4篇
您的检索式：作者名="Cui Nanfang"
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| 1 | Contribution of elF-4E inhibition to the expression and activity of heparanase in human colon adenocarcinoma cell line:LS-174T显示文摘AIM: Heparanase degrades heparan sulfate proteoglycans (HSPGs) and is a critical mediator of tumor metastasis and angiogenesis. Recently, it has been cloned as a single gene family and found to be a potential target for antimetastasis drugs. However, the molecular basis for the regulation of heparanase expression is still not quite clear. The aim of this study was to determine whether the expression of eukaryotic initiation factor 4E (eIF-4E) correlated with the heparanase level in tumor cells and to explore the correlation between heparanase expression and metastatic potential of LS- 174T cells.METHODS: A 20-met antisense s-oligodeoxynucleotide (asODN) targeted against the translation start site of eIF-4E mRNA was introduced into LS-174T cells by lipid-mediated DNA-transfection. eIF-4E protein and mRNA levels were detected by Western blot analysis and RT-PCR, respectively.Heparanase activity was defined as the ability to degrade high molecular weight (40-100 kDa) radiolabeled HS (heparan sulfate) substrate into low molecular weight (5-15 kDa) HS fragments that could be differentiated by gel filtration chromatography. The invasive potential of tumor cell in vitro was observed by using a Matrigel invasion assay system.RESULTS: The 20-mer asODN against eIF-4E specifically and significantly inhibited eIF-4E expression at both transcriptional and translational levels. As a result, the expression and activity of heparanase were effectively retarded and the decreased activity of heparanase resulted in the decreased invasive potential of LS-174T.CONCLUSION: eIF-4E is involved in the regulation of heparanase production in colon adenocarcinoma cell line LS-174T, and its critical function makes it a particularly interesting target for heparanase regulation. This targeting strategy in antisense chemistry may have practical applications in experimental or clinical anti-metastatic gene therapy of human colorectal carcinoma. | Yu-Jie Yang Ya-Li Zhang Xu Li Han-Lei Dan Zhuo-Sheng Lai Ji-De Wang Qun-Ying Wang Hai-Hong Cui Yong Sun Ya-Dong Wang, Chinese PLA Institute of Digestive Disease, Nanfang Hospital, First Military Medical University, Guangzhou 510515, Guangdong Province, China | 2003 | World Journal of Gastroenterology2003，9，8： | 10 |
| 2 | Buffer sizing approach with dependence assumption between activities in critical chain scheduling显示文摘 | Li Bie Nanfang Cui Xiaoming Zhang | 2012 | International Journal of Production Research2012，，24： | 1 |
| 3 | Buffer sizing approach with dependence assumption between activi- ties in critical chain scheduling显示文摘 | Bie Li Cui Nanfang Zhang Xiaoming | 2012 | International Jour- nal of Production Research2012，50，24： | 1 |
| 4 | Effective expediting to improve project due date and cost performance through buffer management显示文摘 | Xuejun Hu Nanfang Cui Erik Demeulemeester | 2015 | International Journal of Production Research2015，53，5： | 1 |
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