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| 1 | 过表达趋化因子受体2促进脂肪间充质干细胞对皮肤损伤的修复显示文摘目的探讨过表达趋化因子受体2(CCR2)是否能促进脂肪间充质干细胞(ADSCs)皮下移植对小鼠皮肤创伤修复的效率。方法分离培养ADSCs,取3~5代细胞通过流式细胞技术检测ADSCs相关的细胞表面标志物CD73、CD105、CD44和CD31的表达,并分别置于骨、软骨和脂肪诱导分化培养基中进行诱导分化,检测其多向分化潜能。通过慢病毒感染法构建过表达CCR2的ADSCs。在实验小鼠脊背两侧皮肤上分别制备一个面积为0. 8 cm×0. 8 cm的机械创面,随机分成3组,分别皮下注射CCR2-EGFP-ADSCs、EGFP-ADSCs和磷酸盐缓冲液(PBS)到小鼠创面4周。每天记录创面愈合情况,并进行病理切片观察,与对照组对比分析。结果分离培养的ADSCs高表达CD73、CD105和CD44,不表达CD31,具有骨、软骨、脂肪诱导分化潜能。过表达CCR2的ADSCs构建成功。小鼠皮肤创伤造模及移植细胞后,各组小鼠均存活,创面逐日缩小。CCR2-EGFP-ADSCs组、EGFP-ADSCs组和对照组第5、9天的创面愈合率分别为(68±3. 1)%和(91±2. 1)%、(63±2. 7)%和(87±2. 1)%、(58±3. 5)%和(80±2. 9)%。注射了ADSCs的两组愈合率均显著高于对照组(均P<0. 05)。病理组织切片显示3组小鼠第9天创面真皮层较正常皮层均有增厚,且CCR2-EGFP-ADSCs组观察到明显的类毛囊结构,多于EGFP-ADSCs组,对照组未观察到类似结构。结论过表达CCR2可促进ADSCs对皮肤损伤的修复。 | 祁旻龙 朱良 Yuri Ciervo 徐俊 | 2018 | 同济大学学报(医学版)2018，39，5： | 9 |
| 2 | Propagation through Optically Thick Media 显示文摘 | Ciervo A P | 1995 | Applied Optics1995，34，30： | 1 |
| 3 | Antibodies to 60-kilodalton heat shock protein and outer membrane protein 2 of Chlamydia pneumoniae in patients with coronary heart disease显示文摘 | Ciervo A Vista P Petrueea A | 2002 | Clin Diagn Lab Immunol2002，9，1： | 1 |
| 4 | T helper type 1 lymphocytes drive inflammation in human atherosclerotic lesions显示文摘 | BENAGIANO M AZZURRI A CIERVO A | 2003 | Proc Natl Acad Sci U S A2003，100，： | 1 |
| 5 | Antibody response to chlamydial heat shock protein 60 is strongly associated with acute coronary syndromes显示文摘 | Biasueei LM Liuzzo G Ciervo A | 2003 | Circulation2003，107，24： | 1 |
| 6 | Chlamydophila pneumoniae phospholipase D (CpPLD) drives Thl7 inflammation in human atherosclerosis 显示文摘 | Benagiano M Munari F Ciervo A | 2012 | Proc Natl Acad Sci U S A2012，109，4： | 1 |
| 7 | Antibodies to 60-kilodalton heat shock protein and outer membrane protein 2 of Chlamydia pneumonlae in patients with coronary heart disease显示文摘 | Ciervo A Visca P Petrucca A | 2002 | Clin and Diagn Lab Immu2002，9，1： | 1 |
| 8 | Chlamydophila pneumoniae phospholipase D (CpPLD) drives Thl7 inflammation in human atherosclerosis 显示文摘 | Benagiano M Munari F Ciervo A | 2012 | Proc Natl Aead Sei U S A2012，109，4： | 1 |
| 9 | 1037 HCV INFECTION IMPAIRS GLUCOSE METABOLISM WHICH PROMOTES A MORE AGGRESSIVE COURSE OF LIVER DISEASE AND METABOLIC SYNDROME显示文摘 | L.E. Adinolfi R. Zampino L. Restivo B. Guerrera A. Santoro A. Ciervo L. Rinaldi V.R. Scialdone M. Ferrara G. Ruggiero | 2010 | Journal of Hepatology2010，，： | 1 |
| 10 | Chlamydia pneumoniae in coronary atherosclerotic plaques and coronary instability显示文摘 | LIUZZO G CIERVO A NICCOLI G | 2011 | Int J Cardiol2011，147，： | 1 |
| 11 | Antibody response to chlamydial heat shock protein 60 is strongly associated with acute coronary syndromes显示文摘 | BIASUCCI L M LIUZZO G CIERVO A | 2003 | Circulation2003，107，24： | 1 |
| 12 | Evaluation and optimization of ELISA for detection of anti-Chlamydia pneumoniae IgG and IgA in patients with coronary heart diseases显示文摘 | Ciervo A Petrucca A Visca E | 2004 | J Microbiol Methods2004，59，1： | 1 |
| 13 | T helper type 1 lymphocytes drive inflammation in human atherosclerotic lesions 显示文摘 | Benagiano M Azzurri A Ciervo A | 2003 | Proc Natl Acad Sci USA2003，100，： | 1 |
| 14 | Chlamydophila pneumoniae, Mycoplasma pneumoniae, and influenza in children with respiratory infections in Alexandria, Egypt 显示文摘 | GRASSI T MANCINI F CIERVO A | 2014 | J Infect Dev Ctries2014，8，3： | 1 |
| 15 | Iron-regulated salicylate synthesis by Pseudomonas spp 显示文摘 | VISCA P CIERVO A SANFILIPPO V | 1993 | J Gen Microbiol1993，139，9： | 1 |
| 16 | The tourniquet revisited as an adjunct to lower limb revaseularization 显示文摘 | Ciervo A Dardik H Qin F | 2000 | J Vasc Surg2000，31，3： | 1 |
| 17 | Antibody response to chlamydial heat shock protein 60 is strongly associated with acute coronary syndromes 显示文摘 | Biosucci LM Liuzzo G Ciervo A | 2003 | Circulation2003，107，24： | 1 |
| 18 | Antibody response to chlamydial heat shock protein 60 is strongly associated with acute coronary syndromes 显示文摘 | Biasueei L M Liuzzo G Ciervo A | 2003 | Circulation2003，107，24： | 1 |
| 19 | Chlamy- dophila pneumoniae, Mycoplasma pneu- moniae, and influenza in children with re- spiratory infections in Alexandria, Egypt显示文摘 | Grassi T Mancini F Ciervo A | 2014 | J Infect Dev Ctries2014，8，3： | 1 |
| 20 | Chlamydophila pneumoniae, Mycoplasma pneumoniae, and influenza in children with respiratory infections in Alexandria, Egypt 显示文摘 | Grassi T Mancini F Ciervo A | 2014 | J Infect Dev Ctries2014，8，3： | 1 |
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