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| 1 | HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation显示文摘Light is arguably one of the most important environmental factors that determines virtually all aspects of plant growth and development,but the molecular link between light signaling and the autophagy pathway has not been elucidated in plants.In this study,we demonstrate that autophagy is activated during light-to-dark conversion though transcriptional upregulation of autophagy-related genes(ATGs).We showed that depletion of the ELONGATED HYPOCOTYL 5(HY5),a key component of light signaling,leads to enhanced autophagy activity and resistance to extended darkness and nitrogen starvation treatments,contributing to higher expression oiATGs.HY5 interacts with and recruits HISTONE DEACETYLASE 9(HDA9)to ATG5 and ATG8e loci to repress their expression by deacetylation of the Lys9 and Lys27 of histone 3.Furthermore,we found that both darkness and nitrogen depletion induce the degradation of HY5 via 26S proteasome and the concomitant disassociation of HDA9 from ATG5 and ATG8e loci,leading to their depression and thereby activated autophagy.Genetic analysis further confirmed that HY5 and HDA9 act synergistically and function upstream of the autophagy pathway.Collectively,our study unveils a previously unknown transcriptional and epigenetic network that regulates autophagy in response to light-to-dark conversion and nitrogen starvation in plants. | Chao Yang Wenjin Shen Lianming Yang Yun Sun Xibao Li Minyi Lai Juan Wei Chaojun Wang Yingchao Xu Faqiang Li Shan Liang Chengwei Yang Shangwei Zhong Ming Luo Caiji Gao | 2020 | Molecular Plant2020，13，3： | 8 |
| 2 | LncRNA DPP10-AS1 promotes malignant processes through epigenetically activating its cognate gene DPP10 and predicts poor prognosis in lung cancer patients显示文摘Objective:The purpose of this study was to explore the function and gene expression regulation of the newly identified lnc RNA DPP10-AS1 in lung cancer,and its potential value as a prognostic biomarker.Methods:q RT-PCR and Western blot were conducted to detect the expression of DDP10-AS1 and DPP10 in lung cancer cell lines and tissues.The effects of DDP10-AS1 on DPP10 expression,cell growth,invasion,apoptosis,and in vivo tumor growth were investigated in lung cancer cells by Western blot,rescue experiments,colony formation,flow cytometry,and xenograft animal experiments.Results:The novel antisense lnc RNA DPP10-AS1 was found to be highly expressed in cancer tissues(P<0.0001),and its upregulation predicted poor prognosis in patients with lung cancer(P=0.0025).Notably,DPP10-AS1 promoted lung cancer cell growth,colony formation,and cell cycle progression,and repressed apoptosis in lung cancer cells by upregulating DPP10 expression.Additionally,DPP10-AS1 facilitated lung tumor growth via upregulation of DPP10 protein in a xenograft mouse model.Importantly,DPP10-AS1 positively regulated DPP10 gene expression,and both were coordinately upregulated in lung cancer tissues.Mechanically,DPP10-AS1 was found to associate with DPP10 m RNA but did not enhance DPP10 m RNA stability.Hypomethylation of DPP10-AS1 and DPP10 contributed to their coordinate upregulation in lung cancer.Conclusions:These findings indicated that the upregulation of the antisense lnc RNA DPP10-AS1 promotes lung cancer malignant processes and facilitates tumorigenesis by epigenetically regulating its cognate sense gene DPP10.DPP10-AS1 may serve as a candidate prognostic biomarker and a potential therapeutic target in lung cancer. | Haihua Tian Jinchang Pan Shuai Fang Chengwei Zhou Hui Tian Jinxian He Weiyu Shen Xiaodan Meng Xiaofeng Jin Zhaohui Gong | 2021 | Cancer Biology & Medicine2021，18，3： | 5 |
| 3 | Tribological Performance of Bioimplants: A Comprehensive Review显示文摘Total joint replacements(TJR) have been a huge success for orthopaedic surgery in the past century and are gaining increasing importance today due to the aging population. However, the short longevity of artificial joints is one of the major problems in bioimplant industry and needs to be rectified since an increasing number of young people, with more active lifestyles, must receive TJR. Wear mechanisms are discussed in this paper to describe the root causes of the failures and to give some general ideas to increase the lifespan of artificial joints. The suitable material combination is of great importance for the wear resistance of bioimplants, and bioceramics will exert a crucial effect in their future progress. Other materials, such as metal alloys and polymers, are also discussed in this paper. Surface finish is another factor affecting the tribological performance of bioimplants. In recent years, surface texture technology has fascinated many researchers, and a good design of texture pattern requires a comprehensive understanding of wear mechanisms, material properties, and dynamic fluid theory. This review also covers a summary of in vitro wear tests, including simulators, lubricant, and testing parameters. | Gang Shen Fengzhou Fang Chengwei Kang | 2018 | Nanotechnology and Precision Engineering2018，1，2： | 4 |
| 4 | Validation of the simplified criteria for diagnosis of autoimmune hepatitis in Chinese patients显示文摘 | Dekai Qiu Qixia Wang Hui Wang Qing Xie Guoqing Zang Hong Jiang Chuantao Tu Jinsheng Guo Shuncai Zhang Jianshe Wang Yi Lu Ying Han Lei Shen Xiaoyu Chen Xiqi Hu Xiaojin Wang Chengwei Chen Qingchun Fu Xiong Ma | 2010 | Journal of Hepatology2010，，： | 2 |
| 5 | HY5-HDA9 Module Transcriptionally Regulates Plant Autophagy in Response to Light-to-Dark Conversion and Nitrogen Starvation显示文摘(Molecular Plant 13,515-531;March 2020)After publication of our original manuscript,we became aware of errors in Figure 4.During the preparation of Figure 4C in this article as originally published,we inadvertently duplicated the image of hda9-1(MS-N)as that of the hda9-1-C(MS+N).Also,in Figure 4H,the image of pUBQ10:GFP-ATG8a/hda9-1(MS+L)was mistakenly a duplicate of pUBQ10:GFP-ATG8a/WT(MS+L)shown in Figure S7C.A corrected version of Figure 4 is shown below.The scientific conclusions of this article have not been affected by this correction.The authors apologize for not detecting this error prior to publication and for any inconvenience that may have been caused. | Chao Yang Wenjin Shen Lianming Yang Yun Sun Xibao Li Minyi Lai Juan Wei Chaojun Wang Yingchao Xu Faqiang Li Shan Liang Chengwei Yang Shangwei Zhong Ming Luo Caiji Gao | 2022 | Molecular Plant2022，15，10： | 0 |
| 6 | Cusp points and assembly changing motions in the PRR-PR-PRR planar parallel manipulator显示文摘Most parallel manipulators have multiple solutions to the direct kinematic problem.The ability to perform assembly changing motions has received the attention of a few researchers.Cusp points play an important role in the kinematic behavior.This study investigates the cusp points and assembly changing motions in a two degrees of freedom planar parallel manipulator.The direct kinematic problem of the manipulator yields a quartic polynomial equation.Each root in the equation determines the assembly configuration,and four solutions are obtained for a given set of actuated joint coordinates.By regarding the discriminant of the repeated roots of the quartic equation as an implicit function of two actuated joint coordinates,the direct kinematic singularity loci in the joint space are determined by the implicit function.Cusp points are then obtained by the intersection of a quadratic curve and a cubic curve.Two assembly changing motions by encircling different cusp points are highlighted,for each pair of solutions with the same sign of the determinants of the direct Jacobian matrices. | Chengwei SHEN Jingjun YU Xu PEI | 2023 | Frontiers of Mechanical Engineering2023，18，2： | 0 |
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