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| 1 | Latest research progress of marine microbiological corrosion and biofouling, and new approaches of marine anti-corrosion and anti-fouling显示文摘Marine resources and industry have become one of the most important pillars in economic development all over the world.However,corrosion of materials is always the most serious problem to the infrastructure and equipment served in marine environment.Researchers have found that microbiologically influenced corrosion(MIC)and marine bio-fouling are two main mechanisms of marine corrosions due to the complicated marine environment and marine organisms.This article summarized the latest research progress about these two mechanisms and indicated that both MIC and marine bio-fouling are closely related to the biofilms on material surfaces formed by the marine microorganisms and their metabolites.As a result,to prevent the occurrence of MIC and bio-fouling,it is important to control the microorganisms in biofilms or prevent the adhesion and formation of biofilms.The traditional method of using chemical bactericide or antifoulant faces the problems of pollution and microorganism resistance.This article introduced four research approaches about the new tendency of applying new materials and technologies to cooperate with traditional chemicals to achieve better and longer effects with lower environment pollution through synergistic actions.Finally,some future research tendencies were proposed for whole marine anti-corrosion and anti-fouling areas. | Yangfan Li Chengyun Ning | 2019 | Bioactive Materials2019，4，1： | 13 |
| 2 | Epidemiological Data of Work-Related Musculoskeletal Disorders—China,2018–2020显示文摘Summary What is already known about this topic?In recent decades,work-related musculoskeletal disorders(WMSDs)have become increasingly prominent and have become an important issue that is of universal concern and an urgent need to be solved in all countries of the world.What is added by this report?The top three industries or occupational groups with the highest standardized prevalence rate of WMSDs were flight attendants,medical staff,and vegetable greenhouses in that order.Women workers were 1.5 times more likely to suffer from WMSDs than men workers. | Ning Jia Huadong Zhang Ruijie Ling Yimin Liu Gang Li Zaoliang Ren Yan Yin Hua Shao Hengdong Zhang Bing Qiu Meibian Zhang Dayu Wang Qiang Zeng Rugang Wang Jianchao Chen Danying Zhang Liangying Mei Yongquan Liu Jixiang Liu Chengyun Zhang Tianlai Li Qing Xu Ying Qu Xueyan Zhang Xin Sun Zhongxu Wang | 2021 | China CDC weekly2021，3，18： | 13 |
| 3 | Exosome-functionalized polyetheretherketone-based implant with immunomodulatory property for enhancing osseointegration显示文摘The host immune response effecting on biomaterials is critical to determine implant fates and bone regeneration property.Bone marrow stem cells(BMSCs)derived exosomes(Exos)contain multiple biosignal molecules and have been demonstrated to exhibit immunomodulatory functions.Herein,we develop a BMSC-derived Exos-functionalized implant to accelerate bone integration by immunoregulation.BMSC-derived Exos were reversibly incorporated on tannic acid(TA)modified sulfonated polyetheretherketone(SPEEK)via the strong interaction of TA with biomacromolecules.The slowly released Exos from SPEEK can be phagocytosed by co-cultured cells,which could efficiently improve the biocompatibilities of SPEEK.In vitro results showed the Exos loaded SPEEK promoted macrophage M2 polarization via the NF-κB pathway to enhance BMSCs osteogenic differentiation.Further in vivo rat air-pouch model and rat femoral drilling model assessment of Exos loaded SPEEK revealed efficient macrophage M2 polarization,desirable new bone formation,and satisfactory osseointegration.Thus,BMSC-derived Exos-functionalized implant exerted osteoimmunomodulation effect to promote osteogenesis. | Lei Fan Pengfei Guan Cairong Xiao Huiquan Wen Qiyou Wang Can Liu Yian Luo Limin Ma Guoxin Tan Peng Yu Lei Zhou Chengyun Ning | 2021 | Bioactive Materials2021，6，9： | 11 |
| 4 | Projects of Earth Sciences Supported by National Natural Science Foundation of China显示文摘 | Guorong Xuan Qiuming Yao Chengyun Yang Jianjiong Gao Peiqi Chai et al. | 2001 | Journal of Geographical Sciences2001，11，1： | 11 |
