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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | “Color Revolutions” and Beyond显示文摘“Color revolutions” (or “orange revolutions”) broke out in some of the Commonwealth of Independent States in the past couple of years, reminiscent of the drama of disintegration of the former Soviet bloc at the turn of the 1990s. Why those upheavals came hot on the heels of the Soviet collapses merely a dozen or so years ago? Are we expecting more domino effects down the road? What geographical repercussions will they spark? A panel discussion was held on the topic and their views published in the journal. | Dr. Ji Zhiye is Research Professor and Assistant President of China Institutes of Contemporary International Relations (CICIR), and Director of the Institute of Russian Studies concurrently. He is editor and coauthor of many books including Russian Think-Tanks on Foreign Affairs and The Shanghai Cooperation Organization——New Security and New Mechanism. Dr. Xu Tao is Research Professor and Deputy Director of the Institute of Russan Studies, CICIR. Jiang Li is Associate Research Professor of the Institute of Russian Studies, CICIR. Da Wei is Assistant Director of the Institute of American Studies, CICIR. Dr. Chen Xiangyang is Deputy Director of the Center for Strategic Studies, CICIR. Ding Xiaoxing is Assistant Research Professor of the Institute of Russian Studies, CICIR. | 2005 | Contemporary International Relations2005，15，6： | 3 |
| 3 | Security Situation in Afghanistan显示文摘In the first half of 2006, the Taliban armed forces intensified their terrorist attacks in Afghanistan, thus drawing attention of the international community. The worse situation there will inevitably produce negative impacts on the security in the west regions of China. Based on a thorough analysis of the situation, the author concluded that the low-intensity conflicts there would continue in some time to come. | Dr. Fang Jinying is an Associate Professor of the Institutes of Security and Strategic Studies, China Institutes of Contemporary International Relations (CICIR). Her major academic interests are in the worldwide Islamic extremism and South Asian security. She has written a number of articles. She is also the author of The Emergency and Development of “Ethnic Chinese Issue” in Southeast Asia (2001), and coauthor of Focus on Global Ethnic Issues (2001) and Focus on World Religious Issues (2003). | 2006 | Contemporary International Relations2006，16，6： | 2 |
| 4 | The NCEP/NCAR 40-year reanalysis project显示文摘 | KALNAY E COAUTHORS | 1996 | Bull Amer Meteor Soe1996，77，3： | 1 |
| 5 | GPSmeteorology:Direct estimation of the absolute value of precipitable water vapor 显示文摘 | Duan J Coauthors | 1996 | Meteor1996，35，： | 1 |
| 6 | Dropwindsonde observations for typhoon surveillance near the Taiwan region (DOTSTAR):An overview显示文摘 | Wu C C Coauthors | | 0，，： | 1 |
| 7 | The NCEP/NCAR 40-year reanalysis project显示文摘 | Kalnay E Coauthors | 1996 | Bull Amer Meteror Soc1996，77，3： | 1 |
| 8 | The NCEP-NCAR 50-year reanalysis: monthly means CD-ROM and documentation 显示文摘 | Kistler R Coauthors | 2001 | Bull Amer Meteror Soc2001，82，2： | 1 |
| 9 | The NCEP Climate Forecast System Reanalysis显示文摘 | Saha Suranjana Coauthors | 2010 | BullAmer Meteor Soc2010，91，： | 1 |
| 10 | Asymmetric trends in surface temperature 显示文摘 | KARL T R COAUTHORS | 1993 | Bull Amer Meteor Soc1993，74，： | 1 |
| 11 | Interannual variation of the rainfall in central Vietnam显示文摘 | YEN M C COAUTHORS | 2011 | Meteor Soc Japan2011，89，1： | 1 |
| 12 | Comparison of results from several AOGCMs for global and regional sea-level change 1900-2100显示文摘 | GREGORY J M Coauthors | 2001 | Climate Dyn2001，18，： | 1 |
| 13 | The RED experiment: an assement of boundary layer effect in a trade wind regime on microwave and infrared propagation over the sea 显示文摘 | Anderson K Coauthors | 2004 | Bull Amer Meteor Soc2004，85，9： | 1 |
| 14 | Investigating the causes of the response of the thermohaline circulation to past and future climate changes 显示文摘 | STOUFFER R Coauthors | 2006 | J Climate2006，19，： | 1 |
| 15 | The NCEP/NCAR 40-year reanalysis project 显示文摘 | Kalnay E Coauthors | 1996 | Bull Amer Meteor Soc1996，77，3： | 1 |
| 16 | The NCEP/NCAR 40-YearReanalysis Project显示文摘 | Kalnay E Coauthors | 1996 | Bull Amer Meteor Soc1996，77，： | 1 |
| 17 | Europe-wide reduction in primary pro- ductivity caused by the heat and drought in 2003显示文摘 | COAUTHORS C P | 2005 | Nature2005，437，： | 1 |
| 18 | Constraining global aiFsea gas exchange for COz with recent bomb 14C measurements显示文摘 | Sweeney C Coauthors | 2007 | Global Biogeochem Cycles2007，21，： | 1 |
| 19 | The NCEP/NCAR 40-year reanalysis project显示文摘 | KALNAY E COAUTHORS | 1996 | Bull Amer Meteor Soc1996，77，3： | 1 |
| 20 | Dynamical seasonal predic- tion显示文摘 | Shukla J Coauthors | 2000 | Bulletin of the American Meteorological Society2000，81，： | 1 |
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