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| 1 | High efficiency production of ginsenoside compound K by catalyzing ginsenoside Rb1 using snailase显示文摘The rare ginsenoside Compound K(C-K) is attracting more attention because of its good physiological activity and urgent need. There are many pathways to obtain ginsenoside C-K, including chemical and biological methods. Among these, the conversion of PPD-type ginsenosides by enzymatic hydrolysis is a trend due to its high efficiency and mild conditions. For effectively extracting from the other panaxadiol saponins, the conversion process for ginsenoside C-K was investigated using snailases in this study. The univariate experimental design and response surface methodology were used to determine the optimal hydrolysis conditions for the conversion of ginsenoside Rb1 into ginsenoside C-K by snailases. The optimum conditions were as follows: p H 5.12, temperature 51 °C, ratio of snailase/substrate 0.21, and reaction time 48 h. On the basis of these parameters, the addition of 1.0 mmol·L^(-1) ferric ion was found to significantly improve the enzymolysis of snailases for the first time. With the above conditions, the maximum conversion rate reached 89.7%, suggesting that the process can obviously increase the yield of ginsenoside C-K. The bioassay tests indicated that the ginsenoside C-K showed anti-tumor activity in a series of tumor cell lines. Based on these results, we can conclude that the process of rare ginsenoside CK production by enzymolysis with snailase is feasible, efficient, and suitable for the industrial production and application. | Zhiguang Duan Chenhui Zhu Jingjing Shi Daidi Fan Jianjun Deng Rongzhan Fu Rong Huang Cuiying Fan | 2018 | Chinese Journal of Chemical Engineering2018，26，7： | 5 |
| 2 | Glucose and MMP-9 dual-responsive hydrogel with temperature sensitive self-adaptive shape and controlled drug release accelerates diabetic wound healing显示文摘Chronic diabetic wounds are an important healthcare challenge. High concentration glucose, high level of matrix metalloproteinase-9 (MMP-9), and long-term inflammation constitute the special wound environment of diabetic wounds. Tissue necrosis aggravates the formation of irregular wounds. All the above factors hinder the healing of chronic diabetic wounds. To solve these issues, a glucose and MMP-9 dual-response temperature-sensitive shape self-adaptive hydrogel (CBP/GMs@Cel&INS) was designed and constructed with polyvinyl alcohol (PVA) and chitosan grafted with phenylboric acid (CS-BA) by encapsulating insulin (INS) and gelatin microspheres con-taining celecoxib (GMs@Cel). Temperature-sensitive self-adaptive CBP/GMs@Cel&INS provides a new way to balance the fluid-like mobility (self-adapt to deep wounds quickly, approximately 37 ◦C) and solid-like elasticity (protect wounds against external forces, approximately 25 ◦C) of self-adaptive hydrogels, while simultaneously releasing insulin and celecoxib on-demand in the environment of high-level glucose and MMP-9. Moreover, CBP/ GMs@Cel&INS exhibits remodeling and self-healing properties, enhanced adhesion strength (39.65 ± 6.58 kPa), down-regulates MMP-9, and promotes cell proliferation, migration, and glucose consumption. In diabetic full-thickness skin defect models, CBP/GMs@Cel&INS significantly alleviates inflammation and regulates the local high-level glucose and MMP-9 in the wounds, and promotes wound healing effectively through the synergistic effect of temperature-sensitive shape-adaptive character and the dual-responsive system. | Wanyi Zhou Zhiguang Duan Jing Zhao Rongzhan Fu Chenhui Zhu Daidi Fan | 2022 | Bioactive Materials2022，7，11： | 4 |
| 3 | Construction of multifunctional hydrogel based on the tannic acid-metal coating decorated MoS2 dual nanozyme for bacteria-infected wound healing显示文摘Bacterial infection,tissue hypoxia and inflammatory response can hinder the infected wound repair process.To mitigate the above issues,tannic acid-chelated Fe-decorated molybdenum disulfide nanosheets(MoS2@TA/Fe NSs)with dual enzyme activities were developed and anchored to a multifunctional hydrogel.The hydrogel exhibited excellent antibacterial ability owing to the combined effects of photothermal therapy(PTT),glutathione(GSH)loss,and the peroxidase(POD)-like activity(catalyse H2O_(2)into⋅OH under acid condition)of MoS2@TA/Fe NSs.Benefitting from the catalase(CAT)-like activity,the hydrogel could decompose H2O_(2)into O_(2)at neutral pH to relieve hypoxia and supply adequate O_(2).POD-like activity was mainly attributed to MoS2 NSs,while CAT-like activity was primarily due to TA/Fe complex.Moreover,MoS2@TA/Fe NSs endowed the hydrogel with outstanding anti-oxidant ability to scavenge redundant reactive oxygen species(ROS)and reactive nitrogen species(RNS)under neutral environment to maintain the balance of antioxidant systems and prevent inflammation.In addition,the hydrogel could inhibit the release of inflammatory factors for the anti-inflammatory property of TA.TA retained partial phenolic hydroxyl groups,which cross-linked the nanosheets to the network structure of the hydrogel and promoted the adhesion of hydrogels.Due to the dynamic boron ester bonds between polyvinyl alcohol(PVA),dextran(Dex),MoS2@TA/Fe,and borax,the hydrogel demonstrated fast self-healing and rapid shape adaptability.This shape-adaptable adhesive hydrogel could fill the whole wound and closely contact the wound,ensuring that it achieved its functions with maximum efficiency.The MoS2@TA/Fe nanozyme-anchored multifunctional hydrogel showed high potential for bacteria-infected wound healing. | Yang Li Rongzhan Fu Zhiguang Duan Chenhui Zhu Daidi Fan | 2022 | Bioactive Materials2022，7，3： | 2 |
| 4 | RF-Net: Unsupervised Low-Light Image Enhancement Based on Retinex and Exposure Fusion显示文摘Low-light image enhancement methods have limitations in addressing issues such as color distortion,lack of vibrancy,and uneven light distribution and often require paired training data.To address these issues,we propose a two-stage unsupervised low-light image enhancement algorithm called Retinex and Exposure Fusion Network(RFNet),which can overcome the problems of over-enhancement of the high dynamic range and under-enhancement of the low dynamic range in existing enhancement algorithms.This algorithm can better manage the challenges brought about by complex environments in real-world scenarios by training with unpaired low-light images and regular-light images.In the first stage,we design a multi-scale feature extraction module based on Retinex theory,capable of extracting details and structural information at different scales to generate high-quality illumination and reflection images.In the second stage,an exposure image generator is designed through the camera response mechanism function to acquire exposure images containing more dark features,and the generated images are fused with the original input images to complete the low-light image enhancement.Experiments show the effectiveness and rationality of each module designed in this paper.And the method reconstructs the details of contrast and color distribution,outperforms the current state-of-the-art methods in both qualitative and quantitative metrics,and shows excellent performance in the real world. | Tian Ma Chenhui Fu Jiayi Yang Jiehui Zhang Chuyang Shang | 2023 | Computers, Materials & Continua2023，77，10： | 0 |
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