regarding microbiota, at phylum level,Firmicutes were differentially abundant across treatment(maximum for C and minimum in S, FDR > 0.05). At genus level, an increase of Weissella in AH and Erwinia in S/B diets, as well as a decrease of Lactobacillus in all fruit pomace groups were recorded(P < 0.05).Conclusions: Fruit pomaces could be suitable ingredients in poultry nutrition even if further studies are needed to better understand which doses is more recommended to avoid negative effects on gut microbiota. | Elena Colombino Ilario Ferrocino Ilaria Biasato Luca Simone Cocolin Daniel Prieto-Botella Zenon Zduńczyk Jan Jankowski Joanna Milala Monika Kosmala Bartosz Fotschki Maria Teresa Capucchio Jerzy Juśkiewicz | 2020 | Journal of Animal Science and Biotechnology2020，11，4： | 1 |
| 8 | Characterization of Kloeckera apiculata strains from the Friuli region in Northern Italy 显示文摘 | Giuseppe Comi Patrizia Romano Luca Cocolin | 2001 | Word Journal of Microbiology & Biotechnology2001，17，： | 1 |
| 9 | Fermented Meat Products显示文摘 | Kalliopi Rantsiou Luca Cocolin | 2008 | Molecular Techniques in the Microbial Ecology of Fermented Foods2008，，： | 1 |
| 10 | The rpoB gene as a target for PCR-DGGE analysis to follow lactic acid bacterial population dynamics during food fermentations显示文摘 | Kalliopi Rantsiou Giuseppe Comi Luca Cocolin | 2004 | Food Microbiology2004，21，： | 1 |
| 11 | Lactic acid bacteria ecology of three traditional fermented sausages produced in the North of Italy as determined by molecular methods显示文摘 | Luca Cocolin Paola Dolci Kalliopi Rantsiou Rosalinda Urso Carlo Cantoni Giuseppe Comi | 2009 | Meat Science2009，，1： | 1 |
| 12 | Biodiversity and dynamics of meat fermentations: The contribution of molecular methods for a better comprehension of a complex ecosystem显示文摘 | Luca Cocolin Paola Dolci Kalliopi Rantsiou | 2011 | Meat Science2011，，3： | 1 |
| 13 | Fermented Meat Products 显示文摘 | Kalliopi Rantsiou Luca Cocolin | 2008 | Molecular Techniques in the Microbial Ecology of Fermented Foods2008，，： | 1 |
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