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| 1 | Promotion of the articular cartilage proteoglycan degradation by T-2 toxin and selenium protective effect显示文摘Objective: To identify the relationship between T-2 toxin and Kashin-Beck disease (KBD),the effects of T-2 toxin on aggrecan metabolism in human chondrocytes and cartilage were investigated in vitro. Methods: Chondrocytes were isolated from human articular cartilage and cultured in vitro. Hyaluronic acid (HA),soluble CD44 (sCD44),IL-1β and TNF-α levels in super-natants were measured by enzyme-linked immunosorbent assay (ELISA). CD44 content in chondrocyte membrane was deter-mined by flow cytometry (FCM). CD44,hyaluronic acid synthetase-2 (HAS-2) and aggrecanases mRNA levels in chondrocytes were determined using reverse transcription polymerase chain reaction (RT-PCR). Immunocytochemical method was used to investigate expressions of BC-13,3-B-3(-) and 2-B-6 epitopes in the cartilage reconstructed in vitro. Results: T-2 toxin inhibited CD44,HAS-2,and aggrecan mRNA expressions,but promoted aggrecanase-2 mRNA expression. Meanwhile,CD44 expression was found to be the lowest in the chondrocytes cultured with T-2 toxin and the highest in control plus selenium group. In addition,ELISA results indicated that there were higher sCD44,IL-1β and TNF-α levels in T-2 toxin group. Similarly,higher HA levels were also observed in T-2 toxin group using radioimmunoprecipitation assay (RIPA). Furthermore,using monoclonal antibodies BC-13,3-B-3 and 2-B-6,strong positive immunostaining was found in the reconstructed cartilage cultured with T-2 toxin,whereas no positive staining or very weak staining was observed in the cartilage cultured without T-2 toxin. Selenium could partly inhibit the effects of T-2 toxin above. Conclusion: T-2 toxin could inhibit aggrecan synthesis,promote aggrecanases and pro-inflammatory cytokines production,and consequently induce aggrecan degradation in chondrocytes. These will perturb metabolism balance between aggrecan synthesis and degradation in cartilage,inducing aggrecan loss in the end,which may be the initiation of the cartilage degradation. | Si-yuan LI Jun-ling CAO Zhong-li SHI Jing-hong CHEN Zeng-tie ZHANG Clare E. HUGHES Bruce CATERSON | 2008 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2008，9，1： | 16 |
| 2 | Increased expression of chondroitin sulphate proteoglycans in rat hepatocellular carcinoma tissues显示文摘AIM:To investigate the expression of chondroitin sulphate proteoglycans(CSPGs)in rat liver tissues of hepatocellular carcinoma(HCC).METHODS:Thirty male Sprague Dawley rats were randomly divided into two groups:control group(n=10) and HCC model group(n=20).Rats in the HCC model groups were intragastrically administrated with 0.2%(w/v)N-diethylnitrosamine(DEN)every 5 d for 16 wk,whereas 0.9%(w/v)normal saline was administered to rats in the control group.After 16 wk from the initiation of experiment,all rats were killed and livers were collected and fixed in 4%(w/v)paraformaldehyde.All tissues were embedded in paraffin and sectioned.Histological staining(hematoxylin and eosin and Toluidine blue)was performed to demonstrate the onset of HCC and the content of sulphated glycosaminoglycan(sGAG).Immunohistochemical staining was performed to investigate the expression of chondroitin sulphate(CS)/dermatan sulphate(DS)-GAG,heparan sulphate(HS)-GAG,keratan sulphate(KS)-GAG in liver tissues.Furthermore,expression and distribution of CSPG family members,including aggrecan,versican,biglycan and decorin in liver tissues,were also immunohistochemically determined.RESULTS:After 16 wk administration of DEN,malignant nodules were observed on the surface of livers from the HCC model group,and their hepatic lobule structures appeared largely disrupted under microscope.Toluidine blue staining demonstrated that there was an significant increase in sGAG content in HCC tissues when compared with that in the normal liver tissues from the control group[0.37±0.05 integrated optical density per stained area(IOD/area)and 0.21± 0.01 IOD/area,P<0.05].Immunohistochemical studies demonstrated that this increased sGAG in HCC tissues was induced by an elevated expression of CS/DS(0.28±0.02 IOD/area and 0.18±0.02 IOD/area,P< 0.05)and HS(0.30±0.03 IOD/area and 0.17±0.02 IOD/area,P<0.01)but not KS GAGs in HCC tissues.Further studies thereby were performed to investigate the expression and distribution of several CSPG components in HCC tissues,including aggrecan,versican,biglycan and decorin.Interestingly,there was a distinct distribution pattern for these CSPG components between HCC tissues and the normal tissues.Positive staining of