| 5 | Investigation on Work-Related Musculoskeletal Disorders--China, 2018−2019显示文摘What is already known about this topic?Work-related musculoskeletal disorders(WMSDs)have a high prevalence and seriously harmful,which has attracted extensive attention in various countries in the world.Currently,the occurrence and rules of WMSDs in key industries are not known in China.What is added by this report?The prevalence of WMSDs is relatively high among professional populations in key industries in China,with the most commonly affected body parts concentrated in neck,shoulders,and low back and increasing with age and working years.What are the implications for public health practice?This study determined the prevalence and distribution characteristics of WMSDs in key industries in China and provided scientific evidence to recommend for inclusion of WMSDs in the new revision of the list of occupational diseases in China. | Ning Jia Huadong Zhang Ruijie Ling Yimin Liu Gang Li Zaoliang Ren Yan Yin Hua Shao Hengdong Zhang Bing Qiu Meibian Zhang Dayu Wang Qiang Zeng Rugang Wang Jianchao Chen Danying Zhang Liangying Mei Yongquan Liu Jixiang Liu Chengyun Zhang Tianlai Li Qing Xu Ying Qu Xueyan Zhang Xin Sun Zhongxu Wang | 2020 | China CDC weekly2020，2，18： | 11 |
| 6 | Platform for dynamic virtual auditory environment real-time rendering system显示文摘This paper reports the recent works and progress on a PC and C++ language-based virtual auditory environment(VAE) system platform.By tracing the temporary location and orientation of listener's head and dynamically simulating the acoustic propagation from sound source to two ears,the system is capable of recreating free-field virtual sources at various directions and distances as well as auditory perception in reflective environment via headphone presentation.Schemes for improving VAE performance,including PCA-based(principal components analysis) near-field virtual source synthesis,simulating six degrees of freedom of head movement,are proposed.Especially,the PCA-based scheme greatly reduces the computational cost of multiple virtual sources synthesis.Test demonstrates that the system exhibits improved performances as compared with some existing systems.It is able to simultaneously render up to 280 virtual sources using conventional scheme,and 4500 virtual sources using the PCA-based scheme.A set of psychoacoustic experiments also validate the performance of the system,and at the same time,provide some preliminary results on the research of binaural hearing.The functions of the VAE system is being extended and the system serves as a flexible and powerful platform for future binaural hearing researches and virtual reality applications. | ZHANG ChengYun XIE BoSun | 2013 | Chinese Science Bulletin2013，58，3： | 10 |
| 7 | Brassinosteroid Enhances Jasmonate-Induced Anthocyanin Accumulation in Arabidopsis Seedlings显示文摘Jasmonate (JA) regulates plant development, mediates defense responses, and induces anthocyanin biosynthesis as well. Previously, we isolated the psc1 mutant that partially suppressed coi1 insensitivity to JA, and found that brassinosteroid (BR) was involved in JA signaling and negatively regulated JA inhibition of root growth in Arabidopsis. In this study it was shown that JA-induced anthocyanin accumulation was reduced in BR mutants or in wild type treated with brassinazole, an inhibitor of BR biosynthesis, whereas it was induced by an application of exogenous BR. It was also shown that the'late'anthocyanin biosynthesis genes including DFR, LDOX , and UF3GT, were induced slightly by JA in the BR mutants relative to wild type. Furthermore, the expression level of JA-induced Myb/bHLH transcription factors such as PAP1, PAP2, and GL3, which are components of the WD-repeat/Myb/bHLH transcriptional complexes that mediate the 'late' anthocyanin biosynthesis genes, was lower in the BR mutants than that in wild type. These results suggested that BR affects JA-induced anthocyanin accumulation by regulating the 'late' anthocyanin biosynthesis genes and this regulation might be mediated by the WD-repeat/Myb/bHLH transcriptional complexes. | Zhihong Peng Chengyun Han Libing Yuan Kun Zhang Hongman Huang Chunmei Ren | 2011 | Journal of Integrative Plant Biology2011，53，8： | 9 |
