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3篇 您的检索式:作者名="Chai Xianfeng"
    题名 作者 年代 出处 被引量
1Excellent Ag se- lective receptors:Syntheses and eomplexation properties of no- vel biscalixarene with benzalazine groups显示文摘Yang Fafu Hong Biqiong Chai Xianfeng 2009Supramoleeu lar Chemistry2009,21,8:1
2Fine-tuning brassinosteroid biosynthesis via 3′UTR-dependent decay of CPD mRNA modulates wood formation in Populus显示文摘Brassinosteroids(BRs)are plant hormones that regulate wood formation in trees.Currently,little is known about the post-transcriptional regulation of BR synthesis.Here,we show that during wood formation,fine-tuning BR synthesis requires 3′UTR-dependent decay of Populus CONSTITUTIVE PHOTOMORPHOGENIC DWARF 1(PdCPD1).Overexpression of PdCPD1 or its 3′UTR fragment resulted in a significant increase of BR levels and inhibited secondary growth.In contrast,transgenic poplars repressing PdCPD13′UTR expression displayed moderate levels of BR and promoted wood formation.We show that the Populus GLYCINE-RICH RNA-BINDING PROTEIN1(PdGRP1)directly binds to a GU-rich element in 3′UTR of Pd CPD1,leading to its mRNA decay.We thus provide a post-transcriptional mechanism underlying BRs synthesis during wood formation,which may be useful for genetic manipulation of wood biomass in trees.Dian Wang Xiaoning Hao Li Xu Mengyan Zhao Congpeng Wang Xihao Yu Yingzhen Kong Mengzhu Lu Gongke Zhou Guohua Chai Xianfeng Tang 2023Journal of Integrative Plant Biology2023,65,8:0
3Wood forming tissue-specific expression of PdSuSy and HCHL increases holocellulose content and improves saccharification in Populus显示文摘Development of strategies to deconstruct lignocellulosic biomass in tree species is essential for biofuels and biomaterials production.We applied a wood forming tissue-specific system in a hybrid poplar to express both PdSuSy(a sucrose synthase gene from Populus deltoides×P.euramericana that has not been functionally characterized)and HCHL(the hydroxycinnamoyl-CoA hydratase-lyase gene from Pseudomonas fluorescens,which inhibits lignin polymerization in Arabidopsis).The PdSuSy-HCHL overexpression poplars correspondingly driven by the promoters of Arabidopsis AtCesA7 and AtC4 H resulted in a significant increase in cellulose(>8%),xylan(>12%)and glucose(>29%)content,accompanying a reduction in galacturonic acid(>36%)content,compared to control plants.The saccharification efficiency of these overexpression poplars was dramatically increased by up to 27%,but total lignin content was unaffected.These transgenic poplars showed inhibited growth characteristics,including>16%reduced plant height,>10% reduced number of internodes,and>18% reduced fresh weight after growth of 4 months,possibly due to relatively low expression of HCHL in secondary xylem.Our results demonstrate the structural complexity and interaction of the cell wall polymers in wood tissue and outline a potential method to increase biomass saccharification in woody species.Yang Zhang Hua Xu Yingzhen Kong Jiawen Hua Xianfeng Tang Yamei Zhuang Yue Bai Gongke Zhou Guohua Chai 2021Journal of Forestry Research2021,32,4:0
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