|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Real-time and reliability analysis of time-triggered CAN-bus显示文摘Real-time performance and reliability are two most important issues in applications of time-triggered controller area network (CAN) bus systems at present. A scheduling matrix of time-triggered CAN-bus system is established using average-loading algorithm. Periodic messages are guaranteed to transmit without delay by distributing independent transmission windows within the system matrix. Considering the traditional CAN-bus transmission mechanism and the time-triggered feature, an algorithm is improved to calculate the worst-case delay of event-triggered messages in time-triggered CAN-bus systems. The failure probability is calculated for event-triggered messages whose worst-case delay exceeds their deadlines. Different levels of redundant structures of CAN-bus circuits are analyzed and the maintenance management is proposed to improve the system reliability. Finally, the reliabilities of different structures are calculated and the influences of maintenance on the system reliability are analyzed. | Xia Jiqiang Zhang Chuansen Bai Ronggang Xue Liqiang | 2013 | Chinese Journal of Aeronautics2013，26，1： | 17 |
| 2 | Lateral Flight Technical Error Estimation Model for Performance Based Navigation显示文摘Flight technical error (FTE) combined with navigation system error (NSE) is the main part of total system error (TSE) in performance based navigation (PBN).The implementation of PBN requires pre-flight prediction and en-route short-term dynamical prediction of the TSE.Once the sum of predicted lateral FTE and NSE is greater than the specified PBN value,the PBN cannot operate.Thus,accurate modeling and thorough analysis of lateral FTE are indispensible.Multiple-input multiple-output (MIMO) lateral track control system of a transport aircraft is designed using linear quadratic Gaussian and loop transfer recovery (LQG/LTR) method,and the lateral FTE of a turbulence disturbed approach operation is analyzed.The error estimation mapping function of latera FTE and its bound estimation algorithm are proposed based on singular value theory.According to the forming mechanism of lateral FTE,the algorithm considers environmental turbulence fluctuation disturbance,aircraft dynamics and control system parameters.Real-data-based Monte-Carlo simulation validates the theoretical analysis of FTE.It also shows that FTE is mainly caused by turbulence fluctuation disturbance when automatic flight control system (AFCS) is engaged and would increase with escalating environmental turbulence intensity. | ZHAO Hongsheng XU Xiaohao ZHANG Jun ZHU Yanbo YANG Chuansen HONG Sheng | 2011 | Chinese Journal of Aeronautics2011，24，3： | 8 |
| 3 | Using nerve segment insert grafting to reconstruct neural pathways of brain-derived paralysis显示文摘Since 1992,task groups have used free nerve auto grafts to bridge partially transected nerves from the dominant area of the normal cerebral cortex to nerves that innervate spastic muscles from a diseased cerebral cortex,using transplanting sutures to alleviate the muscle spasm of cerebral palsy caused by different diseases. This has facilitated rebuilding of some of its neurological function. In this study,80 such patients were followed up,including 20 patients with traumatic brain injury,32 patients with stroke,and 28 pediatric patients with cerebral palsy. After postoperative follow-up of 3 to 21 years,the efficacy rate of this operation was 100% and the excellent and good spasm relief rate was 82.5%. | Wenbin Ding Shaocheng Zhang Zhuo Wang Lin Chen Chuansen Zhang Ping Huang Shunfa Liu Laiqing Sun Yuhai Ma Jun Yang Fei Huang Chengjing Xue Yanxue Zhong Lei Yin Yongtai Pan Dajiang Wu | 2017 | Translational Neuroscience and Clinics2017，3，4： | 2 |