| 8 | Corrosion mechanism of micro-arc oxidation treated biocompatible AZ31 magnesium alloy in simulated body fluid显示文摘The rapid degradation of magnesium(Mg) based alloys has prevented their further use in orthopedic trauma fixation and vascular intervention,and therefore it is essential to investigate the corrosion mechanism for improving the corrosion resistance of these alloys. In this work, the effect of applied voltage on the surface morphology and the corrosion behavior of micro-arc oxidation(MAO) with different voltages were carried out to obtain biocompatible ceramic coatings on AZ31 Mg alloy. The effects of applied voltage on the surface morphology and the corrosion behavior of MAO samples in the simulated body fluid(SBF) were studied systematically. Scanning electron microscope(SEM) and X-ray diffractometer(XRD)were employed to characterize the morphologies and phase compositions of coating before and after corrosion. The results showed that corrosion resistance of the MAO coating obtained at 250 V was better than the others in SBF. The dense layer of MAO coating and the corrosion precipitation were the key factors for corrosion behavior. The corrosion of precipitation Mg(OH)2and the calcium phosphate(Ca–P) minerals on the surface of MAO coatings could enhance their corrosion resistance effectively. In addition, the mechanism of MAO coated Mg alloys was proposed. | Ying Li Fang Lu Honglong Li Wenjun Zhu Haobo Pan Guoxin Tan Yonghua Lao Chengyun Ning Guoxin Ni | 2014 | Progress in Natural Science:Materials International2014，24，5： | 7 |
| 9 | Promoting Bone Mesenchymal Stem Cells and Inhibiting Bacterial Adhesion of Acid-Etched Nanostructured Titanium by Ultraviolet Functionalization显示文摘Titanium(Ti) and its alloys are used extensively in orthopedic implants because of their excellent biocompatibility,mechanical properties and corrosion resistance. However,titanium-based implant materials face many severe complications,such as implant loosening due to poor osseointegration and bacterial infections,which may lead to implant failure. Hence,preparing a biomaterial surface,which enhances the interactions with host cells and inhibits bacterial adhesion,may be an optimal strategy to reduce the incidence of implant failure. This study aims to improve osseointegration and confer antibacterial properties on Ti through a combination of two surface modifications including nanostructuring generated by acid etching and ultraviolet(UV) light treatment.Our results showed that without UV treatment,the acid etching treatment of Ti surface was effective at both improving the adhesion of bone mesenchymal stem cells(BMSCs) and increasing bacterial adhesion. A further UV treatment of the acid-etched surface however,not only significantly improved the cell adhesion but also inhibited bacterial adhesion. The acid-etched nanostructured titanium with UV treatment also showed a significant enhancement on cell proliferation,alkaline phosphatase(ALP) activity and mineralization. These results suggest that such nanostructured materials with UV treatment can be expected to have a good potential in orthopedic applications. | Guobo Lan Mei Li Ying Tan Lihua Li Xiaoming Yang Limin Ma Qingshui Yin Hong Xia Yu Zhang Guoxin Tan Chengyun Ning | 2015 | Journal of Materials Science & Technology2015，31，2： | 7 |
| 10 | Characterization of bioactive ceramic coatings prepared on titanium implants by micro-arc oxidation显示文摘微弧的氧化(毛) 是在电解质媒介的提高的化学工艺在阀门金属表面上获得涂层结构。毛在磷酸钠电解质获得的三氧化钛电影被调查。这些电影主要由在锐钛矿和金红石形式的 TiO_2 阶段组成并且在表面与 Na 和 P 元素充实。他们的导致磷灰石的能力在模仿的身体液体(SBF ) 被评估。在为超过 30 d 的 SBF 的 Whenimmersing,优先的 carbonated-hydroxyapatite 在这些电影的表面上被形成,它建议对待 MAO 的钛有有希望的积极生物回答。 | WANG Yingjun NAN Kaihui CHEN Xiaofeng NING Chengyun WANG lingyu ZHAO Naru | 2006 | Rare Metals2006，25，1： | 5 |
