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| 1 | 一种高效便捷的水稻DNA提取法及其应用显示文摘以水稻叶片、根和种子为材料,采用高通量快速法提取基因组DNA,用稻瘟病Pita基因分子标记进行检测,获得与预期片段大小一致的特异性条带,对165份育种材料的检测结果与稻瘟病接种鉴定一致。操作方法如下:将少量水稻叶片、根或种子放入200μL PCR盘中,加入70μL缓冲液A(含NaOH和Tween20),在PCR仪中加热到95℃,保持10min,再加入70μL缓冲液B(Tris-HCl和EDTA),该提取液可以直接用于PCR扩增。该方法具有几个优点:1)成本低,仅用4种化学试剂,共140μL提取液;2)操作简便,仅需3步,每人每天可以提取上千份样品;3)仪器设备简单,只用常规的PCR仪;4)可直接提取干种子的DNA;5)DNA质量好,能检测出水稻中的抗稻瘟病单基因Pita,并且与稻瘟病接种鉴定结果一致;6)用量少,只需要5~20mg叶片、20mg根或半粒籽粒。尤其是检测大量样本的基因型时,此种方法更显得高效便捷。 | 赵国珍 贾育林 严宗卜 Christopher W DEREN Melissa H JIA 戴陆园 | 2012 | 中国水稻科学2012，26，4： | 21 |
| 2 | Paraesophageal hernia and iron deficiency anemia:Mechanisms,diagnostics and therapy显示文摘There is ample clinical evidence suggesting that the presence of large axial or paraesophageal hernias may lead to iron deficiency anemia.So-called Cameron lesions,as well as other small mucosa erosions,in the sliding area of these diaphragmatic hernias lead to invisible chronic blood loss and consequently to iron depletion.While the spectrum of symptoms in these patients is large,anemia is often not the only indication and typically not the primary indication for surgical correction of diaphragmatic hernias.Drug treatment with proton pump inhibitors and iron substitution can alleviate anemia,but this is not always successful.To exclude other possible bleeding sources in the gastrointestinal tract,a comprehensive diagnostic program is necessary and reviewed in this manuscript.Additionally,we discuss controversies in the surgical management of paraesophageal hernias. | Christoph G Dietrich Dolores Hübner Joachim W Heise | 2021 | World Journal of Gastrointestinal Surgery2021，13，3： | 2 |
| 3 | Emission from quantum-dot high-β microcavities: transition from spontaneous emission to lasing and the effects of superradiant emitter coupling显示文摘Measured and calculated results are presented for the emission properties of a new class of emitters operating in the cavity quantum electrodynamics regime.The structures are based on high-finesse GaAs/AlAs micropillar cavities,each with an active medium consisting of a layer of InGaAs quantum dots(QDs)and the distinguishing feature of having a substantial fraction of spontaneous emission channeled into one cavity mode(highβ-factor).This paper demonstrates that the usual criterion for lasing with a conventional(lowβ-factor)cavity,that is,a sharp non-linearity in the input–output curve accompanied by noticeable linewidth narrowing,has to be reinforced by the equal-time second-order photon autocorrelation function to confirm lasing.The paper also shows that the equal-time second-order photon autocorrelation function is useful for recognizing superradiance,a manifestation of the correlations possible in high-βmicrocavities operating with QDs.In terms of consolidating the collected data and identifying the physics underlying laser action,both theory and experiment suggest a sole dependence on intracavity photon number.Evidence for this assertion comes from all our measured and calculated data on emission coherence and fluctuation,for devices ranging from light-emitting diodes(LEDs)and cavity-enhanced LEDs to lasers,lying on the same two curves:one for linewidth narrowing versus intracavity photon number and the other for g(2)(0)versus intracavity photon number. | Sören Kreinberg Weng W Chow Janik Wolters Christian Schneider Christopher Gies Frank Jahnke Sven Höfling Martin Kamp Stephan Reitzenstein 无 | 2017 | Light(Science & Applications)2017，6，1： | 2 |
| 4 | Bacterial RNA chaperones confer abiotic stress tolerance in plants and improved grain yield in maize under water-limited condition 显示文摘 | Paolo C Dave W Robert J B Don C A Jay H Martin S Mark A Ganesh K Sara S Robert D Santiago N Stephanie B Mary F Jayaprakash T Santanu D Christopher B Michael H L Jacqueline E H | 2008 | Plant Physiology2008，147，2： | 1 |