aggrecan,biglycan and decorin was localized in hepatic membrane and/or pericellular matrix in normal liver tissues;however,their expression was mainly observed in the cytoplasm,cell membranes in hepatoma cells and/or pericellular matrix within HCC tissues.Semi-quantitative analysis indicated that there was a higher level of expression of aggrecan(0.43± 0.01 and 0.35±0.03,P<0.05),biglycan(0.32±0.01 and 0.25±0.01,P<0.001)and decorin(0.29±0.01 and 0.26±0.01,P<0.05)in HCC tissues compared with that in the normal liver tissues.Very weak versican positive staining was observed in hepatocytes near central vein in normal liver tissues;however there was an intensive versican distribution in fibrosis septa between the hepatoma nodules.Semi-quantitative analysis indicated that the positive rate of versican in hepatoma tissues from the HCC model group was much higher than that in the control group(33.61%and 21.28%,P <0.05).There was no positive staining in lumican and keratocan,two major KSPGs,in either normal or HCC liver tissues.CONCLUSION:CSPGs play important roles in the onset and progression of HCC,and may provide potential therapeutic targets and clinical biomarkers for this prevalent tumor in humans. | Xiao-Li Jia Si-Yuan Li Shuang-Suo Dang Yan-An Cheng Xin Zhang Wen-Jun Wang Clare E Hughes Bruce Caterson | 2012 | World Journal of Gastroenterology2012，18，30： | 2 |
| 3 | Isolated Low Levels of High-Density Lipoprotein Cholesterol Are Associated With an Increased Risk of Coronary Heart Disease: An Individual Participant Data Meta-Analysis of 23 Studies in the Asia-Pacific Region显示文摘 | Rachel R. Huxley Federica Barzi Tai Hing Lam Sebastien Czernichow Xianghua Fang Tim Welborn Jonathan Shaw Hirotsugu Ueshima Paul Zimmet Sun Ha Jee Jeetesh V. Patel Ian Caterson Vlado Perkovic Mark Woodward | 2011 | Circulation2011，，19： | 2 |
| 4 | Ischemia-reperfusion injury in vascularized composite allotransplantation显示文摘 | Caterson EJ Lopez J Medina M | 2013 | J Craniofac Surg2013，24，： | 1 |
| 5 | Effect of sibutramine on cardiovascular outcomes in overweight and obese subjects显示文摘 | James WP Caterson ID Coutinho W | 2010 | N EngI J Med2010，363，10： | 1 |
| 6 | Associat- ion of serum leptin with hypoventilation in human obesity 显示文摘 | PHIPPS P R STARRITY E CATERSON I | 2002 | Thorax2002，57，1： | 1 |
| 7 | Matrix metalloproteinases and aggrecanase:their role in disorders of the human intervertebral disc显示文摘 | Roberts S Caterson B Menage J | 2000 | Spine2000，25，23： | 1 |
| 8 | Electrospun nanofi- brous structure a novel scaffold for tissue engineering显示文摘 | Li W J Laurencin CT Caterson | 2002 | J Biomed Mater Res2002，60，： | 1 |
| 9 | Human inter- vertebral disc aggrecan inhibits endothelial cell adhesion and cell migration in vitro 显示文摘 | Johnson WE Caterson B Eisenstein SM | 2005 | Spine2005，30，10： | 1 |
| 10 | Freeing the bain from the perineuronal net显示文摘 | Fox K Caterson B | 2002 | Science2002，228，5596： | 1 |
| 11 | Matrix metalloproteinases and aggrecanase:their role in disorders of the human intervertebral disc显示文摘 | Roberts S Caterson B Menage J | 2000 | Spine2000，25，23： | 1 |
| 12 | Electrospun nanofibers stmcture:A novel seaffold for tissue engineering显示文摘 | Li W J Laurencin C T Caterson E J | 2002 | Journal of Biomedical Materials Research2002，60，4： | 1 |
| 13 | Matrix metalloproteinases and aggre canase:their role in disorders of the human intervertebral disc显示文摘 | Roberts S Caterson B Menage J | 2000 | Spine2000，25，23： | 1 |
| 14 | Mechanical determinants of osteoarthrosis 显示文摘 | RADIN E L BURR D B CATERSON B | 1991 | Semin Arthritis Rheum1991，21，32： | 1 |
| 15 | Clinical impact upon wound healing and inflammation in moist, wet, and dry environments显示文摘 | Junker JP Kamel RA Caterson E J | 2013 | Adv Wound Care2013，2，7： | 1 |
| 16 | Eleetrospun nanofibrous structure: a novel scaffold for tissue engineering 显示文摘 | Li W J Laurencin C T Caterson E J | 2002 | Journal of biomedical materials research2002，60，4： | 1 |
| 17 | Electrospun nanofibrous structure: a novel scaffold for tissue englneering显示文摘 | Li WJ Laurencin CT Caterson EJ | 2002 | J Biomed Mater Res2002，60，4： | 1 |
| 18 | Matrix metallopro- teinases and aggrecanase: their role in disorders of the human intervertebral disc显示文摘 | Roberts S Caterson B Menage J | 2000 | Spine~2000，25，23： | 1 |
| 19 | Human intervertebral disc aggrecan inhibits nerve growth in vitro显示文摘 | Caterson B Eisenstein SM | 2002 | Arthritis Rheum2002，46，10： | 1 |
| 20 | Matrix metalloproteinases and aggrencanase: their role in disorders or flumar intervertebral disc 显示文摘 | Roberts S Caterson B Menage J | 2000 | Spine2000，25，： | 1 |
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