| 11 | Arabidopsis EXO70A1 recruits Patellin3 to the cell membrane independent of its role as an exocyst subunit显示文摘The exocyst is a well-known complex which tethers vesicles at the cell membrane before fusion. Whether an individual subunit can execute a unique function is largely unknown. Using yeast-two-hybrid(Y2H) analysis, we found that EXO70A1 interacted with the GOLD domain of Patellin3(PATL3). The direct EXO70A1-PATL3 interaction was supported by in vitro and in vivo experiments. In Arabidopsis, PATL3-GFP colocalized with EXO70A1 predominantly at the cell membrane, and PATL3 localization was insensitive to BFA and Try A23. Remarkably, in the exo70 a1 mutant, PATL3 proteins accumulated as punctate structures within the cytosol, which did not colocalize with several endomembrane compartment markers, and was insensitive to BFA. Furthermore, PATL3 localization was not changed in the exo70 e2, PRsec6 or exo84b mutants. These data suggested that EXO70A1, but not other exocyst subunits, was responsible for PATL3 localization,which is independent of its role in secretory/recycling vesicletethering/fusion. Both EXO70A1 and PATL3 were shown to bind PI4 P and PI(4,5)P2 in vitro. Evidence was obtained that the other four members of the PATL family bound to EXO70A1 as well, and shared a similar localization pattern as PATL3.These findings offered new insights into exocyst subunitspecific function, and provided data and tools for further characterization of PATL family proteins. | Chengyun Wu Lu Tan Max van Hooren Xiaoyun Tan Feng Liu Yan Li Yanxue Zhao Bingxuan Li Qingchen Rui Teun Munnik Yiqun Bao | 2017 | Journal of Integrative Plant Biology2017，59，12： | 3 |
| 12 | Self‐suspended rare‐earth doped up‐conversion luminescent waveguide:propagating and directional radiation显示文摘Near-infrared excited rare-earth(RE)-doped up-conversion(UC)-luminescent materials have attracted enormous attention because of their unique emission properties,such as narrow emission bands,long luminescence lifetimes,and multiple colors.However,current development of RE-doped luminescent material is hindered by weak and narrowband absorption problems and low photon-conversion quantum efficiencies.In addition to conventional approaches to enhance fluorescence intensity,controlling emission directivity to improve detection efficiency has become a promising approach to obtain higher luminescence brightnesses.In this paper,a self-suspended RE-doped UC luminescent waveguide is designed to realize directional emissions.Benefitting from the special morphology of the crown-like NaYF4:Yb3+/Er3+microparticle,the points contact between the waveguide and substrate can be obtained to decrease energy loss.An attractive UC luminescent pattern accompanied by powerful and controllable directional emissions is observed,and the spatial emission angle and intensity distribution are explored and analyzed in detail by introducing Fourier imaging detection and simulation.This work provides a new method for achieving controllable directional fluorescence emissions and obtaining improved detection efficiency by narrowing emission directivity,which has potential applications in 3-dimensional displays and micro-optoelectronic devices,especially when fabricating self-fluorescence micron lasers. | Changjian Zhang Chengyun Zhang Zhenglong Zhang Tao He Xiaohu Mi Ting Kong Zhengkun Fu Hairong Zheng Hongxing Xu | 2020 | Opto-Electronic Advances2020，3，6： | 3 |
| 13 | The “1-3-7” Approach to Malaria Surveillance and Response--Henan Province, China, 2012−2018显示文摘What is already known about this topic?The“1-3-7”approach to malaria surveillance and response was a key measure for malaria elimination in China and was first introduced into the World Health Organization(WHO)as an international guideline for malaria surveillance and response in 2018.What is added by this report?The“1-3-7”approach was well implemented in Henan Province from 2012−2018.Over this study period,a total of 1,294 malaria cases were detected and reported,and all cases were diagnosed and reported within 1 day with 99.23%(1,284/1,294)of cases were investigated within 3 days.In addition,93.7%(1,212/1,294)of foci were investigated and vector control was implemented within 7 days at all residual non-active foci to prevent further spread.What are the implications for public health practice?The“1-3-7”controlling pattern would be an effective and approachable method for implementation especially in malaria-eliminating countries and regions,but the interval from symptom onset to diagnosis cannot be ignored.Thus,the roles and responsibilities that all actors involved in the health sector must be specified too. | Qunqun Zhang Ying Liu Yabo Hu Yuling Zhao Chengyun Yang Dan Qian Ruimin Zhou Suhua Li Zhou Guan Deling Lu Hongwei Zhang Wanshen Guo | 2020 | China CDC weekly2020，2，17： | 3 |
