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| 1 | Ultrafast photonic PCR显示文摘Nucleic acid amplification and quantification via polymerase chain reaction(PCR)is one of the most sensitive and powerful tools for clinical laboratories,precision medicine,personalized medicine,agricultural science,forensic science and environmental science.Ultrafast multiplex PCR,characterized by low power consumption,compact size and simple operation,is ideal for timely diagnosis at the point-of-care(POC).Although several fast/ultrafast PCR methods have been proposed,the use of a simple and robust PCR thermal cycler remains challenging for POC testing.Here,we present an ultrafast photonic PCR method using plasmonic photothermal light-to-heat conversion via photon–electron–phonon coupling.We demonstrate an efficient photonic heat converter using a thin gold(Au)film due to its plasmon-assisted high optical absorption(approximately 65%at 450 nm,the peak wavelength of heat source light-emitting diodes(LEDs)).The plasmon-excited Au film is capable of rapidly heating the surrounding solution to over 150℃ within 3 min.Using this method,ultrafast thermal cycling(30 cycles;heating and cooling rate of 12.7960.93℃ s^(-1) and 6.660.29℃ s^(-1),respectively)from 55℃(temperature of annealing)to 95℃(temperature of denaturation)is accomplished within 5 min.Using photonic PCR thermal cycles,we demonstrate here successful nucleic acid(λ-DNA)amplification.Our simple,robust and low cost approach to ultrafast PCR using an efficient photonic-based heating procedure could be generally integrated into a variety of devices or procedures,including on-chip thermal lysis and heating for isothermal amplifications. | Jun Ho Son Byungrae Cho SoonGweon Hong Sang Hun Lee Ori Hoxha Amanda J Haack Luke P Lee | 2015 | Light(Science & Applications)2015，4，1： | 2 |
| 2 | Sensitivity Analysis for Strongly Nonlinear Quasi-Variational Inclusions 显示文摘 | Agarwal B P CHo Y J Huang N J | 2000 | Appl Math Lett2000，13，： | 2 |
| 3 | Stability of Iterative Procedures with Errors Approximating Common Fixed Points for Qui-Contractive Mapping in q-Uniformly Smooth Banach Spaces 显示文摘 | Agaral R P Cho Y J Li J Huang Nan-jing | 2002 | Math Anal Appl2002，22，： | 1 |
| 4 | Lipopolysaccharideinducible nitric oxide synthase expression by dibenzylbutyrolactone lignans through inhibition of I-κBα phosphorylation and of p65 nuclear translocation in macrophages 显示文摘 | Cho M K Park J W Jang Y P | 2002 | Int Immunopharmacol2002，67，6： | 1 |
| 5 | The pi40 gene for durable resistance to rice blast and molecular analysis of pi40-advanced backcross breeding lines显示文摘 | Sub J P Roh J H Cho Y C | 2009 | Phytopathology2009，99，3： | 1 |
| 6 | An 80-kilodalton protein that binds to the pre-s1 domain of hepatitis B virus显示文摘 | Ryu C J Cho D Y Gripon P | 2000 | Virology2000，74，1： | 1 |
| 7 | A previously uneharaeterized role for small protein B (SmpB) in transfer messenger RNA-mediated trans-translation 显示文摘 | Sundermeier T R Dulebohn D P Cho H J | 2005 | Proe Natl Aead Sei U S A2005，102，7： | 1 |
| 8 | The Madin darbycanine Kidney ( MDCK) epithelial cell monolayer as a model cel-lular transport barrier显示文摘 | Cho M J Thompson D P Cramer C T | 1989 | Pharm Res1989，6，1： | 1 |
| 9 | The study of deNOx catalyst in low temperature using nano-sized supports显示文摘 | Lee J Y Hong S H Cho S P | 2006 | Current Applied Physics2006，6，： | 1 |
| 10 | Edible films made from membrane processed soy protein concentrates显示文摘 | Cho S Y Park J W Batt H P | 2007 | LWT-Food Science and Technology2007，40，3： | 1 |
| 11 | Cloning and eharaeterization of a flavonol synthase gene fromscutellaria baicalensis 显示文摘 | Kiln Y B Kim K Kim Y Tuan P A Kim H H Cho J Park S U | 2014 | Scientific World Journal2014，2014，98： | 1 |
| 12 | Drug release mechanism of paclitaxel from a chitosan-lipid implant system:effect of swelling,degradation and morphology显示文摘 | Lim Soo P Cho J Grant J | 2008 | Eur J Pharm Biopharm2008，69，1： | 1 |
| 13 | Closed-Loop Bias Control of Optical Quadrature Modulator 显示文摘 | Cho P S Khurgin J B Shpantzer I | 2006 | Photon- ics Technology Letters IEEE2006，18，21： | 1 |
| 14 | Enhaneed diffusion in polymer electrolyte membrane fuel cells using oscillating flow 显示文摘 | HwangY Lee D Choi J W Kim S Cho S H Joonho P Kim M S Jang J H Kim S H Cha S | 2010 | International Journal of Hydrogen Energy2010，35，8： | 1 |
| 15 | The Volatilization of Organic Compounds in Unsaturated Porous Media during Infiltration显示文摘 | Cho H J Jaffe P R | 1990 | Journal of Contaminant Hydrology1990，，6： | 1 |
| 16 | Genetic susceptibility factors on genes involved in the steroid hormone biosynthesis pathway and progesterone receptor for gastric cancer risk 显示文摘 | Cho L Y Yang J J Ko K P | 2012 | PLoS One2012，7，47： | 1 |
| 17 | Highly Uniform Growth of Monolayer Graphene by Chemical Vapor Deposition on Cu-Ag Alloy Catalysts 显示文摘 | Ryu J Cho S P Lee E K | 2014 | Physical Chemistry Chemical Physics2014，16，7： | 1 |
| 18 | Distinct roles of glycogen synthase kinase (GSK) 3alpha and GSK-3beta in mediating cardiomyocyte differentiation in murine bone marrow-derived mesenchymal stem cells 显示文摘 | Cho J Rameshwar P Sadoshima J | 2009 | J Biol Chem2009，284，26： | 1 |
| 19 | Carbon-based Novel Sorbent for Removing Gas-Phase Mercury显示文摘 | Lee S H Rhim Y J Cho S P | 2006 | Fuel2006，，85： | 1 |
| 20 | A study on the corrosion behavior austenitic stainless steel in hot molten salt of LiCl and LiCl-Li2O显示文摘 | Zhang J S Shins P K | 1999 | Korean Journal of Mater Research1999，9，2： | 1 |
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