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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Polymorphisms of DNA repair genes XRCC1 and XRCC3, interaction with environmental exposure and risk of chronic gastritis and gastric cancer显示文摘AIM: To evaluate the association between polymorphisms XRCC1 Arg194Trp and Arg399Gln and XRCC3Thr241Met and the risk for chronic gastritis and gastric cancer, in a Southeastern Brazilian population.METHODS: Genotyping by PCR-RFLP was carried out on 202 patients with chronic gastritis (CG) and 160 patients with gastric cancer (GC), matched to 202 (C1) and 150(C2) controls, respectively.RESULTS: No differences were observed among the studied grou ps with regard to the genotype distribution of XRCC1 codons 194 and 399 and of XRCC3 codon 241. However, the combined analyses of the three variant alleles (194Trp, 399Gln and 241Met) showed an increased risk for chronic gastritis when compared to the GC group. Moreover, an interaction between the polymorphic alleles and demographic and environmental factors was observed in the CG and GC groups. XRCC1 194Trp was associated with smoking in the CG group,while the variant alleles XRCC1 399Gln and XRCC3241Met were related with gender, smoking, drinking and H pylori infection in the CG and GC groups.CONCLUSION: Our results showed no evidence of a rela-tionship between the polymorphisms XRCC1Arg194Trp and Arg399Gln and XRCC3 Thr241Met and the risk of chronic gastritis and gastric cancer in the Brazilian population, but the combined effect of these variants may interact to increase the risk for chronic gastritis,considered a premalignant lesion. Our data also indicate a gene-environment interaction in the susceptibility to chronic gastritis and gastric cancer. | Márcia Cristina Duarte Jucimara Colombo Andrea Regina Baptista Rossit Alaor Caetano Aldenis Albaneze Borim Durval Wornrath Ana Elizabete Silva | 2005 | World Journal of Gastroenterology2005，11，42： | 11 |
| 3 | GSTT1,GSTM1 and CYP2E1 genetic polymorphisms in gastric cancer and chronic gastritis in a Brazilian population显示文摘MIM:To test the hypothesis that,in the Southeastern Brazilian population,the GSTT1,GSTM1 and CYP2E1 polymorphisms and putative risk factors are associated with an increased risk for gastric cancer.METHODS:We conducted a study on 100 cases of gastric cancer(GC),100 cases of chronic gastritis(CG),and 150 controls(C).Deletion of the GSTT1 and GSTM1 genes was assessed by multiplex PCR.CYP2E1/PsА genotyping was performed using a PCR-RFLP assay.RESULTS:No relationship between GSTT1/GSTM1 deletion and the c1/c2 genotype of CYP2E1 was observed among the three groups.However,a significant difference between CG and C was observde,due to a greater number of GSTT1/GSTM1 positive genotypes in the CG group.The GSTT1 null genotype occurred more frequently in Negroid subjicts,and the GSTM1 null genotype was observed mainly in individuals with chronic gastritis infected with H pylori.CONCLUSION:Our findings indecate that there is no obvious relationship between the GSTT1,GSTM1 and CYP2E1 polymorphisms and gastric cancer. | Jucimara Colombo Ana Elizabete Silva Andréa Regina Baptista Rossit Alaor Caetano Aldenis Albaneze Borim Durval Wohnrath | 2004 | World Journal of Gastroenterology2004，10，9： | 11 |
| 4 | Computational design in architecture:Defining parametric,generative,and algorithmic design显示文摘Computation-based approaches in design have emerged in the last decades and rapidly became popular among architects and other designers.Design professionals and researchers adopted different terminologies to address these approaches.However,some terms are used ambiguously and inconsistently,and different terms are commonly used to express the same concept.This paper discusses computational design(CD)and proposes an improved and sound taxonomy for a set of key CD terms,namely,parametric,generative,and algorithmic design,based on an extensive literature review from which different definitions by various authors were collected,analyzed,and compared. | Ines Caetano Luis Santos Antonio Leitao | 2020 | Frontiers of Architectural Research2020，9，2： | 6 |