| 14 | DNA alkylation promoted by an electron-rich quinone methide intermediate显示文摘生物申请结合源于 oligonucleotides, quinone methides 以前由他们自我使内收的共有原子价和目标 nucleic 酸的随后的完化的慢交换是有限的。为了提高这些的率,处理,有捐赠的一个电子的一位新 quinone methide 先锋取代者被准备了。另外,这个取代者被放了帕拉到为最大的效果的 quinone methide 的初生的 exo 甲又组。A 结合用这位先锋和 5-aminohexyloligonucleotide 做了加速形成它的可逆自我使内收并且它从优先的模型预言了的互补 DNA 的完化学习。 | Chengyun Huang Steven E. Rokita | 2016 | Frontiers of Chemical Science and Engineering2016，10，2： | 2 |
| 15 | Preparation and properties of a cerium-containing hydroxyapatite coating on commercially pure titanium by micro-arc oxidation显示文摘商业地纯的钛上的多孔的包含铈的 hydroxyapatite 涂层被微弧的氧化(毛) 在包含钙醋酸盐的一个电解答案准备,甘油磷酸盐 disodium 盐五水合物(-GP), 和铈硝酸盐。氧化物涂层的厚度,阶段,作文形态学,和 biocompatibility 被 X 光检查衍射(XRD ) 描绘,电子探查微量分析(EPMA ) ,与精力扫描电子显微镜学(SEM ) 散 X 光检查分光计(版本) ,和房间文化。毛电影的厚度关于 15-25 m,并且涂层多孔、不平,没有到钛底层的任何明显的接口。XRD 和版本的结果证明多孔的涂层由 hydroxyapatite 组成(哈) 电影包含 Ce。有利造骨细胞房间亲密关系做 Ce -- 哈电影有好 biocompatibility。Ce -- 哈电影被期望作为牙齿有重要医药应用植入并且人工的骨头关节。 | HUANG Yong WANG Yingjun NING Chengyun NAN Kaihui HAN Yong | 2008 | Rare Metals2008，27，3： | 2 |
| 16 | An injectable,self-healing,electroconductive extracellular matrix-based hydrogel for enhancing tissue repair after traumatic spinal cord injury显示文摘Injectable biomaterial-based treatment is a promising strategy to enhance tissue repair after traumatic spinal cord injury(SCI)by bridging cavity spaces.However,there are limited reports of injectable,electroconductive hydrogels with self-healing properties being employed for the treatment of traumatic SCI.Hence,a natural extracellular matrix(ECM)biopolymer(chondroitin sulphate and gelatin)-based hydrogel containing polypyrrole,which imparted electroconductive properties,is developed for traumatic SCI repair.The resulting hydrogels showed mechanical(~928 Pa)and conductive properties(4.49 mS/cm)similar to natural spinal cord tissues.Moreover,the hydrogels exhibited shear-thinning and self-healing abilities,which allows it to be effectively injected into the injury site and to fill the lesion cavity to accelerate the tissue repair of traumatic SCI.In vitro,electroconductive ECM hydrogels promoted neuronal differentiation,enhanced axon outgrowth,and inhibited astrocyte differentiation.The electroconductive ECM hydrogel activated endogenous neural stem cell neurogenesis in vivo(n=6),and induced myelinated axon regeneration into the lesion site via activation of the PI3K/AKT and MEK/ERK pathways,thereby achieving significant locomotor function restoration in rats with spinal cord injury(p<0.001,compared to SCI group).Overall,the injectable self-healing electroconductive ECM-based hydrogels developed in this study are ideal biomaterials for treatment of traumatic SCI. | Yian Luo Lei Fan Can Liu Huiquan Wen Shihuan Wang Pengfei Guan Dafu Chen Chengyun Ning Lei Zhou Guoxin Tan | 2022 | Bioactive Materials2022，7，1： | 2 |
| 17 | Erythropoiesis-stimulating agents in the management of cancer patients with anemia: a meta-analysis显示文摘Background: Erythropoiesis-stimulating agents(ESAs) are widely used in the management of anemia in cancer patients. Despite their apparent effectiveness, recent studies have suggested that ESAs could result in serious adverse events and even higher mortality. The aim of the current study was to evaluate the benefits and risks of ESAs in the management of cancer patients with anemia using a meta-analysis.Methods: The initial literature search covered Medline, PubMed, Embase, and the Cochrane Center Register of Controlled Trials, and identified 1,569 articles. The final meta-analysis included eight randomized controlled trials(n=2,387) in cancer patients with <11 g/dL hemoglobin(Hb) at the baseline and target Hb(for stopping ESA treatment) at no more than 13 g/dL. The assessment measures included Hb response, blood transfusion rate and adverse events that included venous thromboemblism(VTE), hypertension, and on-study mortality. The results are expressed as pooled odds ratio(OR). Publication bias was assessed using funnel plot analysis.Results: ESAs significantly increased the Hb concentration [OR 7.85, 95% confidence interval(CI): 5.85 to 10.53, P<0.001] and reduced the red blood cell(RBC) transfusion rate(OR 0.52, 95% CI: 0.42 to 0.65, P<0.001). ESAs did not increase the accumulated adverse events(OR 0.95, P=0.82), or the on-study mortality(OR 1.09, P=0.47).Conclusions: ESAs are not associated with increased frequency of severe adverse events in anemic cancer patients when the target Hb value is no more than 13 g/dL. | Xiaomei Li Zhi Yan Dexiao Kong Wen Zou Jihua Wang Dianshui Sun Yuhua Jiang Chengyun Zheng | 2014 | Chinese Journal of Cancer Research2014，26，3： | 2 |