| 4 | Combined transplantation of bone marrow mesenchymal stem cells and pedicled greater omentum promotes locomotor function and regeneration of axons after spinal cord injury in rats显示文摘BACKGROUND: According to previous studies, the neuroprotective effect of the pedicled greater omentum may be attributed to the secretion of neurotrophic factors and stimulation of angiogenesis. The neurotrophic factors released from the pedicled greater omentum, such as brain-derived neurotrophic factor and neurotrophin 3/4/5 could exert a neuroprotective effect on the damaged host neural and glial cells, and also could induce the transdifferentiation of transplanted bone marrow mesenchymal stem cells (BMSCs) into neural cells. OBJECTIVE: Based on the functions of the omentum of neuro-protection and vascularization, we hypothesize that the transplantation of BMSCs and pedicled greater omentum into injured rat spinal cord might improve the survival rate and neural differentiation of transplanted BMSCs and consequently gain a better functional outcome. DESIGN, TIME AND SETTING: A randomized, controlled animal experiment. The experiments were carried out at the Department of Anatomy, the Secondary Military Medical University of Chinese PLA between June 2005 and June 2007. MATERIALS: Fifteen male inbred Wistar rats, weighing (200±20) g, provided by the Experimental Animal Center of the Secondary Military Medical University of Chinese PLA were used and met the animal ethical standards. Mouse anti-BrdU and mouse anti-NF200 monoclonal antibody were purchased from Boster, China. METHODS: Cell culture: We used inbred Sprague-Dawley rats to harvest bone marrow for culture of BMSCs and transplantation to avoid possible immune rejection. BMSCs were cultured via total bone marrow adherence. Experimental grouping and intervention: The rats were randomly divided into a control group, cell group and combined group, five rats per group. Rats in the control group underwent spinal cord injury (SCI) only, during which an artery clamp with pressure force of 30 g was employed to compress the spinal cord at the T10 level for 30 seconds to produce the SCI model. 5 μL PBS containing 105 BMSCs was injected into the injured site of the spinal cord in 60 seconds via a microsyringe in the cell group after SCI. In the combined group, after SCI and BMSC transplantation, an autograft pedicled greater omentum was transplanted onto the injured site of the spinal cord and fixed with a suture. SCI model and transplantation: Control group, SCI model without treatment; cell group, transplantation of BMSCs after SCI; combined group, combined transplantation of BMSCs and pedicled greater omentum after SCI. MAIN OUTCOME MEASURES: At days 1, 7, 14, 21 and 28 PO (post operation), the Basso, Beattie and Bresnahan (BBB) scale was used to observe and evaluate the recovery of locomotor function. At day 29 PO, after transcardial perfusion using 4% paraformaldehyde, a spinal cord segment of 1 cm around the injury was harvested. A cryostat section was performed longitudinally in the horizontal plane and sections were chosen by systematic random sampling for staining. Anti-BrdU staining and counting was performed to measure survival rate of transplanted BMSCs; anti-BrdU-nestin and BrdU-glial fibrillary acidic protein (GFAP) double staining and counting measured neural differentiation of BMSCs; and anti-NF 200 staining was used to evaluate axonal regeneration. RESULTS: All 15 rats were included in the outcome analysis, without any loss. Changes in BBB scores: Combined transplantation of BMSCs and the pedicled greater omentum produced significantly higher BBB scores at 7-28 days post-injury than in the control group (P < 0.05). BBB scores in the cell group were higher than in the control group at 28 days post-injury (P < 0.05). Survival rate and neural differentiation of transplanted BMSCs: Immunostaining of BrdU demonstrated that transplanted BMSCs survived in the spinal cord and migrated cranially and caudally as far as 0.5 mm from the injection site in the cell group and combined group. Some of the transplanted BMSCs expressed nestin or GFAP which revealed neural differentiation of BMSCs in the combined group and cell group. Axonal regeneration: The areas of axonal NF200 staining in the cell group and control group were lower than that of the combined group (P < 0.01). CONCLUSION: It is effective and feasible to transplant BMSCs with the pedicled greater omentum for regeneration of spinal cord after SCI compared with transplanting BMSCs alone. This method results in better locomotor outcomes and axonal regeneration. | Liang Li Zhiying Zhang Haiyan Lin Congli Ren Chuansen Zhang | 2008 | Neural Regeneration Research2008，3，4： | 2 |
| 5 | Comparative profiling of microRNA expression between neural stem cells and motor neurons in embryonic spinal cord in rat 显示文摘 | Hongen Wei Chunfang Wang Chuansen Zhang | 2010 | International Journal of Developmental Neuroscience2010，28，6： | 1 |
