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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | Diagnosis of bile duct hepatocellular carcinoma thrombus without obvious intrahepatic mass显示文摘AIM:To study the diagnosis of hepatocellular carcinoma(HCC)presenting as bile duct tumor thrombus with no detectable intrahepatic mass.METHODS:Six patients with pathologically proven bile duct HCC thrombi but no intrahepatic mass demonstrated on the preoperative imaging or palpated intrahepatic mass during operative exploration,were collected.Their clinical and imaging data were retrospectively analyzed.The major findings or signs on comprehensive imaging were correlated with the surgical and pathologic findings.RESULTS:Jaundice was the major clinical symptom of the patients.The elevated serum total bilirubin,direct bilirubin and alanine aminotransferase levels were in concordance with obstructive jaundice and the underlying liver disease.Of the 6 patients showing evidence of viral hepatitis,5 were positive for serum alpha fetoprotein and carbohydrate antigen 19-9,and 1 was positive for serum carcinoembryonic antigen.No patient was correctly diagnosed by ultrasound.The main features of patients on comprehensive imaging were filling defects with cup-shaped ends of the bile duct,with large filling defects presenting as casting moulds in the expanded bile duct,hypervascular intraluminal nodules,debris or blood clots in the bile duct.No obvious circular thickening of the bile duct walls was observed.CONCLUSION:Even with no detectable intrahepatic tumor,bile duct HCC thrombus should be considered in patients predisposed to HCC,and some imaging signs are indicative of its diagnosis. | Long XY Li YX Wu W Li L Cao J. | 2010 | World Journal of Gastroenterology2010，16，39： | 11 |
| 3 | 脂蛋白a水平与冠状动脉粥样硬化性心脏病患者复发事件的关系显示文摘脂蛋白a是否是冠状动脉粥样硬化性心脏病(冠心病)患者复发性心血管事件(recurrent cardiovascular events,RCVEs)的预测因子尚未确定。本研究旨在探讨脂蛋白a对一个真实世界、大规模首诊心血管事件(cardiovascular event,CVE)患者队列中RCVEs的潜在影响。方法:本实验为多中心的前瞻性研究,纳入经血管造影诊断的冠心病并经历首次CVE的患者7562例。入院时测量所有患者的脂蛋白a浓度. | 周卫(译) 叶鹏(校) Liu HH Cao YX Jin JL Zhang HW Hua Q Li YF Guo YL Zhu CG Wu NQ Gao Y Xu RX Hong LF | 2020 | 中华高血压杂志2020，28，5： | 5 |
| 4 | Step Sintering of Microwave Heating and MicrowavePlasma Heating for Alumina Ceramics显示文摘The invention of microwave induced plasma during microwave heating makes it possible to confine plasma inside a crucible, in the pressure range of 1.33 Pa to 0.5 MPa, and above 900℃.In this study, the step sintering of microwave heating and microwave plasma heating (SSMP)was proposed. In this process, microwave heating and plasma heating was ingeniously combined in one chamber, and vacuum was unnecessary for microwave induced plasma. In the first step, the microwave energy directly heated alumina ceramics to a given temperature. above which microwave will mainly ionize the gas and sustain a plasma. Then the plasma further heated alumina ceramics to the final temperature to finish sintering. Maintaining the advantages of microwave heating and plasma heating, this step sintering approach will overcome the limitation of both processes for processing ceramics. and it will be suitable for sintering all kinds of ceramics in | Zhang, JS Cao, L Yang, YJ Diao, YX Shen, XX | 1999 | Journal of Materials Science & Technology1999，15，5： | 2 |
| 5 | Enhanced binding of Sp1/Sp3 transcription factors mediates the hyperoxia-induced increased expression of the lung typeⅠ cell gene T1alpha显示文摘 | Cao YX Ramirez MI Williams MC | 2003 | J Cell Biochem2003，89，5： | 1 |
| 6 | Artificial oocyte activation and human failed-matured oocyte vitrification followed by in vitro maturation 显示文摘 | Liu Y Cao YX Zhang ZG | 2013 | Zygote2013，21，1： | 1 |
| 7 | Comparison of survival and embryonic development inhuman ooCytes cryopreserved by slow- freezing and vitrification显示文摘 | Cao YX Xing Q Li L | 2009 | Fertil Steril2009，92，： | 1 |
| 8 | Hydrogen sulphide is an inhibitor of L-type calcium channels and mechanical contraction in rat cardiomyocytes 显示文摘 | Sun YG Cao YX Wang WW | 2008 | Cardiovasc Res2008，79，4： | 1 |
| 9 | Vascular endothelin ET (B) recep-tor-mediated contraction requires phosphorylation of ERK1/2 proteins显示文摘 | Luo G Jamali R Cao YX | 2006 | Eur J Pharmacol2006，538，： | 1 |
| 10 | Fertility preservation with immature and in vitro matured oocytes 显示文摘 | Cao YX Chian RC | 2009 | Sernin Reprod Med2009，27，6： | 1 |
| 11 | Transcriptional up-regulation in expression of 5-hydroxytryptamine2A and transcriptional down-regulation of an-giotensin Ⅱ type 1 receptors during organ culture of rat mesenteric artery显示文摘 | Luo G Xu CB Cao YX | 2004 | Basic Clin Phannacol Toxicol2004，95，： | 1 |
| 12 | Polymorphisms of the peroxisome proliferator-activate receptor-γ and its coactivator-1α genes in Chinese women with polycystic ovary syndrome显示文摘 | Wang Y Wu XK Cao YX | 2006 | Fertil Steril2006，85，5： | 1 |
| 13 | Effects of microinjection of melatonin and its receptor antagonists into anterior hypotha- lamic area on blood pressure and heart rate in rats显示文摘 | Ding CN Cao YX Zhou L | 2001 | ActaPharmacol Sin2001，22，11： | 1 |
| 14 | GnRH I and II up-regulate MMP-26 expression through the JNK pathway in human cytotrophoblasts 显示文摘 | Liu J Cao B Li YX | 2010 | Reprod Biol Endocrinol2010，8，1： | 1 |
| 15 | Effects of electroacupuncture on pressor response to angiotensin-(1-7) by amino acid release in the rostral ventrolateral medulla显示文摘 | Wang J Shen LL Cao YX Zhu DN | 2003 | Acupunct Electrother Res2003，28，： | 1 |
| 16 | Comparison of survival and embryonic development in human oocytes cryopreserved by slow-freezing and vitrification显示文摘 | Cao YX Xing Q Li L | 2009 | Fertil Steril2009，92，4： | 1 |
| 17 | Comparison of survival and embryonic development in human oocytes cryopreserved by slowfreezing and vitrification 显示文摘 | Cao YX Xing Q Li L | 2009 | F ertil Steri!2009，92，4： | 1 |
| 18 | Enhanced binding of Sp1/Sp3 transcription factors mediates the hyperoxia-induced increased expression of the lung type I cell gene T1显示文摘 | Cao YX Ramirez MI Williams MC | 2003 | J Cell Biochem2003，89，5： | 1 |
| 19 | Fertility preservation with immature and in vitro matured oocytes显示文摘 | Cao YX Chian RC | 2009 | Semin Reprod Med2009，27，6： | 1 |
| 20 | Altered endothelin receptor expression and affinity in spontaneously hypertensive rat cerebral and coronary arteries显示文摘 | CAO L CAO YX XU CB | 2013 | PLoS One2013，8，73： | 1 |
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