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| 1 | 8-bromo-7-methoxychrysin-induced apoptosis of hepatocellular carcinoma cells involves ROS and JNK显示文摘AIM:To investigate whether the apoptotic activities of 8-bromo-7-methoxychrysin(BrMC) involve reactive oxygen species(ROS) generation and c-Jun N-terminal kinase(JNK) activation in human hepatocellular carcinoma cells(HCC).METHODS:HepG2,Bel-7402 and L-02 cell lines were cultured in vitro and the apoptotic effects of BrMC were evaluated by flow cytometry(FCM) after propidium iodide(PI) staining,caspase-3 activity using enzymelinked immunosorbent assay(ELISA),and DNA agarose gel electrophoresis.ROS production was evaluated by FCM after dichlorodihydrofluorescein diacetate(DCHFDA) probe labeling.The phosphorylation level of JNK and c-Jun protein was analyzed by Western blotting.RESULTS:FCM after PI staining showed a dose-dependent increase in the percentage of the sub-G1 cell pop-ulation(P < 0.05),reaching 39.0% ± 2.8% of HepG2 cells after 48 h of treatment with BrMC at 10 μmol/L.The potency of BrMC to HepG2 and Bel-7402(32.1% ± 2.6%) cells was found to be more effective than the lead compound,chrysin(16.2% ± 1.6% for HepG2 cells and 11.0% ± 1.3% for Bel-7402 cell) at 40 μmol/L and similar to 5-flurouracil(33.0% ± 2.1% for HepG2 cells and 29.3% ± 2.3% for Bel-7402 cells) at 10 μmol/L.BrMC had little effect on human embryo liver L-02 cells,with the percentage of sub-G1 cell population 5.4% ± 1.8%.Treatment of HepG2 cells with BrMC for 48 h also increased the levels of active caspase-3,in a concentration-dependent manner.z-DEVD-fmk,a caspase-3specific inhibitor,prevented the activation of caspase-3.Treatment with BrMC at 10 μmol/L for 48 h resulted in the formation of a DNA ladder.Treatment of cells with BrMC(10 μmol/L) increased mean fluorescence intensity of DCHF-DA in HepG2 cells from 7.2 ± 1.12 at 0 h to 79.8 ± 3.9 at 3 h and 89.7 ± 4.7 at 6 h.BrMC did not affect ROS generation in L-02 cells.BrMC treatment failed to induce cell death and caspase-3 activation in HepG2 cells pretreated with N-acetylcysteine(10 mmol/L).In addition,in HepG2 cells treated with BrMC(2.5,5.0,10.0 μmol/L) for 12 h,JNK activation was observed.Peak JNK activation occurred at 12 h post-treatment and this activation persisted for up to 24 h.The expression of phosphorylated JNK and c-Jun protein after 12 h with BrMC-treated cells was inhibited by N-acetylcysteine and SP600125 pre-treatment,but GW9662 had no effect.SP600125 substantially reduced BrMC-induced cell death and caspase-3 activation of HepG2 cells.N-acetylcysteine and GW9662 also attenuated induction of cell death and caspase-3 activation in HepG2 cells treated with BrMC.CONCLUSION:BrMC induces apoptosis of HCC cells by ROS generation and sustained JNK activation. | Xiao-Hong Yang Jian-Guo Cao Hong-Lin Xiang Fei Liu Yuan Lv Hunan Province China Xing Zheng | 2010 | World Journal of Gastroenterology2010，16，27： | 38 |
| 2 | Transient Macular Edema after Laser In-Situ Keratomileusis显示文摘Purpose: To investigate the macular changes induced by laser in situ keratomileusisc(LASIK) procedure.Methods: Optical Coherence Tomography(OCT) was used to examine 27 eyes of 15LASIK patients before surgery, and 1 day, 1 week, 1 month after surgery.Results: The mean thick iness of neuroepithelial layer of macula was (195±24)microns, ( 178 ± 16) microns 1 day and 1 month after surgery, respectively, while thebaseline was (174 ± 12)microns. The increase in macular neuroepithelial thickness wascorrelated with the degree of myopia, but not with the duration of suction or thepostoperative BCVA.Conclusion: Mild macular edema is common in the first month following LASIK,especially in high myopia. However, no association with loss of BCVA has beenestablished. | Bin Yang, Zheng Wang, Guofu Huang, Xing Liu, Yunlan Ling, Xiaoping ZhengZhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou 510060, China | 2003 | Eye Science2003，19，1： | 10 |
