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| 1 | A Hu sheep genome with the first ovine Y chromosome reveal introgression history after sheep domestication显示文摘The Y chromosome plays key roles in male fertility and reflects the evolutionary history of paternal lineages.Here,we present a de novo genome assembly of the Hu sheep with the first draft assembly of ovine Y chromosome(o MSY),using nanopore sequencing and Hi-C technologies.The o MSY that we generated spans 10.6 Mb from which 775 Y-SNPs were identified by applying a large panel of whole genome sequences from worldwide sheep and wild Iranian mouflons.Three major paternal lineages(HY1a,HY1b and HY2)were defined across domestic sheep,of which HY2 was newly detected.Surprisingly,HY2 forms a monophyletic clade with the Iranian mouflons and is highly divergent from both HY1a and HY1b.Demographic analysis of Y chromosomes,mitochondrial and nuclear genomes confirmed that HY2 and the maternal counterpart of lineage C represented a distinct wild mouflon population in Iran that diverge from the direct ancestor of domestic sheep,the wild mouflons in Southeastern Anatolia.Our results suggest that wild Iranian mouflons had introgressed into domestic sheep and thereby introduced this Iranian mouflon specific lineage carrying HY2 to both East Asian and Africa sheep populations. | Ran Li Peng Yang Ming Li Wenwen Fang Xiangpeng Yue Hojjat Asadollahpour Nanaei Shangquan Gan Duo Du Yudong Cai Xuelei Dai Qimeng Yang Chunna Cao Weidong Deng Sangang He Wenrong Li Runlin Ma Mingjun Liu Yu Jiang | 2021 | Science China(Life Sciences)2021，64，7： | 1 |
| 2 | Mass spectrometry imaging and single-cell transcriptional profiling reveal the tissue-specific regulation of bioactive ingredient biosynthesis in Taxus leaves显示文摘Taxus leaves provide the raw industrial materials for taxol,a natural antineoplastic drug widely used in the treatment of various cancers.However,the precise distribution,biosynthesis,and transcriptional regulation of taxoids and other active components in Taxus leaves remain unknown.Matrix-assisted laser desorption/ionization–mass spectrometry imaging analysis was used to visualize various secondary metabolites in leaf sections of Taxus mairei,confirming the tissue-specific accumulation of different active metabolites.Single-cell sequencing was used to produce expression profiles of 8846 cells,with a median of 2352 genes per cell.Based on a series of cluster-specific markers,cells were grouped into 15 clusters,suggesting a high degree of cell heterogeneity in T.mairei leaves.Our data were used to create the first Taxus leaf metabolic single-cell atlas and to reveal spatial and temporal expression patterns of several secondary metabolic pathways.According to the cell-type annotation,most taxol biosynthesis genes are expressed mainly in leaf mesophyll cells;phenolic acid and flavonoid biosynthesis genes are highly expressed in leaf epidermal cells(including the stomatal complex and guard cells);and terpenoid and steroid biosynthesis genes are expressed specifically in leaf mesophyll cells.A number of novel and cell-specific transcription factors involved in secondary metabolite biosynthesis were identified,including MYB17,WRKY12,WRKY31,ERF13,GT_2,and bHLH46.Our research establishes the transcriptional landscape of major cell types in T.mairei leaves at a single-cell resolution and provides valuable resources for studying the basic principles of cell-type-specific regulation of secondary metabolism. | Xiaori Zhan Tian Qiu Hongshan Zhang Kailin Hou Xueshuang Liang Cheng Chen Zhijing Wang Qicong Wu Xiaojia Wang Xiao-lin Li Mingshuang Wang Shangguo Feng Houqing Zeng Chunna Yu Huizhong Wang Chenjia Shen | 2023 | Plant Communications2023，4，5： | 0 |
| 3 | Polymorphism analysis and supertype definition of swine leukocyte antigen class I molecules in three-way crossbred(Landrace,Duroc,and Yorkshire)pigs:implications for the vaccine development of African swine fever virus显示文摘Dear Editor,Swine major histocompatibility complex(MHC)is a highly polymorphic gene in pigs and is also called swine leukocyte antigen(SLA)(Fan et al.,2018).SLA is divided into three major categories,SLA I(SLA-1,-2,-3),SLA II,and SLA III(Smith et al.,2005).SLA I plays an important role in cellular immunity which can eliminate viruses and other foreign antigens in pigs(Macdonald et al.,2010).SLA-1,SLA-2 and SLA-3 are important SLA I alleles and are highly polymorphic.Polymorphism causes extreme difficulties in screening broadly protective cytotoxic lymphocyte(CTL)epitope antigens.The supertype which is built based on peptide-binding specificity of each SLA I can overcome this difficulty to some extent(Thomsen et al.,2013). | Limin Ba Zhenbao Wang William J Liu Dongxun Wu Wangzhen Xiang Peng Qi Chunna Dong Yanxin Hu Ping Lu Jin Xiao Changyuan Yu | 2020 | Science China(Life Sciences)2020，63，10： | 0 |
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