| 5 | Knowl edge network characteristics and entrepreneurial orienta tion of multinational corporations, research policy显示文摘 | CHRISTOPHER W SOO H L RESOURCE A | 2009 | Re search Policy2009，38，8： | 1 |
| 6 | Advanced im- aging in biology and medicine: technology, software environ- ments, applications 显示文摘 | Sensen Christoph W Benedikt H Khan Rao F H | 2009 | Med Phys2009，36，7： | 1 |
| 7 | Effects of using accurate climatic conditions for mechanistic-empirical pavement design显示文摘 | Tamer M B Christopher W R Daryl E H | 2011 | Journal of Transportation Engineering2011，137，： | 1 |
| 8 | Connell integrate the effects of climate change across entire systems that predicting ecosystem shifts requires new approaches显示文摘 | Bayden D R Christopher D G H Thomas W | | 0，，10： | 1 |
| 9 | Risk as feelings显示文摘 | boewenstein G F Elke U W Christopher K H Welch N | 2001 | Psychological Bulletin2001，127，2： | 1 |
| 10 | A novel introduction system for hydride generation-inductively coupled plasma mass spectrometry: determination of selenium in biological materials显示文摘 | Christoph Moor Joseph W H Lam Ralph E Sturgeon | | 0，，： | 1 |
| 11 | Proton radiography显示文摘 | CHRISTOPHER M JOHN W H PHLLIP G | 2006 | Los Alamos Science2006，，： | 1 |
| 12 | Liver cancer in low and middle income countries显示文摘 | Andraw J H Christopher P W | 2003 | BMJ2003，326，： | 1 |
| 13 | N-terminal pro-B-type natriuretic peptide,levels for dynamic risk stratification of patients with acute coronary syndromes显示文摘 | Christopher H Christian W Veselin M | 2004 | Circulation2004，110，： | 1 |
| 14 | Coarse woody debris in a montane forest in Ecuador: mass, C and nutrient stock, and turnover 显示文摘 | Wolfgang W Timo H Christoph B | 2005 | Forest Ecology and Management2005，205，1： | 1 |
| 15 | 显示文摘 | Christian S Kulicke W M Christoph H | 2004 | Starch/ Starke2004，56，5： | 1 |
| 16 | The draft genome of the transgenic tropical fruit tree papaya ( Carica pap aya Linnaeus ) 显示文摘 | MING R HOU S B FENG Y YU Q DIONNE-LAPORTE A SAW J H SENIN P WANG W LY B V LEWIS K L T SALZBERG S L FENG L GONES M R SKELTON R L MURRAYJ E CHEN C QIAN W SHEN J DU P EUSTICE M TONG E TANG H LYONS E PAULL R E MICHAEL T P WALL K RICE D W ALBERT H WANG M L ZHU Y J SCHATZ M NAGARAJAN N ACOB R A GUAN P BLAS A WAI C M ACKERMAN C M REN Y LIU C WANG J M WANG J P NA J SHAKIROV E V HAAS B THIMMAPURAM J NELSON D WANG X BOWERS J E GSCHWEND A R DELCHER A L SINGH R SUZUKI J Y TRIPATHI S NEUPANE K WEI H IRIKURA B PAIDI M JIANG N ZHANG W PRESTING G WINDSOR A NAVAJAS-PEREZ R TORRES M J FELTUS A PORTER B LI Y BURROUGHS A M LUO M C LIU L CHRISTOPHER D A MOUNT S M MOORE P H SUGIMURA T JIANG J SCHULER M A FRIEDMAN V MITCHELL-OLDS T SHIPPEN D E PAMPHILIS C W PALMER J D FREELING M PATERSON A H GONSALVES D WANG L ALAM M | 2008 | Nature2008，452，： | 1 |
| 17 | Patterns of Regional Inequality in the Enlarged Eu- rope显示文摘 | Martin H Christoph W | 2008 | European Sociological Review2008，24，1： | 1 |
| 18 | Interaction of the ACE D Allele and the GNB3 825T Allele in Myocardial Infarction显示文摘 | Christoph KN Johannes H Ulrich W | 2000 | Hypertension2000，36，： | 1 |
| 19 | Igneous Geology of the Carlin Trend, Nevada: Development of the Eocene Plutonic Complex and Significance for Carlin- Type Gold Deposits 显示文摘 | Ressel M W Christopher D H | 2006 | Economic Geology2006，，101： | 1 |
| 20 | Socio - economic patterns of sport demandand ggeing 显示文摘 | CHRISTOPH B KIRSTIN H PAMELA W etal | 2010 | Academic literature review2010，7，2： | 1 |
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