| 6 | Bridging sciatic nerve gap using tissue-engineered nerves constructed with neural tissue-committed stem cells derived from bone marrow显示文摘BACKGROUND:Schwann cells are the most commonly used cells for tissue-engineered nerves. However,autologous Schwann cells are of limited use in a clinical context,and allogeneic Schwann cells induce immunological rejections.Cells that do not induce immunological rejections and that are relatively easy to acquire are urgently needed for transplantation. OBJECTIVE:To bridge sciatic nerve defects using tissue engineered nerves constructed with neural tissue-committed stem cells(NTCSCs) derived from bone marrow;to observe morphology and function of rat nerves following bridging;to determine the effect of autologous nerve transplantation, which serves as the gold standard for evaluating efficacy of tissue-engineered nerves. DESIGN,TIME AND SETTING:This randomized,controlled,animal experiment was performed in the Anatomical Laboratory and Biomedical Institute of the Second Military Medical University of Chinese PLA between September 2004 and April 2006. MATERIALS:Five Sprague Dawley rats,aged 1 month and weighing 100-150 g,were used for cell culture.Sixty Sprague Dawley rats aged 3 months and weighing 220-250 g,were used to establish neurological defect models.Nestin,neuron-specific enolase(NSE),glial fibrillary acidic protein (GFAP),and S-100 antibodies were provided by Santa Cruz Biotechnology,Inc.,USA.Acellular nerve grafts were derived from dogs. METHODS:All rats,each with 1-cm gap created in the right sciatic nerve,were randomly assigned to three groups.Each group comprised 20 rats.Autograft nerve transplantation group:the severed 1-cm length nerve segment was reverted,but with the two ends exchanged;the proximal segment was sutured to the distal sciatic nerve stump and the distal segment to the proximal stump.Blank nerve scaffold transplantation group:a 1-cm length acellular nerve graft was used to bridge the sciatic nerve gap.NTCSC engineered nerve transplantation group:a 1-cm length acellular nerve graft,in which NTCSCs were inoculated,was used to bridge the sciatic nerve gap. MAIN OUTCOME MEASURES:Following surgery,sciatic nerve functional index and electrophysiology functions were evaluated for nerve conduction function,including conduction latency,conduction velocity,and action potential peak.Horseradish peroxidase(HRP,20%) was injected into the gastrocnemius muscle to retrogradely label the L_4 and L_5 nerve ganglions,as well as neurons in the anterior horn of the spinal cord,in the three groups.Positive expression of nestin, NSE,GFAP,and S-100 were determined using an immunofluorescence double-labeling method. RESULTS:NTCSCs differentiated into neuronal-like cells and glial-like cells within 12 weeks after NTCSC engineered nerve transplantation.HRP retrograde tracing displayed a large amount of HRP-labeled neurons in L_(4-5) nerve ganglions,as well as the anterior horn of the spinal cord,in both the autograft nerve transplantation and the NTCSC engineered nerve transplantation groups. However,few HRP-labeled neurons were detected in the blank nerve scaffold transplantation group. Nerve bridges in the autograft nerve transplantation and NTCSC engineered nerve transplantation groups exhibited similar morphology to normal nerves.Neither fractures or broken nerve bridges nor neuromas were found after bridging the sciatic nerve gap with NTCSCs-inoculated acellular nerve graft,indicating repair.Conduction latency,action potential,and conduction velocity in the NTCSC engineered nerve transplantation group were identical to the autograft nerve transplantation group (P>0.05),but significantly different from the blank nerve scaffold transplantation group(P<0.05). CONCLUSION:NTCSC tissue-engineered nerves were able to repair injured nerves and facilitated restoration of nerve conduction function,similar to autograft nerve transplantation. | Zhiying Zhang Congli Ren Chuansen Zhang Fang Liu Liang Li | 2009 | Neural Regeneration Research2009，4，5： | 1 |
| 7 | New algorithm for receiver autonomous integrity monitoring显示文摘 | YANG CHUANSEN XU XIAOHAO LIU RUIHUA | | 0，，02： | 1 |
