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您的检索式：作者名="Chuang Yaochen"
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| 1 | Effect of berberine on activity and mRNA expression of N-acetyl transferase in human lung cancer cell line A549显示文摘OBJECTIVE:Tostudy the effects of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell lineA549.METHODS: N-acetyltransferase antibodies were prepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate,and divided into the control group, and berberine0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups,with 3 wells for each group.24h later,A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse transcription polymerase chain reaction.RESULTS: The N-acetyltransferase content in human lung cancer A549 cells decreased with the increasing of berberine concentration, significantly lower than that in the control group(P<0.05 or P<0.001); and the mRNA expression of N-acetyltransferase also decreased with the increasing of berberine concentration, significantly lower in Huangliansu treatment groups(P<0.001).CONCLUSION: Berberine can inhibit the activity of N-acetyltransferase in human lung cancer cell line A549, and shows negative correlations of dose and time in a certain extent.The inhibited gene expression of N-acetyltransferase in human lung cancer A549 cell may probably represent one of the mechanisms for itsantineoplastic effect. | Shengyan Xi Kunhan Chuang Kwangming Fang Yimin Lee Jinggung Chung Yaochen Chuang | 2014 | Journal of Traditional Chinese Medicine2014，34，3： | 6 |
| 2 | A Method of layer-by-layer gold nanoparticle hybridization in a quartz crystal microbalance DNA sensing system used to detect dengue virus显示文摘 | Chen SH Chuang Yaochen Lu Yichen | 2009 | Nanotechnology2009，20，： | 1 |
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