| 3 | Time-frequency characteristics based motion estimation and imaging for high speed spinning targets via narrowband waveforms显示文摘This paper focuses on the motion parameters estimation and inversed synthetic aperture radar (ISAR) imaging for the high-speed spinning targets via the time-frequency characteristics. Assuming that the translational motion is compensated with the available motion compensation techniques, then the residual part of translational motion and the spinning motion are estimated with high precision based on the timefrequency characteristics. Combining with the procedure of the motion estimation, a novel coherent timefrequency spectrum intensity redistribution method, coherent single range Doppler interferometry (CSRDI), is proposed, which provides higher resolution and sustain lower signal to noise rate (SNR) than the current approach. Numerical simulation confirms the validity and high precision of the proposed approaches. | ZHANG Lei , LI YaChao, LIU Yan, XING MengDao & BAO Zheng National Lab for Radar Signal Processing, Xidian University, Xi’an 710071, China | 2010 | Science China(Information Sciences)2010，53，8： | 9 |
| 4 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 5 | A System Simulation Platform for 3G显示文摘A 3G system simulation platform is designed to research on and evaluate the effects of various techniques of the 3G network performance. The basic structure and principle of the simulation platform is presented and the simulation process is addressed in detail. | LU Zheng xing, YANG Da cheng (Wireless Research Center of Beijing University of Posts and Telecommunications, Beijing 100876, P. R. China) | 2003 | The Journal of China Universities of Posts and Telecommunications2003，10，3： | 4 |
| 6 | Trend and seasonal variations of atmospheric CH_4 in Beijing显示文摘The atmospheric CH\-4 in Beijing is still increasing, even though its increasing rate has significantly decreased from 1.76%/a during 1985—1989 to 0.50%/a during 1990—1997. The seasonal variation of CH\-4 concentration showed a double\|peak pattern, one peak appearing in winter and the other in summer. It is evident that the annually seasonal variations of atmospheric CH\-4 in Beijing are different. From 1986 to 1997, the atmospheric CH\-4 increased by 185 ppbv, 37% and 21% of which were due to the increase in winter and in summer, respectively. After 1993, the annually seasonal increasing rate of CH\-4 concentration in summer (due to emission from biogenic sources) is negative while the increasing rate in winter (due to emission from non\|biogenic sources) is positive about 25 ppbv/a. As a result, the increase of CH\-4 emission from non\|biogenic sources in winter is the major reason that caused the annually seasonal increasing rate from 1993 to 1997. The biogenic sources in Beijing are shrinking while the non\|biogenic ones (such as fossil fuel combustion) are enlarging. | WANG Yue\|si, WANG Ming\|xing, LUO Dong\|mei, ZHENG Xun\|hua, ZHOU Li (Institute of Atmospheric Physics, Chinese Academy of Sciences, Beijing 100029, China) | 2000 | Journal of Environmental Sciences2000，12，3： | 2 |