| 8 | miR-124 and miR-128 differential expression in bone marrow stromal cells and spinal cord-derived neural stem cells显示文摘BACKGROUND: MicroRNA (miRNA) expression in stem cells provides important clues for the molecular mechanisms of stem cell proliferation and differentiation. Bone marrow stromal cells and spinal cord-derived neural stem cells exhibit potential for neural regeneration. However, miRNA expression in these cells has been rarely reported. OBJECTIVE: To explore differential expression of two nervous system-specific miRNAs, miR-124 and miR-128, in bone marrow stromal cells and spinal cord-derived neural stem cells. DESIGN, TIME AND SETTING: An In vitro, cell biology experiment was performed at the Department of Biotechnology, Shanxi Medical University from June 2008 to June 2009. MATERIALS: TaqMan miRNA assays were purchased from Applied Biosystems. METHODS: Rat bone marrow stromal cells were isolated and cultured using the whole-bone marrow method, and rat spinal cord-derived neural stem cells were obtained through neurosphere formation. TaqMan miRNA assays were used to measure miR-124 and miR-128 expression in bone marrow stromal cells and spinal cord-derived neural stem cells. MAIN OUTCOME MEASURES: Morphology of bone marrow stromal cells and spinal cord-derived neural stem cells were observed by inverted microscopy. Expression of the neural stem cell-specific marker, nestin, the bone marrow stromal cell surface marker, CD71, and expression of miR-124 and miR-128, were detected by real-time polymerase chain reaction. RESULTS: Cultured bone marrow stromal cells displayed a short fusiform shape. Flow cytometry revealed a large number of CD71-positive cells (> 95%). Cultured spinal cord-derived neural stem cells formed nestin-positive neurospheres, and quantitative detection of miRNA demonstrated that less miR-124 and miR-128 was expressed in bone marrow stromal cells compared to spinal cord-derived neural stem cells (P < 0.05). CONCLUSION: Bone marrow stromal cells and spinal cord-derived neural stem cells exhibited differential expression of miR-124 and miR-128, which suggested different characteristics in miRNA expression. | Chunfang Wang Hongen Wei Chuansen Zhang Pengfei Li Fei Wang Shufeng Han | 2010 | Neural Regeneration Research2010，5，11： | 1 |
| 9 | Isolation,culture and characterization of cardiac progenitor cells derived from human embryonic heart tubes显示文摘 | Zhang Xi Zhang Chuansen Liu Yanchun | | 0，，04： | 1 |
| 10 | Efficient induction of antit umor T cell immunity by exosomes derived from heat-shocked lymphoma cells显示文摘 | Weilin Chen Jianli Wang Chuansen Shao | 2006 | E ur J Immunol2006，36，： | 1 |
| 11 | Comparative profiling of microRNA expression between neural stem cells and motor neurons in embryonic spinal cord in rat显示文摘 | Hongen Wei Chunfang Wang Chuansen Zhang | | 0，，06： | 1 |
| 12 | Protective effects of hydrogen-rich saline on ulcerative colitis rat model显示文摘 | Jinghu He Shaohu Xiong Jianquan Zhang Junwu Wang Aijun Sun Xiang Mei Xuejun Sun Chuansen Zhang Qiang Wang | 2013 | Journal of Surgical Research2013，，： | 1 |
| 13 | Inhibitory effect of electro-acupuncture on apoptosis after spinal cord injury显示文摘BACKGROUND: Acupuncture and electro-acupuncture (EA) as complementary and alternative medicine have been applied for neurological recovery after spinal cord injury (SCI), but the mechanism of treatment is unclear yet. OBJECTIVE: To examine and compare the effects of EA with methylprednisolone (MP) on apoptosis and expression of caspase-3 mRNA and protein in early SCI, in an attempt to provide experimental clues for clinical use of EA in the treatment of early SCI. DESIGN: A randomized control trial. SETTING: Department of Anatomy, Second Military Medical University of Chinese PLA. MATERIALS: Forty-eight adult male Sprague-Dawley (SD) rats aged 12 weeks and weighing (250±20) g were used. The experimental procedures were performed in accordance with the animal care guidelines of the National Institute of Health (NIH). Equipments and medicine: Methylprednisolone sodium succinate (Pharmacia & Upjohn N.V./S.A., Puurs, Belgium); sodium pentobarbital (Sigma Chemical Co., St. Louis, MO); G-6805-2 Multi-Purpose Health Device (Shanghai Medical Instruments High-TECH Co., Shanghai, China); POD kit (Roche Molecular Biochemicals, Mannheim, Germany); ISH Detection Kit (Wuhan Boster Biological Technology Ltd., Wuhan, China); ABC kit (Vector Laboratories, Burlingame, CA); light microscope (×200, Olympus, Tokyo, Japan); HPIAS-1000 color High-definition pathological