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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Auction design in day-ahead electricity market (Republished)显示文摘 | Contreras J Candiles O Ignacio J | 2001 | IEEE Transactions on Power System2001，16，3： | 2 |
| 3 | Geneticmodification of liver grafts with an adenoviral vector encoding the Bcl-2 geneimproves organ preservation显示文摘 | Bilbao G Contreras JL Gomez Navarro J Eckhoff DE Mikheeva G Krasnykh V Hynes T Thomas FT Thomas JM Curiel DT | | 0，，： | 2 |
| 4 | Optical and crystallization behavior of TiO2 and V/TiO2 thin films prepared by plasma and ion beam assisted methods显示文摘 | Holgado J P Contreras L | 2003 | Thin Solid films2003，429，12： | 1 |
| 5 | 显示文摘 | Contreras J R Kohlstedt H | 2003 | Appl Phys Lett2003，83，： | 1 |
| 6 | Simulation and Evaluation of Optimization Problem Solutions in Distributed Energy Management Systems显示文摘 | Contreras J Losi A Russo M | 2002 | IEEE Trans on Power Systems2002，17，1： | 1 |
| 7 | Diode characteristics in state-of-the-art ZnO/CdS/Cu (In1-x Gax ) Se2 solar cells显示文摘 | Contreras M A Rarnanathan K Abushama J | 2005 | Progress in Photovoltaics : Research and Applications2005，13，： | 1 |
| 8 | Arsenic interactions during co-combustion processes based on thermodynamic equilibrium calculations显示文摘 | CONTRERAS M L AROSTEGUI J M ARMESTO L | 2009 | Fuel2009，88，3： | 1 |
| 9 | Phenylmercury degradation by heterogeneous photocatalysis assisted by UV-A light显示文摘 | Miranda C Yanez J Contreras D | 2013 | Journal of Environmental Science and Health Part A2013，48，13： | 1 |
| 10 | Pharmacokinetics of inhaled rifampicin porous particles for tuberculosis treatment: insight into rifampicin absorption from the lungs of guinea pigs显示文摘 | Garcia Contreras L Sung J Ibrahim M | 2015 | Mol Pharrn2015，12，8： | 1 |
| 11 | Morphofuric- tional disturbance of human sperm after incubation with organophospho- rate pesticides 显示文摘 | CONTRERAS HR BADILLA J BUSTOS-OBREG N E | 1999 | Biocell1999，23，2： | 1 |
| 12 | Mierostructural evolution during large strain hot rolling of an AM60 alloy 显示文摘 | PEREZ - PRADO M T VALLE J A CONTRERAS J M RUANOO A | 2004 | Scripta Mater2004，50，5： | 1 |
| 13 | A GARCH forecasting model to predict day-ahead electricity prices显示文摘 | CARCIA R C CONTRERAS J AKKEREN M | 2005 | IEEE Trans on Power Systems2005，20，2： | 1 |
| 14 | Diode characteristics in state-of-the-art ZnO/CdS/Cu (In1-xGax)Se2 solar cells 显示文摘 | CONTRERAS M RAMANATHAN K ABUSHAMA J HASOON F YOUNG D EGAAS B NOUFI R | 2005 | Prog Photovolt: Res Appl2005，13，： | 1 |
| 15 | Forecasting next-day electricity prices by time series models显示文摘 | Nogales F J Contreras J Conejo A J | 2002 | IEEE Transaction on Power Systems2002，17，2： | 1 |
| 16 | Kullback Leibler divergence measure for multivariate skewnormal distributions显示文摘 | Contreras Reyes J E Arellano Valle R B | 2012 | Entropy2012，14，： | 1 |
| 17 | Incidence of Oral Mucositis, Its Treatment and Pain Management in Patients Receiving Cancer Treatment at Radiation Oncology Depart- ments in Spanish Hospitals (MUCODOL Study)显示文摘 | Manas A Palacios A Contreras J | 2009 | Clin Transl Oncol2009，11，10： | 1 |
| 18 | Microstmcture and properties of hercynite- magnesia-calcium zirconate refractory mixtures 显示文摘 | Contreras J E Castillo G A | 2005 | Materials Characterization2005，54，11： | 1 |
| 19 | Herpesviruses in periodontal pocket and gingival tissue specimens显示文摘 | Nowzari H Slots J | 2000 | Oral Microbiol Immunol JT-Oral microbiology and immunology2000，15，1： | 1 |
| 20 | In vivo transgenic bioassays and assessment of the carcinogenic potential of pharmaceuticals 显示文摘 | CONTRERA J F DEGEORGE J J | 1998 | Environ Health Perspect1998，106，1： | 1 |
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