| 18 | Controllable Protein Adsorption and Bacterial Adhesion on Polypyrrole Nanocone Arrays显示文摘In this research, polypyrrole nanocone arrays doped with β-Naphthalene sulphonic acid(PPy-NSA) were built. This film was expected to control protein adsorption and bacterial adhesion by potential-induced reversibly redox. The scanning Kelvin probe microscopy(SKPM) and surface contact angles(SCA) tests suggested that the surface potential and wettability of PPy-NSA nanocone arrays could be controlled by simply controlling its redox property via applying potential. The controllable surface potential and wettability in return controlled the adsorption of protein and adhesion of bacteria. The proposed material might find application in the preparation of smart biomaterial surfaces that can regulate proteins and bacterial adhesion by a simple potential switching. | Zhengnan Zhou Weiping Li Tianrui He Peng Yu Guoxin Tan Chengyun Ning | 2016 | Journal of Materials Science & Technology2016，32，9： | 2 |
| 19 | Dynamical influence of the Madden-Julian oscillation on the Northern Hemisphere mesosphere during the boreal winter显示文摘This study first investigates the effect of the Madden-Julian Oscillation(MJO)on the Northern Hemisphere(NH)mesosphere.Both observations and simulations suggest significant cooling in the NH polar mesosphere approximately 35 days after MJO phase 4(P4),which lags the MJO-induced perturbation in the upper stratosphere by 10 days.The enhanced planetary waves(PWs)propagate upward and result in wavenumber-1 pattern temperature anomalies in the mesosphere lagging MJO P4 by 25 days.The anomalous PWs also lead to the weaker eastward zonal wind in the upper stratosphere and lower mesosphere lagging MJO P4 by 30 days.Simultaneously,the weaker westerlies result in weaker climatological westward gravity waves(GWs)in the mesosphere due to critical-level filtering.The mesosphere meridional circulation is suppressed due to both anomalous PWs and GWs,and this suppression causes polar mesospheric cooling lagging MJO P4 by 35 days. | Cong SUN Chengyun YANG Tao LI | 2021 | Science China Earth Sciences2021，64，8： | 2 |
| 20 | The function of three indigenous plasmids in Mesorhizobium huakuii 2020 and its symbiotic inter-action with Sym pJB5JI of Rhizobium leguminosarum显示文摘A Mesorhizobium huakuii strain 2020, isolated from a rice-growing field in southern China, contains three indigenous plasmids named p2020a, p2020b and p2020c, respectively. The plasmids were deleted via Tn5-sacB insertion, and two cured derivatives were obtained. Interestingly, the mutant 2020D29 curing of p2020c could significantly enhance the capacity of symbiotic nitrogen fixation. But the mutant 2020D8 curing of p2020b lost the ability to nodulate Astragalus sinicus. Furthermore, the third plasmid p2020a could be hardly eliminated, suggesting that some house-keeping genes necessary for strain growth located on this plasmid. Then the Sym plasmid pJB5JI of R. leguminosarum bv. viciae was transferred into 2020 and its cured derivatives. The pot plant test showed that the ability of competition and symbiotic nitrogen fixation of transconjugant 2020-137 (pJB5JI) was increased evidently in con-trast to 2020. pJB5JI could not restore the ability of 2020D8 to nodulate Astragalus sinicus. 2020D8-8 (pJB5JI) could form ineffective nodules on peas, which implied that the symbiotic plasmid pJB5JI could express its function at the chromosomal background of Mesorhizobium huakuii 2020. The plas-mid stability was checked in transconjugants under free-living and during symbiosis. The results indi-cated that pJB5JI failed to be detected in some nodule isolates. That Km resistance gene could be am-plified from all transconjugants and nodule isolates suggested that pJB5JI was fully or partially inte-grated into the chromosome of recipients. | YANG ChengYun, LI YouGuo, WEI Li, CHENG GuoJun & ZHOU JunChu State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China | 2008 | Science China(Life Sciences)2008，51，4： | 2 |
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