| 7 | A New Model for Predicting Ternary Thermodynamic Properties of Solution Phase and Its Application显示文摘A new geometric asymmetric model for predicting the thermodynamic properties of ternary systemfrom three sub-binaries is presented in this paper.Its analytical expression with good calculating ac-curacy is most simple in all asymmetric models.The new model was applied to predict the enthalpiesof mixing of the Bi-Ga-Sn.Au-Ag-Sn and NaCl-KCl-CaCl2 ternary systems which were in goodagreements with experimental data.Based on thermodynamic principles,the importance of thechoice of asymmetric component is also discussed.The error analysis indicates that in the case ofjudging the asymmetric component in ternary system according to the thermodynamic criterion thedeviation is minimum.It is of great significance to improve the calculating accuracy for predictingthermodynamic properties of ternary solution phase from the sub-binary ones. | Zhiyu QIAO Xianran XING Shuzhen DUAN University of Science and Technology Beijing,Beijing,100083,ChinaChaogui ZHENG Beijing University,Beijing,100871,China | 1993 | Journal of Materials Science & Technology1993，9，3： | 0 |
| 8 | Treatment and surveillance for non-muscle-invasive bladder cancer:a clinical practice guideline(2021 edition)显示文摘Non-muscle invasive bladder cancer(NMIBC)is a major type of bladder cancer with a high incidence worldwide,resulting in a great disease burden.Treatment and surveillance are the most important part of NIMBC management.In 2018,we issued“Treatment and surveillance for non-muscle-invasive bladder cancer in China:an evidencebased clinical practice guideline”.Since then,various studies on the treatment and surveillance of NMIBC have been published.There is a need to incorporate these materials and also to take into account the relatively limited medical resources in primary medical institutions in China.Developing a version of guideline which takes these two issues into account to promote the management of NMIBC is therefore indicated.We formed a working group of clinical experts and methodologists.Through questionnaire investigation of clinicians including primary medical institutions,24 clinically concerned issues,involving transurethral resection of bladder tumor(TURBT),intravesical chemotherapy and intravesical immunotherapy of NMIBC,and follow-up and surveillance of the NMIBC patients,were determined for this guideline.Researches and recommendations on the management of NMIBC in databases,guideline development professional societies and monographs were referred to,and the European Association of Urology was used to assess the certainty of generated recommendations.Finally,we issued 29 statements,among which 22 were strong recommendations,and 7 were weak recommendations.These recommendations cover the topics of TURBT,postoperative chemotherapy after TURBT,Bacillus Calmette–Guérin(BCG)immunotherapy after TURBT,combination treatment of BCG and chemotherapy after TURBT,treatment of carcinoma in situ,radical cystectomy,treatment of NMIBC recurrence,and follow-up and surveillance.We hope these recommendations can help promote the treatment and surveillance of NMIBC in China,especially for the primary medical institutions. | Ying-Hui Jin Xian-Tao Zeng Tong-Zu Liu Zhi-Ming Bai Zhong-Ling Dou De-Gang Ding Zhi-Lu Fan Ping Han Yi-Ran Huang Xing Huang Ming Li Xiao-Dong Li Yi-Ning Li Xu-Hui Li Chao-Zhao Liang Jiu-Min Liu Hong-Shun Ma Juan Qi Jia-Qi Shi Jian Wang De-Lin Wang Zhi-Ping Wang Yun-Yun Wang Yong-Bo Wang Qiang Wei Hai-Bo Xia Jin-Chun Xing Si-Yu Yan Xue-Pei Zhang Guo-You Zheng Nian-Zeng Xing Da-Lin He Xing-Huan Wang on behalf of the Chinese Urological Doctor Association(CUDA),Urological Association of Chinese Research Hospital Association(CRHA-UA),Uro-Health Promotive Association of China International Exchange,Promotive Association for Medical,Health Care(CPAM-UHPA) | 2023 | Military Medical Research2023，10，2： | 0 |
