6篇
您的检索式：作者名="Chang Wonseok"
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| 1 | Expression and Role of Voltage-Gated Sodium Channels in Human Dorsal Root Ganglion Neurons with Special Focus on Nav1.7,Species Differences, and Regulation by Paclitaxel显示文摘Voltage-gated sodium channels(Navs) play an important role in human pain sensation. However, the expression and role of Nav subtypes in native human sensory neurons are unclear. To address this issue, we obtained human dorsal root ganglion(hDRG) tissues from healthy donors. PCR analysis of seven DRG-expressed Nav subtypes revealed that the hDRG has higher expression of Nav1.7(~50% of total Nav expression) and lower expression of Nav1.8(~12%), whereas the mouse DRG has higher expression of Nav1.8(~45%) and lower expression of Nav1.7(~18%). To mimic Nav regulation in chronic pain, we treated hDRG neurons in primary cultures with paclitaxel(0.1-1 μmol/L) for 24 h. Paclitaxel increased the Nav 1.7 but not Nav1.8 expression and also increased the transient Na^+ currents and action potential firing frequency in small-diameter(<50 μm) hDRG neurons. Thus, the hDRG provides a translational model in which to study'human pain in a dish' and test new pain therapeutics. | Wonseok Chang Temugin Berta Yong Ho Kim Sanghoon Lee Seok-Yong Lee Ru-Rong Ji | 2018 | Neuroscience Bulletin2018，34，1： | 12 |
| 2 | Differential Inhibition of Nav1.7 and Neuropathic Pain by Hybridoma-Produced and Recombinant Monoclonal Antibodies that Target Nav1.7显示文摘The voltage-gated Na^+ channel subtype Nav1.7 is important for pain and itch in rodents and humans. We previously showed that a Nav1.7-targeting monoclonal antibody(SVmab) reduces Na+ currents and pain and itch responses in mice. Here, we investigated whether recombinant SVmab(rSVmab) binds to and blocks Nav1.7 similar to SVmab. ELISA tests revealed that SVmab was capable of binding to Nav1.7-expressing HEK293 cells,mouse DRG neurons, human nerve tissue, and the voltagesensor domain Ⅱ of Nav1.7. In contrast, rSVmab showed no or weak binding to Nav1.7 in these tests. Patch-clamp recordings showed that SVmab, but not rSVmab, markedly inhibited Na+ currents in Nav1.7-expressing HEK293 cells. Notably, electrical field stimulation increased the blocking activity of SVmab and rSVmab in Nav1.7-expressing HEK293 cells. SVmab was more effective than rSVmab in inhibiting paclitaxel-induced mechanical allodynia. SVmab also bound to human DRG neurons and inhibited their Na^+ currents. Finally, potential reasons for the differential efficacy of SVmab and rSVmab and future directions are discussed. | Sangsu Bang Jiho Yoo Xingrui Gong Di Liu Qingjian Han Xin Luo Wonseok Chang Gang Chen Sang-Taek Im Yong Ho Kim Judith A.Strong Ma-Zhong Zhang Jun-Ming Zhang Seok-Yong Lee Ru-Rong Ji | 2018 | Neuroscience Bulletin2018，34，1： | 2 |
| 3 | Decolorizing dye wastewater with low temperature catalytic oxidation显示文摘 | Daewon Pak Wonseok Chang | 1999 | Water Science and Tehchnology1999，40，45： | 1 |
| 4 | Decolorizing dye wastewater with low temperature catalytic oxidation显示文摘 | Pak Daewon Chang Wonseok | 1999 | Water Science and Technology1999，40，45： | 1 |
| 5 | Effect of zeolite media for the treatment of textile wastewater in abiological aerated filter显示文摘 | Chang Wonseok Hong Seokwon Park Joonkyu | 2002 | Process Biochemistry2002，37，： | 1 |
| 6 | Ammonium nitrogen removal characteristics of zeolite media in a Biological Aerated Filter (BAF) for the treatment of textile wastewater显示文摘 | WonSeok Chang HungThuan Tran Doo-Hyun Parki | 2009 | Joumal of Industrial and Engineering Chemistry2009，41，15： | 1 |
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