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5篇 您的检索式：作者名="Chengzhu Liang"

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		题名	作者	年代	出处	被引量
	1	Detection of the End Point Temperature of Thermal Denatured Protein in Fish and Chicken Meat Through SDS-PAGE Electrophoresis显示文摘Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65 ℃ to 75 ℃ , and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated heating thermal denatured proteins under the same temperature and processing time were studied. The electrophoresis patterns of thermal denatured proteins determined through repeated heating at the same temperature did not exhibit any change. For the detection of cooked fish and meat samples, they were subjected to applying the SDS-PAGE method, which revealed an EPT ranging from 60 ℃ to 80 ℃ .	GAO Hongwei MAO Mao LIANG Chengzhu LIN Chao XIANG Jianhai	2009	Journal of Ocean University of China2009，8，1：	4
	2	Development and Characterization of Microsatellite Markers for the Pacific Abalone (Haliotis discus) via EST Database Mining显示文摘The EST database of the Pacific abalone (Haliotis discus) was mined for developing microsatellite markers. A total of 1476 EST sequences were registered in GenBank when data mining was performed. Fifty sequences (approximately 3.4%) were found to contain one or more microsatellites. Based on the length and GC content of the flanking regions, cluster analysis and BLASTN, 13 microsatellite-containing ESTs were selected for PCR primer design. The results showed that 10 out of 13 primer pairs could amplify scorable PCR products and showed polymorphism. The number of alleles ranged from 2 to 13 and the values of Ho and He varied from 0.1222 to 0.8611 and 0.2449 to 0.9311, respectively. No significant linkage disequilibrium (LD) between any pairs of these loci was found, and 6 of 10 loci conformed to the Hardy-Weinberg equilibrium (HWE). These EST-SSRs are therefore potential tools for studies of intraspecies variation and hybrid identification.	ZHAN Aibin BAO Zhenmin WANG Mingling CHANG Dan YUAN Jian WANG Xiaolong HU Xiaoli LIANG Chengzhu HU Jingjie	2008	Journal of Ocean University of China2008，7，2：	2
	3	Development of a sensitive and quantitative assay for spring viremia of carp virus based on real-time RT-PCR显示文摘	Zhiqin Yue Yong Teng Chengzhu Liang		0，，1：	1
	4	Application of loop-mediated isothermal amplification for detection of Yersinia enterocolitica in pork meat显示文摘	Hongwei Gao Zhiwen Lei Juntao Jia Shufeng Wang Ying Chen Min Sun Chengzhu Liang	2009	Journal of Microbiological Methods2009，，2：	1
	5	Development of a sensitive and quanlitative assay for spring viremia of carp virus based on real-time RT-PCR 显示文摘	Zhiqin Yue Yong Teng Chengzhu Liang	2008	J Virological Methods2008，152，：	1

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