| 9 | Study on Stereometric Parameters of Optic Nerve Head of Normal, Big-cupped Disk and Glaucomatous Eyes Using Heidelberg Retina Tomograph显示文摘Purpose: To investigate the difference of stereometric parameters of optic nerve head between the normal subjects and patients with big-cupped disk and primary open angle glaucoma (POAG).Methods: Twenty-two cases (44 eyes) of normal subjects, 17 cases (34 eyes) of patients with big-cupped disk and 19 cases (37 eyes) of patients with POAG underwent Heidelberg Retina Tomograph (HRT) examination to get topography images and stereometric parameters of optic nerve head.Results: The stereometric parameters of optic nerve head of the normal, patients with big-cupped disk and POAG were 1) disk area (mm2): 1. 995± 0. 501, 2. 407±0. 661 and 2. 248±0.498; 2) cup area (mm2): 0.573±0.264, 1. 095±0. 673 and 1. 340±0. 516; 3) cup/disk ratio: 0. 25±0. 095, 0. 428±0. 176 and 0. 589±0.195; 4) rim area (mm2): 1.461±0.328, 1.312±0.418 and 0. 905± 0.409; 5)cup volume (mm3): 0. 108±0. 073, 0. 347±0. 346 and 0. 550 ±0. 394; 6) rim volume (mm3): 0. 421±0. 111, 0. 378±0. 225 and 0. 224±0. 189; 7) mean cup | Xing liu, Yunlan Ling, Xiaoping Zheng, Jingjing HuangZhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China | 2000 | 眼科学报2000，16，3： | 0 |
| 10 | Observation and nursing in rescuing organic phosphorus poisoning by active carbon显示文摘CLINICALINFOMATIONANDMETHODSThe42organicphosphoruspoisoned,16maleand26female,aged18~61,haveanaverageof34.6years.Thetoxicantar... | JIN Xiang cheng,CHEN Hao ming,HUO Xing na, ZHENG Mei,YU Mei fang,TANG Xin gong,WANG Tao Blood Purification Centre of Shaoxing Peoples Hospital,Zhejiang 312000,China | 1999 | Chinese Journal of Biomedical Engineering(English Edition)1999，8，1： | 0 |
| 11 | Evaluation of hepatitis B viral replication and proteomic analysis of HepG2.2.15 cell line after knockdown of HBx显示文摘BACKGROUND:Hepatitis B virus (HBV) is one of the major pathogens of human liver disease.Studies have shown that HBV X protein (HBx) plays an important role in promoting viral gene expression and replication.In this study we performed a global proteomic profiling to identify the downstream functional proteins of HBx,thereby detecting the mechanisms of action of HBx on virion replication.METHODS:HBx in the HepG2.2.15 cell line was knocked down by the transfection of small interfering RNA (siRNA).The replication level of HBV was evaluated by microparticle enzyme immunoassay analysis of HBsAg and HBeAg in the culture supernatant,and real-time quantitative PCR analysis of HBV DNA.Two-dimensional electrophoresis combined with MALDI-TOF/TOF was performed to analyze the changes in protein expression profile after treatment with HBx siRNA.RESULTS:Knockdown of HBx disturbed HBV replication in vitro.HBx target siRNA significantly inhibited the expression of HBsAg,HBeAg and the replication of HBV DNA.Twelve significantly changed proteins (7 upregulated and 5 downregulated) were successfully identified by MALDI-TOF/TOF using proteomics differential expression analysis after the knockdown of HBx.Among these identified proteins,HSP70 was validated by Western blotting.CONCLUSION:The results of the study indicated the positive effect of HBx on HBV replication,and a group of downstream target proteins of HBx may be responsible for this effect. | Hai-Yang Xie,Jun Cheng,Chun-Yang Xing,Jin-Jin Wang,Rong Su,Xu-Yong Wei,Lin Zhou and Shu-Sen Zheng Author Affiliations:Key Laboratory of Combined Multi-organ Transplantation,Ministry of Public Health Key Laboratory of Organ Transplantation Zhejiang Province and Division of Hepatobiliary and Pancreatic Surgery,Department of Surgery,First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China | 2011 | Hepatobiliary & Pancreatic Diseases International2011，10，3： | 0 |
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