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| 1 | Phenformin alone or combined with gefitinib inhibits bladder cancer via AMPK and EGFR pathways显示文摘Background:In previous studies,we have shown that the combination of metformin and gefitinib inhibits the growth of bladder cancer cells.Here we examined whether the metformin analogue phenformin,either used alone or in combination with gefitinib,could inhibit growth of bladder cancer cells.Methods:The growth-inhibitory effects of phenformin and gefitinib were tested in one murine and two human bladder cancer cell lines using MTT and clonogenic assays.Effects on cell migration were assessed in a wound healing assay.Synergistic action between the two drugs was assessed using CompuSyn software.The potential involvement of AMPK and EGFR pathways in the effects of phenformin and gefitinib was explored using Western blotting.Results:In MTT and clonogenic assays,phenformin was>10-fold more potent than metformin in inhibiting bladder cancer cell growth.Phenformin also potently inhibited cell migration in wound healing assays,and promoted apop-tosis.AMPK signaling was activated;EGFR signaling was inhibited.Phenformin was synergistic with gefitinib,with the combination of drugs showing much stronger anticancer activity and apoptotic activation than phenformin alone.Conclusions:Phenformin shows potential as an effective drug against bladder cancer,either alone or in combination with gefitinib. | Yanjun Huang Sichun Zhou Caimei He Jun Deng Ting Tao Qiongli Su Kwame Oteng Darko Mei Peng Xiaoping Yang | 2018 | Cancer Communications2018，38，1： | 5 |
| 2 | 苯乙双胍单药或联合吉非替尼通过AMPK和EGFR通路抑制膀胱癌显示文摘背景与目的在前期研究中,我们发现二甲双胍和吉非替尼联合使用可抑制膀胱癌细胞生长。本文旨在研究二甲双胍类似物——苯乙双胍单药或联合吉非替尼能否抑制膀胱癌细胞生长。方法在1种鼠源和2种人源膀胱癌细胞系中,采用MTT和克隆形成实验检测苯乙双胍和吉非替尼对细胞生长的抑制作用。采用伤口愈合实验检测细胞迁移情况。使用CompuSyn软件评估2种药物之间的协同作用。采用蛋白免疫印迹实验分析苯乙双胍和吉非替尼对AMPK和EGFR通路的潜在作用。结果 MTT和克隆形成实验结果显示,苯乙双胍抑制膀胱癌细胞生长的作用是二甲双胍的10倍以上。伤口愈合实验结果显示,苯乙双胍可有效抑制细胞迁移,并促进凋亡。AMPK信号被激活,EGFR信号被抑制。苯乙双胍与吉非替尼可协同作用,二者联合使用比苯乙双胍单药表现出更强的抑癌活性和促凋亡活性。结论苯乙双胍单药或联合吉非替尼均可能成为治疗膀胱癌的有效药物。 | Yanjun Huang Sichun Zhou Caimei He Jun Deng Ting Tao Qiongli Su Kwame Oteng Darko Mei Peng Xiaoping Yang | 2019 | 癌症2019，38，5： | 1 |
| 3 | Drug Susceptibility Test of Volatile Oil of Artemisiaargyi to Avian Pathogenic Escherichia coli显示文摘The volatile oil of Artemisia argyi was extracted by ultrasonic assisted extraction, and the extraction rate of volatile oil was 0.68%. The volatile oil of A. argyi was emulsified with 1% Tween-80, and drug susceptibility test was conducted with avian Escherichia coli. The results showed that the volatile oil of A. argyi had antibacterial effect against avian E. coli, and the minimal inhibitory concentration was 50 mg/mL. Taking six common antibiotics as the control, drug susceptibility test was conducted with volatile oil of A. argyi. The results showed that 10 strains of E. coli were sensitive to the volatile oil of A. argyi, three of which had different degrees of resistance and one had the tendency of resistance. | pan baiming he caimei liang changxiang zhou huiling li xue | 2018 | Animal Husbandry and Feed Science2018，10，4： | 1 |
| 4 | Graphene-Gold Nanoparticle-modified Electrochemical Sensor for Detection of Kanamycin Based on Target-induced Aptamer Displacement显示文摘 | ZHENG Jingyi FENG Rongrong HE Caimei LI Xiaoxia | 2018 | Chemical Research in Chinese Universities2018，34，6： | 0 |
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