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您的检索式：作者名="Constance B.Bailey"
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| 1 | Corrigendum to“Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases”[Synth Syst Biotechnol 5(2)(2020)62–80]显示文摘The authors regret that following the publication of the original article,the authors noticed that some of the structures in Fig.10 were incorrect.Below is the corrected figure. | Erin E.Drufva Elijah G.Hix Constance B.Bailey | 2020 | Synthetic and Systems Biotechnology2020，5，4： | 4 |
| 2 | Engineered polyketides:Synergy between protein and host level engineering显示文摘Metabolic engineering efforts toward rewiring metabolism of cells to produce new compounds often require the utilization of non-native enzymatic machinery that is capable of producing a broad range of chemical functionalities.Polyketides encompass one of the largest classes of chemically diverse natural products.With thousands of known polyketides,modular polyketide synthases(PKSs)share a particularly attractive biosynthetic logic for generating chemical diversity.The engineering of modular PKSs could open access to the deliberate production of both existing and novel compounds.In this review,we discuss PKS engineering efforts applied at both the protein and cellular level for the generation of a diverse range of chemical structures,and we examine future applications of PKSs in the production of medicines,fuels and other industrially relevant chemicals. | Jesus F.Barajas Jacquelyn M.Blake-Hedges Constance B.Bailey Samuel Curran Jay.D.Keasling | 2017 | Synthetic and Systems Biotechnology2017，2，3： | 3 |
| 3 | Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases显示文摘Modular polyketide synthases(PKSs)are a multidomain megasynthase class of biosynthetic enzymes that have great promise for the development of new compounds,from new pharmaceuticals to high value commodity and specialty chemicals.Their colinear biosynthetic logic has been viewed as a promising platform for synthetic biology for decades.Due to this colinearity,domain swapping has long been used as a strategy to introduce molecular diversity.However,domain swapping often fails because it perturbs critical protein-protein interactions within the PKS.With our increased level of structural elucidation of PKSs,using judicious targeted mutations of individual residues is a more precise way to introduce molecular diversity with less potential for global disruption of the protein architecture.Here we review examples of targeted point mutagenesis to one or a few residues harbored within the PKS that alter domain specificity or selectivity,affect protein stability and interdomain communication,and promote more complex catalytic reactivity. | Erin E.Drufva Elijah G.Hix Constance B.Bailey | 2020 | Synthetic and Systems Biotechnology2020，5，2： | 1 |
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