image analysis system (Qianping Imaging Engineering Company of Wuhan Tongji Medical University). METHODS: The experiment was carried out in Department of Anatomy, Second Military Medical University of Chinese PLA between October 2004 and March 2006. ① The SCI model was established by modified Allen’s method on T10. The force applied in traumatization was 50 g·cm. ② Forty-eight SD rats were equally randomized into four groups: sham operation (SO) group, in which animals received laminectomy only; model control (MC) group as a negative control, in which animals did not receive any treatment; EA treatment (EA) group, in which animals were treated with electro-acupuncture immediately after SCI, and MP treatment (MP) group, in which animals were treated with 30 mg/kg methylprednisolone sodium succinate through the tail vein immediately after SCI. Electro-acupuncture treatment was performed for EA group at 8 hours and 20 hours after SCI. The needles were inserted at a depth about 5-6 mm into the locus of the Dazhui and Mingmen. Asymmetric waves were used for electro-acupuncture at a stimulating frequency of 1 Hz. The total duration of EA stimulation was 30 minutes. ③ Six rats of each group were sacrificed at 6 hours and 24 hours after SCI. A 1.5 cm segment of the spinal cord encompassing the injury site was removed and prepared for experiment. ④ Cellular apoptosis was observed by terminal deoxynucleotidyl transferase (TDT)-mediated deoxyuridine triphosphate (dUTP)-biotin nick end labeling (TUNEL) method. Expression of caspase-3 mRNA and protein were estimated by in situ hybridization, immunohistochemistry and imaging analysis. ⑤ Measurement data among groups were compared with one-way analysis of variance and differences between groups were compared with q test. MAIN OUTCOME MEASURES: The effect of electro-acupuncture on TUNEL-positive cells, the expression of caspase-3 mRNA and protein. RESULTS: The forty-eight rats were involved in the final experiment. ① TUNEL-positive cells: TUNEL-positive cells in MP group and EA group were 29.00±8.27 and 30.17±6.85, respectively, at 6 hours after SCI; and were 1.00±0.89, 60.17±7.83, 6.67±3.56; 1.17±1.17, 69.50±8.50, 9.67±4.97, respectively at tip, intermediate zone and end of spinal cord at 24 hours after SCI; which were less than those in MC group (47.17±11.12, 9.33±4.76, 92.00±11.14, 22.50±5.43, P < 0.01). Cells in MP group were close to those in EA group (P > 0.05). ② Expression of positive caspase-3 mRNA: At 6 hours after SCI, numbers of positive caspase-3 mRNA were 133.33±11.84, 117.17±11.70, 120.00±10.73, respectively in MC, MP and EA groups, which were more than those in SO groups (83.50±8.64, P < 0.01). At 24 hours after SCI, numbers of positive caspase-3 mRNA were 121.50±11.34, 96.16±8.18, 97.67±9.69, respectively in MC, MP and EA groups, which were more than those in SO groups (83.50±9.64, P < 0.05-0.01). Numbers in MP group were close to those in EA group but less than those in MC group (P < 0.05-0.01). ③ Expression of caspase-3 protein: Numbers in MC and MP groups were close to those in EA group (12.83±2.86, 10.00±3.58, 10.50±3.62) at 6 hours after SCI; numbers in MP and EA groups were 11.00±4.43 and 12.17±3.43 at 24 hours after SCI, which were less than those in MC group (17.83±4.92, P < 0.01, 0.05). CONCLUSION: EA may partially inhibit apoptosis and protect nerve after SCI by down-regulating expression of caspase-3 mRNA and protein. | Zhiying Zhang Ruishan Dang Chuansen Zhang Liang Li Congli Ren Shuang Zhou Eryu Chen | 2006 | Neural Regeneration Research2006，1，3： | 0 |
| 14 | Role of neuregulin-1 in peripheral nerve injury显示文摘Peripheral nerve injury in humans often leads to permanent functional deficits.Schwann cells play an important role in the recovery of peripheral nerve injury by ensheathing axons and providing various neurotrophic factors.Neuregulin-1 (NRG-1) provides axonal signals, which allow dedifferentiation and rapid proliferation of Schwann cells.Subsequently, NRG-1 promotes axonal myelination and influences myelin thickness.Moreover, NRG-1 plays a critical role in synapse formation in the neuromuscular junction.These effects, together, suggest that NRG-1 promotes recovery of peripheral nerve injury. | Zhengdong Kong Zhiying Zhang Haiyan Lin Chuansen Zhang | 2010 | Neural Regeneration Research2010，5，14： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员