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| 1 | Assays for aptamer-based platforms显示文摘 | Marimuthu Citartan Subash C.B. Gopinath Junji Tominaga Soo-Choon Tan Thean-Hock Tang | 2012 | Biosensors and Bioelectronics2012，，1： | 1 |
| 2 | Assays for aptamer-based platforms 显示文摘 | Citartan M Gopinath S C Tominaga J | 2012 | Biosensors & Bioelec- tronics2012，34，1： | 1 |
| 3 | Assays for aptamer- based platforms显示文摘 | Citartan M Gopinath SC Tominag JJ | 2012 | Biosensors and Bioelectronics2012，34，1： | 1 |
| 4 | Assays for aptamer- based platforms显示文摘 | Citartan M Gopinath SC Tominaga J | 2012 | Biosensors and Bicelectronics2012，34，1： | 1 |
| 5 | Assays for aptamer-based platforms显示文摘 | Citartan M Gopinath SC Tominaga J | 2012 | Biosens Bioelectron2012，34，1： | 1 |
| 6 | Aptamers as a re- placement for antibodies in enzyme-linked immunosorbent assay 显示文摘 | Toh S Y Citartan M Gopinath S C B | 2015 | Biosens Bioelectron2015，64，： | 1 |
| 7 | Assays for aptamer-based platforms 显示文摘 | Citartan M Gopinath SC Tominaga J | 2012 | Biosens Bioelectron2012，34，1： | 1 |
| 8 | mRNA transcriptome profiling of human hepatocellular carcinoma cells HepG2 treated with Catharanthus roseus-silver nanoparticles显示文摘BACKGROUND The demand for the development of cancer nanomedicine has increased due to its great therapeutic value that can overcome the limitations of conventional cancer therapy.However,the presence of various bioactive compounds in crude plant extracts used for the synthesis of silver nanoparticles(AgNPs)makes its precise mechanisms of action unclear.AIM To assessed the mRNA transcriptome profiling of human HepG2 cells exposed to Catharanthus roseus G.Don(C.roseus)-AgNPs.METHODS The proliferative activity of hepatocellular carcinoma(HepG2)and normal human liver(THLE3)cells treated with C.roseusAgNPs were measured using MTT assay.The RNA samples were extracted and sequenced using BGIseq500 platform.This is followed by data filtering,mapping,gene expression analysis,differentially expression genes analysis,Gene Ontology analysis,and pathway analysis.RESULTS The mean IC 50 values of C.roseusAgNPs on HepG2 was 4.38±1.59μg/mL while on THLE3 cells was 800±1.55μg/mL.Transcriptome profiling revealed an alteration of 296 genes.C.roseusAgNPs induced the expression of stress-associated genes such as MT,HSP and HMOX-1.Cellular signalling pathways were potentially activated through MAPK,TNF and TGF pathways that are responsible for apoptosis and cell cycle arrest.The alteration of ARF6,EHD2,FGFR3,RhoA,EEA1,VPS28,VPS25,and TSG101 indicated the uptake of C.roseus-AgNPs via both clathrin-dependent and clathrinindependent endocytosis.CONCLUSION This study provides new insights into gene expression study of biosynthesised AgNPs on cancer cells.The cytotoxicity effect is mediated by the aberrant gene alteration,and more interestingly the unique selective antiproliferative properties indicate the C.roseusAgNPs as an ideal anticancer candidate. | Nur Asna Azhar Siti Aishah Abu Bakar Marimuthu Citartan Nor Hazwani Ahmad | 2023 | World Journal of Hepatology2023，15，3： | 0 |
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