1篇
您的检索式：作者名="Congyan Pan"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | ERp44 C160S/C212S mutants regulate IP_(3)R_(1) channel activity显示文摘Previous studies have indicated that ERp44 inhibits inositol 1,4,5-trisphosphate(IP3)-induced Ca2+release(IICR)via IP3R1,but the mechanism remains largely unexplored.Using extracellular ATP to induce intracellular calcium transient as an IICR model,Ca2+image,pull down assay,and Western blotting experiments were carried out in the present study.We found that extracellular ATP induced calcium transient via IP3Rs(IICR)and the IICR were markedly decreased in ERp44 overexpressed Hela cells.The inhibitory effect of C160S/C212S but not C29S/T396A/ΔT(331–377)mutants of ERp44 on IICR were significantly decreased compared with ERp44.However,the binding capacity of ERp44 to L3V domain of IP3R1(1L3V)was enhanced by ERp44 C160S/C212S mutation.Taken together,these results suggest that the mutants of ERp44,C160/C212,can more tightly bind to IP3R1 but exhibit a weak inhibition of IP3R1 channel activity in Hela cells. | Congyan Pan Ji Zheng Yanyun Wu Yingxiao Chen Likun Wang Zhansong Zhou Wenxuan Yin Guangju Ji | 2011 | Protein & Cell2011，2，12： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员