| 5 | Differences in viral kinetics between genotypes 1 and 3 of hepatitis C virus and between cirrhotic and non-cirrhotic patients during antiviral therapy显示文摘AIM: To evaluate the impact of hepatitis C virus (HCV) infection with genotype 1 or 3 and the presence or absence of liver cirrhosis (LC) in the early viral kinetics response to treatment. METHODS: Naive patients (n = 46) treated with interferon-α (IFN-α) and ribavirin and followed up with frequent early HCV-RNA determinations were analysed. Patients were infected with genotype 1 (n = 28, 7 with LC) or 3 (n = 18, 5 with LC). RESULTS: The fi rst phase decline was larger in geno- type 3 patients than in genotype 1 patients (1.72 vs 0.95 log IU/mL, P < 0.001). The second phase slope decline was also larger in genotype 3 patients than in genotype 1 patients (0.87 vs 0.15 log/wk, P < 0.001). Differences were found in both cirrhotic and non-cirrhotic patients. Genotype 1 cirrhotic patients had a slower 2nd phase slope than non-cirrhotic patients (0.06 vs 0.18 log/wk, P < 0.02). None of genotype 1 cirrhotic patients had a 1st phase decline larger than 1 log (non-cirrhotic patients: 55%, P < 0.02). A similar trend toward a slower 2nd phase slope was observed in genotype 3 cirrhotic pa- tients but the 1st phase slope decline was not different. Sustained viral response was higher in genotype 3 pa- tients than in genotype 1 patients (72% vs 14%, P < 0.001) and in genotype 1 non-cirrhotic patients than in genotype 1 cirrhotic patients (19% vs 0%). A secondphase decline slower than 0.3 log/wk was predictive of non-response in all groups. CONCLUSION: Genotype 3 has faster early viral decline than genotype 1. Cirrhosis correlates with a slower 2nd phase decline and possibly with a lower 1st phase slope decline in genotype 1 patients. | José Eymard Medeiros-Filho Isabel Maria Vicente Guedes de Carvalho Mello Joo Renato Rebello Pinho Avidan U Neumann Fernanda de Mello Malta Luiz Caetano da Silva Flair José Carrilho | 2006 | World Journal of Gastroenterology2006，12，45： | 3 |
| 6 | Microalgae for biodiesel production and other applications: A review显示文摘 | Teresa M. Mata António A. Martins Nidia. S. Caetano | 2009 | Renewable and Sustainable Energy Reviews2009，，1： | 2 |
| 7 | Medial temporal lobe abnormalities in pediatric unipolar depression显示文摘 | Sheila C. Caetano Manoela Fonseca John P. Hatch Rene L. Olvera Mark Nicoletti Kristina Hunter Beny Lafer Steven R. Pliszka Jair C. Soares | 2007 | Neuroscience Letters2007，，3： | 2 |
| 8 | CD 64 distinguishes macrophages from dendritic cells in the gut and reveals the T h1‐inducing role of mesenteric lymph node macrophages during colitis显示文摘 | Samira Tamoutounour Sandrine Henri Hugues Lelouard Béatrice de Bovis Colin de Haar C. Janneke van der Woude Andrea M. Woltman Yasmin Reyal Dominique Bonnet Dorine Sichien Calum C. Bain Allan McI. Mowat Caetano Reis e Sousa Lionel F. Poulin Bernard Malisse | 2012 | Eur J Immunol2012，，12： | 2 |
| 9 | Neuropsychological performance and regional cerebral blood flow in obsessive-com- pulsive disorder显示文摘 | Lacerda AL Dalgalarrondo P Caetano D | 2003 | Prog Neuropsychopharmacol Biol Psychiatry2003，27，4： | 1 |
| 10 | Fast and sensitive silver staining of DNA in polyacryamid gels显示文摘 | Bassam B J Caetano A G Gresshoff P M | 1991 | Anal Biochem1991，196，1： | 1 |
| 11 | Effects of serum deprivation and cycloheximide on cell cycle of low and high passage porcine fetal fibroblasts 显示文摘 | Goissis MD Caetano HV Marques MG | 2007 | Reprod Domest Anim2007，42，6： | 1 |
| 12 | Fast and sensitive silver staining of DNA in polyacrylamide gels显示文摘 | BASSAM B J CAETANO G GRESSHOFF P M | 1991 | Analytical Biochemistry1991，196，1： | 1 |
| 13 | Chitosan-alginate membranes accelerate wound healing 显示文摘 | Caetano GF Cipriani MA Moretti TA | 2015 | J Biomed Mater Res B Appl Biomater2015，103，5： | 1 |
| 14 | Fast and sensitive silver staining of DNA in polyacryamid gels显示文摘 | Bassam B J Caetano A G Gresshoff P M | 1991 | Analytical Biochemistry1991，196，： | 1 |
| 15 | Formation of midchain alkane keto-ols by post-depositional oxidation of mid-chain diols in Mediterranean sapropels 显示文摘 | Ferreira A M Miranda A Caetano M | 2001 | Organic Geochemistry2001，32，2： | 1 |
| 16 | Silver Staining of DNA in Polyacrylamide Gels 显示文摘 | Brant J Bassam Gustavo Caetano Anolle | 1993 | Applied Biochemistry and Biotechnology1993，42，： | 1 |
| 17 | Second-order interference with orthogonally polarized pseudo-thermal beams显示文摘 | Vidal I Caetano D P | 2008 | Physical Review A2008，78，05： | 1 |
| 18 | Touch activates hu- man auditory cortex显示文摘 | Schurmann M Caetano G Hlushchuk Y | 2006 | Neuroitrmge2006，30，4： | 1 |
| 19 | The 5-year course of intimate partner violence among White,Black,and Hispanic couples in the United States显示文摘 | Caetano R Field CA Ramisetty-Mikler S | | 0，，9： | 1 |
| 20 | Lower N-acetyl-aspartate levels in prefrontal cortices in pediatric bipolar disorder:a(1)H magnetic resonance spectroscopy study显示文摘 | Caetano SC Olvera RL Hatch JP | | 0，，： | 1 |
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