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| 1 | Prospective randomized trial comparing hyperfractionated versus conventional radiotherapy in stages Ⅲ and Ⅳ oropharyngeal carcinoma显示文摘 | Pinto L H J Canary P C V Araugo C M M | 1991 | Int J Radiat Oncol BioI Phys1991，21，3： | 1 |
| 2 | Living with heart failure:Depression and quality of life in patients and spouses显示文摘 | Martensson J Dracup K Canary C | 2003 | Heart Lung Transplant2003，22，： | 1 |
| 3 | Living with heart failure: Depression and quality of life in patients and spou ses显示文摘 | Martensson J Dracup K Canary C | 2003 | J Heart Lung Transplant2003，22，4： | 1 |
| 4 | laving with heart failure:depression and quality of life in patients and spouses 显示文摘 | Martensson J Dracup K Canary C | 2003 | J Heart Lung Transplant2003，22，4： | 1 |
| 5 | The Colorado thyrod disease prevalence study 显示文摘 | Canaris GJ Manowitz NR Mayor C | 2000 | Arch Intern Med2000，160，： | 1 |
| 6 | Living with heart fail- ure:Depression and quality of life in patients and spouses显示文摘 | Martensson J Dracup K Canary C | 2003 | J Heart Lung Transpl2003，22，4： | 1 |
| 7 | Bone marrow progenitor cells do not contribute to liver fibrogenic cells显示文摘AIM:To investigate the contribution of bone marrow(BM) cells to hepatic fibrosis.METHODS:To establish a model of chimerism,C57Bl/6 female mice were subjected to full-body irradiation(7 Gy) resulting in BM myeloablation.BM mononuclear cells obtained from male transgenic mice expressing enhanced green fluorescent protein(GFP) were used for reconstitution.Engraftment was confirmed by flow cytometry.To induce liver injury,chimeric animals received carbon tetrachloride(CCl4) 0.5 mL/kg intraperitoneally twice a week for 30 d(CCl4 30 d) and age-matched controls received saline(Saline 30 d).At the end of this period,animals were sacrificed for post mortem analysis.Liver samples were stained with hematoxylin and eosin to observe liver architectural changes and with Sirius red for collagen quantification by morphometric analysis.α-smooth muscle actin(α-SMA) was analyzed by confocal microscopy to identify GFP+ cells with myofibroblast(MF) characteristics.Liver tissue,BM and peripheral blood were collected and prepared for flow cytometric analysis using specific markers for detection of hepatic stellate cells(HSCs) and precursors from the BM.RESULTS:Injury to the liver induced changes in the hepatic parenchymal architecture,as reflected by the presence of inflammatory infiltrate and an increase in collagen deposition(Saline 30 d = 11.10% ± 1.12% vs CCl4 30 d = 12.60% ± 0.73%,P = 0.0329).Confocal microscopy revealed increased reactivity against α-SMA in CCl4 30 d compared to Saline 30 d,but there was no co-localization with GFP+ cells,suggesting that cells from BM do not differentiate to MFs.Liver flow cytometric analysis showed a significant increase of CD45+/GFP+ cells in liver tissue(Saline 30 d = 3.2% ± 2.2% vs CCl4 30 d = 5.8% ± 1.3%,P = 0.0458),suggesting that this increase was due to inflammatory cell infiltration(neutrophils and monocytes).There was also a significant increase of common myeloid progenitor cells(CD117+/CD45+) in the livers of CCl4-treated animals(Saline 30 d = 2.16% ± 1.80% vs CCl4 30 d = 5.60% ± 1.30%,P = 0.0142).In addition the GFP-/CD38+/CD45-subpopulation was significantly increased in the CCl4 30 d group compared to the Saline 30 d group(17.5% ± 3.9% vs 9.3% ± 2.4%,P = 0.004),indicating that the increase in the activated HSC subpopulation was not of BM origin.CONCLUSION:BM progenitor cells do not contribute to fibrosis,but there is a high recruitment of inflammatory cells that stimulates HSCs and MFs of liver origin.? 2012 Baishideng.All rights reserved. | Bruno Diaz Paredes Lanuza Alaby Pinheiro Faccioli Luiz Fernando Quintanilha Karina Dutra Asensi Camila Zaverucha do Valle Paulo César Canary Christina Maeda Takiya Antonio Carlos Campos de Carvalho Regina Coeli dos Santos Goldenberg | 2012 | World Journal of Hepatology2012，4，10： | 0 |
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